Construct: sgRNA BRDN0001147032
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTACATCGTGATGAACTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK11A (728642)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75891
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
1704607 |
- |
CDK11A |
NNNCATCGTGATGAACTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
1637465 |
- |
CDK11B |
NNNCATCGTGATGAACTATG |
NGG |
1 |
0.4615 |
Tier I |
| 3 |
NC_000015.10 |
15 |
43989721 |
- |
FRMD5 |
NNNCATCGTGATGGACTACG |
NTG |
2 |
0.0182 |
Tier II |
| 4 |
NC_000015.10 |
15 |
43989721 |
- |
ACTBP7 |
NNNCATCGTGATGGACTACG |
NTG |
2 |
0.0182 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
155647911 |
+ |
Cdk11b |
NNNCATTGTGATGAACTACG |
NGG |
1 |
0.8125 |
Tier I |
| 2 |
NC_000070.6 |
4 |
138863423 |
- |
Pla2g5 |
NNNCACCGTGATGAACTACA |
NGG |
2 |
0.8523 |
Tier II |
| 3 |
NC_000071.6 |
5 |
102429366 |
+ |
Arhgap24 |
NNNCATTGTGATGAACTACA |
NGG |
2 |
0.7617 |
Tier II |
| 4 |
NC_000077.6 |
11 |
52924452 |
- |
Fstl4 |
NNNCATCGTGATAAACTACT |
NGG |
2 |
0.6462 |
Tier II |
| 5 |
NC_000070.6 |
4 |
138863423 |
- |
Gm32930 |
NNNCACCGTGATGAACTACA |
NGG |
2 |
0.8523 |
Tier III |
Other clones with same target sequence:
(none)
