Construct: sgRNA BRDN0001147036

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAAAGGCCAGACAATTCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K6 (5608)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76041

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 69520355 + MAP2K6 NNNAGGCCAGACAATTCCAG NGG 0 1.0 Tier I
2 NC_000007.14 7 71308559 - GALNT17 NNNGGGACAGACAATTCCAG NGG 2 0.4688 Tier II
3 NC_000012.12 12 111598598 + ATXN2 NNNAGGCCTGACAATCCCAG NGG 2 0.4 Tier II
4 NC_000002.12 2 231099686 - PSMD1 NNNAGGCAAGACATTTCCAG NGG 2 0.3467 Tier II
5 NC_000002.12 2 171039591 - TLK1 NNNAGGCCAGCAAATTCCAG NGG 2 0.2857 Tier II
6 NC_000005.10 5 32715146 - NPR3 NNNAGGCCAGCCAAATCCAG NGG 2 0.2316 Tier II
7 NC_000017.11 17 75631415 + RECQL5 NNNATGCCAGGCAATTCCAG NGG 2 0.195 Tier II
8 NC_000002.12 2 215085996 + ABCA12 NNNAGGCCAGACAATGACAG NGG 2 0.1615 Tier II
9 NC_000019.10 19 38352919 + CATSPERG NNNAGGCCAGACTATTCAAG NGG 2 0.1615 Tier II
10 NC_000023.11 X 110678158 - CHRDL1 NNNAGGCCAGAGAATGCCAG NGG 2 0.1538 Tier II
11 NC_000015.10 15 66310264 + DIS3L NNNATGCCAGAGAATTCCAG NGG 2 0.1333 Tier II
12 NC_000002.12 2 72203936 - EXOC6B NNNAGGCCAGAGAATTCCAG NAG 2 0.1152 Tier II
13 NC_000015.10 15 68629509 + CORO2B NNNAGGCCAGACTATGCCAG NGG 2 0.1038 Tier II
14 NC_000022.11 22 44748411 - PRR5-ARHGAP8 NNNAGACCAGACAATTGCAG NGG 2 0.0588 Tier II
15 NC_000012.12 12 117913343 - KSR2 NNNAGGACAGACAAGTCCAG NGG 2 0.0375 Tier II
16 NC_000006.12 6 143552814 + PHACTR2 NNNAGGCCAGAGAATTGCAG NGG 2 0.0261 Tier II
17 NC_000007.14 7 69756563 + AUTS2 NNNAGGCCAGACAACTCCAG NTG 2 0.0106 Tier II
18 NC_000018.10 18 76937108 - ZNF236 NNNAGGCCAGACAAGTCCAG NTG 2 0.0019 Tier II
19 NC_000002.12 2 38197885 + LOC102723739 NNNAGGCCAGACAATTCCTG NGG 1 0.5385 Tier III
20 NC_000011.10 11 3627466 - TRPC2 NNNAGGCCAGAAAATTCCTG NGG 2 0.3846 Tier III
21 NC_000014.9 14 19101730 - DUXAP9 NNNAGGCCAGATAATTCCAT NGG 2 0.3769 Tier III
22 NC_000014.9 14 19298298 + DUXAP10 NNNAGGCCAGATAATTCCAT NGG 2 0.3769 Tier III
23 NC_000014.9 14 19298298 + LINC01297-DUXAP10-NBEAP6 NNNAGGCCAGATAATTCCAT NGG 2 0.3769 Tier III
24 NC_000022.11 22 15824197 - DUXAP8 NNNAGGCCAGATAATTCCAT NGG 2 0.3769 Tier III
25 NC_000014.9 14 103121452 - LINC00677 NNNAGGCCAGGCACTTCCAG NGG 2 0.1393 Tier III
26 NC_000002.12 2 127813259 + LOC124907885 NNNAGGCCAGACAATTCCAG NTC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 110492981 + Map2k6 NNNAGGCCAAACAATTCCAG NGG 1 0.9333 Tier I
