Construct: sgRNA BRDN0001147030
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTTCGATGACACCCCTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK1 (57143)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76085
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
77887127 |
+ |
ADCK1 |
NNNTCGATGACACCCCTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
91953410 |
+ |
GPC5 |
NNNTCTATGTCACCCCTCTG |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000002.12 |
2 |
218280667 |
+ |
PNKD |
NNNTGGCTGACACCCCTCTG |
NGG |
2 |
0.2625 |
Tier II |
| 4 |
NC_000002.12 |
2 |
218280667 |
+ |
TMBIM1 |
NNNTGGCTGACACCCCTCTG |
NGG |
2 |
0.2625 |
Tier II |
| 5 |
NC_000011.10 |
11 |
1291717 |
+ |
TOLLIP |
NNNTCGCTGACCCCCCTCTG |
NGG |
2 |
0.1151 |
Tier II |
| 6 |
NC_000009.12 |
9 |
130699031 |
- |
EXOSC2 |
NNNTCCATGACAGCCCTCTG |
NGG |
2 |
0.093 |
Tier II |
| 7 |
NC_000013.11 |
13 |
45843342 |
- |
SIAH3 |
NNNTCGATGACACCACTCTG |
NGA |
2 |
0.0154 |
Tier II |
| 8 |
NC_000011.10 |
11 |
112525977 |
- |
LINC02763 |
NNNTCTATGACTCCCCTCTG |
NGG |
2 |
0.2222 |
Tier III |
| 9 |
NC_000008.11 |
8 |
127899585 |
+ |
PVT1 |
NNNTGGATGACACCACTCTG |
NGG |
2 |
0.1333 |
Tier III |
| 10 |
NC_000017.11 |
17 |
63913858 |
- |
LOC112268204 |
NNNTCGATGACACCCCTCTT |
NGA |
2 |
0.0486 |
Tier III |
| 11 |
NC_000012.12 |
12 |
93273801 |
- |
LOC643339 |
NNNTTGATGACACCTCTCTG |
NGG |
2 |
0.0424 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
88431057 |
+ |
Adck1 |
NNNTCGATGACACCCCTCTT |
NGG |
1 |
0.7 |
Tier I |
| 2 |
NC_000070.6 |
4 |
117262581 |
- |
Tmem53 |
NNNTAGGTGACACCCCTCTG |
NGG |
2 |
0.4034 |
Tier II |
| 3 |
NC_000083.6 |
17 |
30484341 |
- |
Btbd9 |
NNNTCAATGACAACCCTCTG |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000071.6 |
5 |
117721523 |
+ |
Ksr2 |
NNNTCCTTGACACCCCTCTG |
NGG |
2 |
0.2983 |
Tier II |
| 5 |
NC_000078.6 |
12 |
82969621 |
- |
Rgs6 |
NNNTTGATGCCACCCCTCTG |
NGG |
2 |
0.2121 |
Tier II |
| 6 |
NC_000072.6 |
6 |
122841505 |
- |
Foxj2 |
NNNTGGATGACACCCCTCTG |
NTG |
2 |
0.0234 |
Tier II |
| 7 |
NC_000086.7 |
X |
99289676 |
- |
Gm30649 |
NNNTCCATGACACCCCTCTA |
NGG |
2 |
0.6392 |
Tier III |
| 8 |
NC_000086.7 |
X |
99289676 |
- |
Gm39528 |
NNNTCCATGACACCCCTCTA |
NGG |
2 |
0.6392 |
Tier III |
| 9 |
NC_000085.6 |
19 |
36638219 |
- |
Hectd2os |
NNNTCTATGTCACCCCTCTG |
NGG |
2 |
0.5882 |
Tier III |
Other clones with same target sequence:
(none)
