Construct: sgRNA BRDN0001147055

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTTGGCCTTTGTTGACACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMX (660)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75655

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 15536381 - BMX NNNTGGCCTTTGTTGACACA NGG 0 1.0 Tier I
2 NC_000002.12 2 206005538 - INO80D NNNTGGCTTTTGTTGACTCA NGG 2 0.4375 Tier I
3 NC_000023.11 X 15536381 - ACE2 NNNTGGCCTTTGTTGACACA NGG 0 1.0 Tier II
4 NC_000008.11 8 142679413 - PSCA NNNTGGCTCTTGTTGACACA NGG 2 0.8077 Tier II
5 NC_000007.14 7 130537914 - COPG2 NNNTGCCCTTTATTGACACA NGG 2 0.6364 Tier II
6 NC_000007.14 7 130542340 - COPG2 NNNTGCCCTTTATTGACACA NGG 2 0.6364 Tier II
7 NC_000006.12 6 15354498 + JARID2 NNNTGTCCTTTGTTAACACA NGG 2 0.6275 Tier II
8 NC_000002.12 2 70669070 + ADD2 NNNTGTCCTATGTTGACACA NGG 2 0.5714 Tier II
9 NC_000021.9 21 32382352 + URB1 NNNTGGAATTTGTTGACACA NGG 2 0.4875 Tier II
10 NC_000012.12 12 80629495 - PTPRQ NNNAGGCCATTGTTGACACA NGG 2 0.4422 Tier II
11 NC_000006.12 6 12021249 + HIVEP1 NNNTGGCCTTGATTGACACA NGG 2 0.3733 Tier II
12 NC_000006.12 6 42111862 - C6orf132 NNNTGGACTTTGTTGACATA NGG 2 0.3462 Tier II
13 NC_000002.12 2 1030436 + SNTG2 NNNTGGCCTGTGATGACACA NGG 2 0.3462 Tier II
14 NC_000010.11 10 74651203 + ADK NNNTGGCCTATTTTGACACA NGG 2 0.3297 Tier II
15 NC_000023.11 X 37410861 + PRRG1 NNNTTGCTTTTGTTGACACA NGG 2 0.2625 Tier II
16 NC_000012.12 12 16522425 + MGST1 NNNGGGCCTTTTTTGACACA NGG 2 0.2404 Tier II
17 NC_000002.12 2 39687773 - TMEM178A NNNAGGCCTGTGTTGACACA NGG 2 0.2381 Tier II
18 NC_000010.11 10 69248897 + HKDC1 NNNTGGCCTTTGTTAACCCA NGG 2 0.1793 Tier II
19 NC_000012.12 12 86493500 + MGAT4C NNNTGCCCTTTGTTGGCACA NGG 2 0.1311 Tier II
20 NC_000009.12 9 121652745 - DAB2IP NNNTGGCATTTGTTGGCACA NGG 2 0.125 Tier II
21 NC_000005.10 5 911711 + TRIP13 NNNTGGCCTGTGTTGGCACA NGG 2 0.0962 Tier II
22 NC_000005.10 5 114400577 - KCNN2 NNNAGGCCTTTGTTGACCCA NGG 2 0.0907 Tier II
23 NC_000006.12 6 124641390 + NKAIN2 NNNTGGCCTTTGTTGGTACA NGG 2 0.0897 Tier II
24 NC_000010.11 10 54609572 - PCDH15 NNNTGGCCTTTGTTGATAGA NGG 2 0.0583 Tier II
25 NC_000003.12 3 13599757 + FBLN2 NNNGGGCCTTTGTTGACACA NGA 2 0.0434 Tier II
26 NC_000001.11 1 85864253 + COL24A1 NNNTGTCCTTTGTTGAGACA NGG 2 0.0392 Tier II
27 NC_000013.11 13 77964481 + EDNRB NNNTGTCCTTTGTTGAGACA NGG 2 0.0392 Tier II
28 NC_000015.10 15 33077676 - FMN1 NNNTGGCCTTTGTAGAGACA NGG 2 0.0364 Tier II
29 NC_000006.12 6 18194420 + KDM1B NNNTGGCCTTTGTTGACTCA NGA 2 0.0347 Tier II
30 NC_000006.12 6 37953474 + ZFAND3 NNNTGGCCTTTCTTGAGACA NGG 2 0.0311 Tier II