2 NC_000081.6 15 7834505 + Gdnf NNNAGCCCAGAGAATTCCAG NGG 2 0.303 Tier I
3 NC_000081.6 15 34897183 - Stk3 NNNAGGCCAAGCAATTCCAG NGG 2 0.6067 Tier II
4 NC_000068.7 2 110890044 + Ano3 NNNAGTCCAGACAATACCAG NGG 2 0.6061 Tier II
5 NC_000085.6 19 47136900 + Calhm2 NNNAGGCCAGACAAATCCAG NGG 1 0.5789 Tier II
6 NC_000078.6 12 37918645 + Dgkb NNNAAGCCAGACAAATCCAG NGG 2 0.5018 Tier II
7 NC_000070.6 4 126781548 + AU040320 NNNAGGCCTTACAATTCCAG NGG 2 0.4875 Tier II
8 NC_000085.6 19 30234786 - Mbl2 NNNAGGCAAGACAATCCCAG NGG 2 0.4333 Tier II
9 NC_000080.6 14 75250175 - Cpb2 NNNAGACCACACAATTCCAG NGG 2 0.4 Tier II
10 NC_000071.6 5 76152448 - Srd5a3 NNNAGGCCAGACAAACCCAG NGG 2 0.386 Tier II
11 NC_000072.6 6 91791931 + Grip2 NNNAGGCCAGACATTTCAAG NGG 2 0.2872 Tier II
12 NC_000073.6 7 118644280 - Tmc5 NNNTGGCCAGACAATTCCAG NAG 2 0.165 Tier II
13 NC_000070.6 4 98509248 - Patj NNNAGGCCAGACAGCTCCAG NGG 2 0.1273 Tier II
14 NC_000075.6 9 45552091 + Dscaml1 NNNAGGCCAGACACTTCCTG NGG 2 0.1154 Tier II
15 NC_000086.7 X 99825784 - Tmem28 NNNAGGCCAGATACTTCCAG NGG 2 0.1154 Tier II
16 NC_000075.6 9 123006051 + Kif15 NNNAGGCCAGACAAATCCAG NCG 2 0.062 Tier II
17 NC_000077.6 11 118316146 - Timp2 NNNAGGCCAGATAATTCCAG NGA 2 0.0374 Tier II
18 NC_000075.6 9 67226873 + Tln2 NNNAGGCCAGACCATTCCAG NTG 2 0.0082 Tier II
19 NC_000068.7 2 58308551 - Acvr1c NNNAGGGCAGACAATTCCAG NGT 2 0.0076 Tier II
20 NC_000069.6 3 41071030 + Pgrmc2 NNNAGGCCAGACAATTCCAG NAA 2 0.0 Tier II
21 NC_000067.6 1 59366121 - Gm51625 NNNAGGCAAGACAATTCCAA NGG 2 0.6094 Tier III
22 NC_000073.6 7 115026570 - Gm34225 NNNAGGCCAGAAAATTCAAG NGG 2 0.3846 Tier III
23 NC_000069.6 3 107713989 - Gm40121 NNNAGGCCAGACAGTTACAG NGG 2 0.2178 Tier III
24 NC_000078.6 12 13102440 + Gm35350 NNNAGGCCAGACCATTCCAA NGG 2 0.1974 Tier III
25 NC_000067.6 1 183804780 + Gm34068 NNNGGGCCAGACAACTCCAG NGG 2 0.1705 Tier III
26 NC_000077.6 11 108894226 - Gm46298 NNNAGGCCTGACAATTCCAG NGA 2 0.0417 Tier III
27 NC_000075.6 9 80708275 + Gm39380 NNNAGGCCAGACAATTTCAG NTG 2 0.0182 Tier III
28 NC_000068.7 2 45402416 + Gm13479 NNNAGGCCAGACAATTCCAG NCC 2 0.0 Tier III
29 NC_000075.6 9 90157889 + 9430062P05Rik NNNAGGCCAGACAATTCCAG NAC 2 0.0 Tier III
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Other clones with same target sequence:

(none)