Construct: sgRNA BRDN0001147045
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGTAACAGAAACGTGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK2 (5062)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77567
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
196814531 |
+ |
PAK2 |
NNNGGTAACAGAAACGTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
95245071 |
- |
UNC5C |
NNNGGTATCAGAAACGTACA |
NGG |
2 |
0.2967 |
Tier I |
| 3 |
NC_000011.10 |
11 |
77340684 |
- |
PAK1 |
NNNGGTGACAGAAACTTGCA |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000018.10 |
18 |
8808912 |
+ |
MTCL1 |
NNNGGGAACAGGAACGTGCA |
NGG |
2 |
0.4127 |
Tier II |
| 5 |
NC_000008.11 |
8 |
143041440 |
- |
LY6S |
NNNGGTAACAGCAACGTTCA |
NGG |
2 |
0.1754 |
Tier II |
| 6 |
NC_000006.12 |
6 |
57275861 |
+ |
PRIM2 |
NNNGGTAACAGAAACGTCCA |
NAG |
2 |
0.1235 |
Tier II |
| 7 |
NC_000018.10 |
18 |
11863768 |
+ |
GNAL |
NNNGGAAACAGAAATGTGCA |
NGG |
2 |
0.0578 |
Tier II |
| 8 |
NC_000005.10 |
5 |
72115058 |
+ |
MAP1B |
NNNGGTAACAGAAAAGTGCC |
NGG |
2 |
0.0505 |
Tier II |
| 9 |
NC_000003.12 |
3 |
50933412 |
- |
DOCK3 |
NNNGGTAACAGAAAGGTGCT |
NGG |
2 |
0.03 |
Tier II |
| 10 |
NC_000003.12 |
3 |
97284037 |
- |
EPHA6 |
NNNGGTAACAGAAATGTGCA |
NAG |
2 |
0.0173 |
Tier II |
| 11 |
NC_000007.14 |
7 |
2163879 |
+ |
MAD1L1 |
NNNGGGAACAGAAACGTGCA |
NGT |
2 |
0.0092 |
Tier II |
| 12 |
NC_000023.11 |
X |
115009761 |
+ |
IL13RA2 |
NNNGGTAACAGAAACCTCCA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000008.11 |
8 |
143041440 |
- |
LY6S-AS1 |
NNNGGTAACAGCAACGTTCA |
NGG |
2 |
0.1754 |
Tier III |
| 14 |
NC_000012.12 |
12 |
73786547 |
- |
LINC02445 |
NNNTGTAACAGAAAAGTGCA |
NGG |
2 |
0.0808 |
Tier III |
| 15 |
NC_000015.10 |
15 |
21294210 |
- |
LOC646214 |
NNNGGTAACAGAAACCTGCA |
NGG |
1 |
0.0 |
Tier III |
| 16 |
NT_187382.1 |
15 |
21351 |
- |
LOC102723432 |
NNNGGTAACAGAAACCTGCA |
NGG |
1 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
32031622 |
- |
Pak2 |
NNNTGTAACAGAAACCTGCA |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000069.6 |
3 |
59259351 |
- |
P2ry12 |
NNNAGCAACAGAAACGTGCA |
NGG |
2 |
0.8182 |
Tier II |
| 3 |
NC_000069.6 |
3 |
59259351 |
- |
Med12l |
NNNAGCAACAGAAACGTGCA |
NGG |
2 |
0.8182 |
Tier II |
| 4 |
NC_000067.6 |
1 |
136331251 |
+ |
Camsap2 |
NNNGGTAACAGGCACGTGCA |
NGG |
2 |
0.152 |
Tier II |
| 5 |
NC_000067.6 |
1 |
60516628 |
- |
Raph1 |
NNNGGTAACAGACACGTGTA |
NGG |
2 |
0.0972 |
Tier II |
| 6 |
NC_000075.6 |
9 |
110420078 |
- |
Ngp |
NNNGGTAAGAGAAACGTGCA |
NTG |
2 |
0.0241 |
Tier II |
| 7 |
NC_000071.6 |
5 |
100098648 |
- |
Tmem150c |
NNNGGTAACAGAAAGGTCCA |
NGG |
2 |
0.0238 |
Tier II |
| 8 |
NC_000079.6 |
13 |
97077699 |
- |
Fam169a |
NNNGGTAACAGAAACCTGCA |
NGG |
1 |
0.0 |
Tier II |
| 9 |
NC_000068.7 |
2 |
167309188 |
+ |
B4galt5 |
NNNGGTAACAGAAACCTGCT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000070.6 |
4 |
10792804 |
+ |
1700123O12Rik |
NNNGGTGACAGAAACGTGAA |
NGG |
2 |
0.3025 |
Tier III |
Other clones with same target sequence:
(none)
