Construct: sgRNA BRDN0001147051
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCAAGGAGCGAATCTGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIB1 (10519)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76951
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
90231483 |
- |
CIB1 |
NNNCAAGGAGCGAATCTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
78288371 |
+ |
SKIC8 |
NNNCAAGGAGCGAATGGGCA |
NGG |
2 |
0.0181 |
Tier I |
| 3 |
NC_000015.10 |
15 |
79071099 |
+ |
RASGRF1 |
NNNCAAGGAGCAAATATGCA |
NGG |
2 |
0.9333 |
Tier II |
| 4 |
NC_000006.12 |
6 |
123941898 |
- |
NKAIN2 |
NNNCAAGGATAGAATCTGCA |
NGG |
2 |
0.6094 |
Tier II |
| 5 |
NC_000006.12 |
6 |
110399698 |
+ |
DDO |
NNNCAGGGAGCTAATCTGCA |
NGG |
2 |
0.2747 |
Tier II |
| 6 |
NC_000001.11 |
1 |
14253318 |
- |
KAZN |
NNNCAAGAAGGGAATCTGCA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000007.14 |
7 |
111852950 |
- |
DOCK4 |
NNNCAAGGAGAGAACCTGCA |
NGG |
2 |
0.2045 |
Tier II |
| 8 |
NC_000007.14 |
7 |
4809592 |
- |
RADIL |
NNNCAAGGAGCGTGTCTGCA |
NGG |
2 |
0.14 |
Tier II |
| 9 |
NC_000019.10 |
19 |
53899038 |
- |
PRKCG |
NNNCAAGGAGCCAATCTGCC |
NGG |
2 |
0.1203 |
Tier II |
| 10 |
NC_000005.10 |
5 |
150235610 |
+ |
CAMK2A |
NNNCAAGGAGCGAATCAGGA |
NGG |
2 |
0.0667 |
Tier II |
| 11 |
NC_000023.11 |
X |
21560063 |
+ |
CNKSR2 |
NNNCAAGGAGGGAAGCTGCA |
NGG |
2 |
0.0125 |
Tier II |
| 12 |
NC_000004.12 |
4 |
173548788 |
- |
HAND2-AS1 |
NNNTAAGGAGCCAATCTGCA |
NGG |
2 |
0.4235 |
Tier III |
| 13 |
NC_000001.11 |
1 |
14253318 |
- |
LOC107985467 |
NNNCAAGAAGGGAATCTGCA |
NGG |
2 |
0.25 |
Tier III |
| 14 |
NC_000004.12 |
4 |
121460913 |
+ |
LOC107986309 |
NNNCCAGGAGTGAATCTGCA |
NGG |
2 |
0.1538 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
80228524 |
- |
Cib1 |
NNNCAAGGAGCGAATCTGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000067.6 |
1 |
172121411 |
+ |
Copa |
NNNCAAGGGGCAAATCTGCA |
NGG |
2 |
0.6222 |
Tier I |
| 3 |
NC_000085.6 |
19 |
6929504 |
+ |
Kcnk4 |
NNNCAAGGAGAGAATCTGCA |
NGG |
1 |
0.75 |
Tier II |
| 4 |
NC_000071.6 |
5 |
142580330 |
- |
Mmd2 |
NNNAGAGGAGCGAATCTGCA |
NGG |
2 |
0.6063 |
Tier II |
| 5 |
NC_000074.6 |
8 |
82402818 |
+ |
Il15 |
NNNCAAGGAGCGAATCAGCT |
NGG |
2 |
0.32 |
Tier II |
| 6 |
NC_000077.6 |
11 |
113572653 |
- |
Slc39a11 |
NNNCAGGGAGCGAATCTGAA |
NGG |
2 |
0.3061 |
Tier II |
| 7 |
NC_000071.6 |
5 |
135488353 |
- |
Hip1 |
NNNCAAGGAGCGAATCTCAA |
NGG |
2 |
0.2041 |
Tier II |
| 8 |
NC_000077.6 |
11 |
60366867 |
- |
Drc3 |
NNNGAAGGAGCTAATCTGCA |
NGG |
2 |
0.1923 |
Tier II |
| 9 |
NC_000073.6 |
7 |
115703155 |
+ |
Sox6 |
NNNCAAGGAGCGAATCTTCA |
NAG |
2 |
0.1728 |
Tier II |
| 10 |
NC_000072.6 |
6 |
29451282 |
- |
Flnc |
NNNGAAGGAGCGACTCTGCA |
NGG |
2 |
0.1071 |
Tier II |
| 11 |
NC_000067.6 |
1 |
164819051 |
- |
Dpt |
NNNCAAGGAGTGAATCTGCA |
NAG |
2 |
0.0798 |
Tier II |
| 12 |
NC_000067.6 |
1 |
65370289 |
+ |
Pth2r |
NNNCAAGGACCGAATCTGCA |
NTG |
2 |
0.0156 |
Tier II |
| 13 |
NC_000085.6 |
19 |
6929504 |
+ |
Gm46646 |
NNNCAAGGAGAGAATCTGCA |
NGG |
1 |
0.75 |
Tier III |
| 14 |
NC_000076.6 |
10 |
66395615 |
+ |
Gm40677 |
NNNCAAGGAGCGAGTCTACA |
NGG |
2 |
0.3231 |
Tier III |
Other clones with same target sequence:
(none)