31 NC_000008.11 8 55357972 + XKR4 NNNTGCCCTTTGTTGACACA NGC 2 0.0152 Tier II
32 NC_000014.9 14 104777859 - AKT1 NNNTGGCCTGTGTTGACACA NGC 2 0.0111 Tier II
33 NC_000011.10 11 66411078 - NPAS4 NNNTGGCCTGTGTGGACACA NGG 2 0.0 Tier II
34 NC_000006.12 6 63986629 + EYS NNNTGGCTTTTGTGGACACA NGG 2 0.0 Tier II
35 NC_000011.10 11 111532501 - HOATZ NNNTTGCCTTTGTTGTCACA NGG 2 0.0 Tier II
36 NC_000008.11 8 97153837 + LOC101927066 NNNTGGCCCTTGATGACACA NGG 2 0.6391 Tier III
37 NC_000008.11 8 97153837 + LOC105375655 NNNTGGCCCTTGATGACACA NGG 2 0.6391 Tier III
38 NC_000014.9 14 54176159 - LOC105370507 NNNTGGCGATTGTTGACACA NGG 2 0.5969 Tier III
39 NC_000012.12 12 80629495 - LOC105369867 NNNAGGCCATTGTTGACACA NGG 2 0.4422 Tier III
40 NC_000003.12 3 21106349 - LOC105376987 NNNTGGCCTTTGATGACACT NGG 2 0.4154 Tier III
41 NC_000008.11 8 53500916 - LOC124901947 NNNTGGCCTCTGTTGACATA NGG 2 0.2462 Tier III
42 NC_000007.14 7 143986055 + SLC16A1P1 NNNTGGCTTTTGTTGACACA NAG 2 0.2269 Tier III
43 NC_000011.10 11 87441001 - LOC107984361 NNNTGGCCTTTGTTGAAACA NAG 2 0.121 Tier III
44 NC_000002.12 2 112894097 + LOC105373563 NNNTGGTCTTTGTTTACACA NGG 2 0.1161 Tier III
45 NC_000001.11 1 169064546 - LINC00970 NNNTGGCCTTTGTTGACAGG NGG 2 0.0956 Tier III
46 NC_000010.11 10 54609572 - LOC105378311 NNNTGGCCTTTGTTGATAGA NGG 2 0.0583 Tier III
47 NC_000003.12 3 174426429 + LOC105374226 NNNTTGCCTTTGTTTACACA NGG 2 0.0429 Tier III
48 NC_000004.12 4 97391192 - STPG2-AS1 NNNTAGCCTTTGTTGTCACA NGG 2 0.0 Tier III
49 NC_000023.11 X 153086576 + LOC124905225 NNNTGGCCTTTGGGGACACA NGG 2 0.0 Tier III
50 NC_000006.12 6 63986629 + LOC107986608 NNNTGGCTTTTGTGGACACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 164208912 + Bmx NNNTGGCCTTGGTTGACACT NGG 2 0.24 Tier I
2 NC_000075.6 9 42797604 - Grik4 NNNTGGCCTTTGTTGACACA NGG 0 1.0 Tier II
3 NC_000072.6 6 90308447 + Chst13 NNNTGGCCTTTGCTGACACA NGG 1 0.7895 Tier II
4 NC_000079.6 13 57427989 - Spock1 NNNTGACCTTAGTTGACACA NGG 2 0.75 Tier II
5 NC_000075.6 9 120376133 + Myrip NNNTGGTCTTTGCTGACACA NGG 2 0.6414 Tier II
6 NC_000078.6 12 117217576 - Ptprn2 NNNTGGTCTTTGATGACACA NGG 2 0.5625 Tier II
7 NC_000085.6 19 4371322 - Kdm2a NNNTGACCTCTGTTGACACA NGG 2 0.5333 Tier II
8 NC_000075.6 9 21661912 - Smarca4 NNNAGGCTTTTGTTGACACA NGG 2 0.4167 Tier II
9 NC_000083.6 17 71550898 - Spdya NNNTGGCCTTTGCTGACATA NGG 2 0.3644 Tier II
10 NC_000071.6 5 110988693 - Ttc28 NNNTGGCCTCTGTAGACACA NGG 2 0.3302 Tier II
11 NC_000085.6 19 38746832 - Plce1 NNNGGGCCTTTGTTGATACA NGG 2 0.2917 Tier II
12 NC_000082.6 16 97440977 - Bace2 NNNTGGGCTTTGTAGACACA NGG 2 0.2913 Tier II
