Construct: sgRNA BRDN0001147034

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGCCAAGATGTTGTGAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYLK (4638)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77022

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 123733810 - MYLK NNNCCAAGATGTTGTGAGCA NGG 0 1.0 Tier I
2 NC_000012.12 12 66853092 + GRIP1 NNNCCAAGATGTTATGAGCT NGG 2 0.45 Tier II
3 NC_000001.11 1 28273633 + SESN2 NNNCCAAGAGGTTCTGAGCA NGG 2 0.2143 Tier II
4 NC_000002.12 2 2090181 - MYT1L NNNCAAAGATGTTGTGAGCA NAG 2 0.1481 Tier II
5 NC_000011.10 11 77084333 + CAPN5 NNNCCAAGATGCTGTGAGGA NGG 2 0.1184 Tier II
6 NC_000023.11 X 72383486 + HDAC8 NNNCCAAGATGTTGTAAGCA NGA 2 0.0694 Tier II
7 NC_000003.12 3 188365644 - LPP NNNCCAAGATGTTTTGAGCA NCG 2 0.0286 Tier II
8 NC_000011.10 11 21362537 + NELL1 NNNCCAAGATGTTGTGGGCA NTG 2 0.0069 Tier II
9 NC_000007.14 7 36193179 - EEPD1 NNNCCAAGATGTGGTGAGCA NGC 2 0.0058 Tier II
10 NC_000015.10 15 40980571 - INO80 NNNCCAAGAAGTTGTTAGCA NGG 2 0.0 Tier II
11 NC_000023.11 X 113337189 + LOC101928437 NNNTCAAGATGATGTGAGCA NGG 2 0.64 Tier III
12 NC_000020.11 20 57116834 - LOC105372685 NNNCCAAGCTGCTGTGAGCA NGG 2 0.5414 Tier III
13 NC_000004.12 4 53711543 - LOC100506444 NNNCCAAGAAGTTGAGAGCA NGG 2 0.4962 Tier III
14 NC_000008.11 8 136672607 + LINC02055 NNNTAAAGATGTTGTGAGCA NGG 2 0.4571 Tier III
15 NC_000006.12 6 23619139 + LOC105374976 NNNCCAAGTTGTTGTGAACA NGG 2 0.4154 Tier III
16 NC_000002.12 2 133947073 - LOC105373627 NNNCCAAGATGTTTTGAGCA NGG 1 0.2667 Tier III
17 NC_000006.12 6 79854769 + LOC100422671 NNNCCAAGATATTGTGAGCA NAG 2 0.2593 Tier III
18 NC_000015.10 15 66986470 + SMAD3-DT NNNGCAAGATGTTGTGACCA NGG 2 0.2381 Tier III
19 NC_000024.10 Y 17813836 - OFD1P1Y NNNCTAAGATGTGGTGAGCA NGG 2 0.166 Tier III
20 NC_000024.10 Y 18092175 + OFD1P2Y NNNCTAAGATGTGGTGAGCA NGG 2 0.166 Tier III
21 NC_000010.11 10 24756628 + LOC105376456 NNNCCAAGATGTGCTGAGCA NGG 2 0.1118 Tier III
22 NC_000006.12 6 2251452 + GMDS-DT NNNCCAAGATGTTGTGTGTA NGG 2 0.0615 Tier III
23 NC_000015.10 15 40980571 - LOC124903476 NNNCCAAGAAGTTGTTAGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 149071033 - Pex14 NNNTCAGGATGTTGTGAGCA NGG 2 0.5647 Tier II
2 NC_000070.6 4 63398770 - Akna NNNCCTAGATGTCGTGAGCA NGG 2 0.5639 Tier II
3 NC_000080.6 14 22445168 + Lrmda NNNTCAAGATGTTGTGAACA NGG 2 0.5538 Tier II
4 NC_000070.6 4 128545162 + Csmd2 NNNCCAGGATGGTGTGAGCA NGG 2 0.3529 Tier II
5 NC_000085.6 19 22855668 - Trpm3 NNNCCAAGATGTTTTGATCA NGG 2 0.1778 Tier II
6 NC_000074.6 8 23982060 - Zmat4 NNNCAAAGATGTTGTGAGCA NAG 2 0.1481 Tier II
7 NC_000085.6 19 10405197 - Syt7 NNNCCAAGATGTGGTGAGAA NGG 2 0.1118 Tier II
8 NC_000078.6 12 99516370 + Foxn3 NNNCCAAGTTGTTGTGTGCA NGG 2 0.08 Tier II
9 NC_000071.6 5 72167102 + Commd8 NNNCCAAGATATTGTGAGCA NGA 2 0.0694 Tier II
10 NC_000071.6 5 110302301 - Pole NNNCCAACATGTTGTGAGCA NGA 2 0.0427 Tier II
11 NC_000084.6 18 73949311 - Mapk4 NNNCCAAGATGTTGTGAGCT NTG 2 0.0234 Tier II
12 NC_000071.6 5 130173838 - Rabgef1 NNNCCAAGATGTTGTAAGCA NGT 2 0.0161 Tier II
13 NC_000068.7 2 27396283 + Vav2 NNNCCAAGATATTGTCAGCA NGG 2 0.0 Tier II
14 NC_000069.6 3 154888849 + Tnni3k NNNCCAAGATCTTGTCAGCA NGG 2 0.0 Tier II
15 NC_000079.6 13 24894331 + D130043K22Rik NNNCCAAGATGTTGTCAACA NGG 2 0.0 Tier II
16 NC_000076.6 10 54067672 + Man1a NNNCCAAGATGTTTTCAGCA NGG 2 0.0 Tier II
17 NC_000070.6 4 63398770 - Gm52742 NNNCCTAGATGTCGTGAGCA NGG 2 0.5639 Tier III
18 NC_000077.6 11 62939188 + Gm36614 NNNCCAAGAGCTTGTGAGCA NGG 2 0.2143 Tier III
19 NC_000079.6 13 113191599 - Gm41069 NNNCCAAGGTGTTTTGAGCA NGG 2 0.1778 Tier III
20 NC_000084.6 18 45860663 - A330093E20Rik NNNCCAAGATGTTTTGAGCT NGG 2 0.16 Tier III
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Other clones with same target sequence:

(none)