13 NC_000074.6 8 71281556 + Myo9b NNNTGGCCTTATTTGACACA NGG 2 0.2885 Tier II
14 NC_000075.6 9 31088620 - St14 NNNTGGCCTTTGTTAACACA NAG 2 0.244 Tier II
15 NC_000080.6 14 8126108 + Pxk NNNTGGGCTTTGTTGAAACA NGG 2 0.2196 Tier II
16 NC_000069.6 3 123500497 - Prss12 NNNGTGCCTTTGTTGACACA NGG 2 0.1875 Tier II
17 NC_000079.6 13 17657278 - Sugct NNNTTGCCTTTGTTGACTCA NGG 2 0.15 Tier II
18 NC_000086.7 X 9248506 + Lancl3 NNNTTGGCTTTGTTGACACA NGG 2 0.1412 Tier II
19 NC_000067.6 1 4356065 + Rp1 NNNTGGCCTTTCTTGACACA NAG 2 0.1373 Tier II
20 NC_000081.6 15 22793832 + Cdh18 NNNTGGCCTTTCTTGACACA NAG 2 0.1373 Tier II
21 NC_000074.6 8 84343304 - Cacna1a NNNTGGCCTCTGTTGACACC NGG 2 0.1212 Tier II
22 NC_000083.6 17 69470391 + Akain1 NNNTGGCCTCTGTTGACACC NGG 2 0.1212 Tier II
23 NC_000072.6 6 141641189 - Slco1b2 NNNTGGCCTTTGTAGGCACA NGG 2 0.119 Tier II
24 NC_000077.6 11 60751524 + Top3a NNNTGGCCTGTGTTGGCACA NGG 2 0.0962 Tier II
25 NC_000076.6 10 73984304 + Pcdh15 NNNTGGCCTTTGTTGATAGA NGG 2 0.0583 Tier II
26 NC_000078.6 12 41306850 + Immp2l NNNTGGCTTTTGTTGAGACA NGG 2 0.0515 Tier II
27 NC_000068.7 2 168734454 - Atp9a NNNTGGCCTTTTTTGAGACA NGG 2 0.0226 Tier II
28 NC_000067.6 1 193243890 - Hsd11b1 NNNTGGCCTTTGTTGACAAA NTG 2 0.0167 Tier II
29 NC_000076.6 10 59533449 - Mcu NNNTGGCCGTTGTTGACACA NGT 2 0.01 Tier II
30 NC_000082.6 16 94124432 + Sim2 NNNGGGCCTTTGTGGACACA NGG 2 0.0 Tier II
31 NC_000070.6 4 83244819 + Ttc39b NNNTGGCCTCTGTGGACACA NGG 2 0.0 Tier II
32 NC_000085.6 19 45974628 - Armh3 NNNTGGCCTCTGTGGACACA NGG 2 0.0 Tier II
33 NC_000072.6 6 33424274 - Exoc4 NNNTGGCCTTTGTGGACACA NGG 1 0.0 Tier II
34 NC_000077.6 11 120682365 - Aspscr1 NNNTGGCCTTTGTGGACACA NAG 2 0.0 Tier II
35 NC_000068.7 2 69832133 + Klhl23 NNNTGGCCTTTGTGGACACC NGG 2 0.0 Tier II
36 NC_000083.6 17 74837293 - Ttc27 NNNTGGCCTTTGTGGACACC NGG 2 0.0 Tier II
37 NC_000086.7 X 150626223 - Pfkfb1 NNNTGGCCTTTGTGGACACC NGG 2 0.0 Tier II
38 NC_000079.6 13 11856159 - Ryr2 NNNTGGCCTTTGTGGACACT NGG 2 0.0 Tier II
39 NC_000069.6 3 79140320 - Rapgef2 NNNTGGCCTTTGTGGAGACA NGG 2 0.0 Tier II
40 NC_000069.6 3 53462781 - Nhlrc3 NNNTGGCCTTTGTGGGCACA NGG 2 0.0 Tier II
41 NC_000081.6 15 101414338 + Krt7 NNNTGGCCTTTGTTGACACA NTC 2 0.0 Tier II
42 NC_000067.6 1 194745428 + Plxna2 NNNTGGCCTTTGTTGTCACA NGT 2 0.0 Tier II
43 NC_000081.6 15 95109166 - LOC100043315 NNNTGGCCTTTGCTGACACA NGG 1 0.7895 Tier III
44 NC_000079.6 13 57427989 - Gm33294 NNNTGACCTTAGTTGACACA NGG 2 0.75 Tier III
45 NC_000079.6 13 80994203 + 9330111N05Rik NNNTGGTCTTTGATGACACA NGG 2 0.5625 Tier III
46 NC_000083.6 17 51905103 + Gm20098 NNNTTTCCTTTGTTGACACA NGG 2 0.2 Tier III
47 NC_000072.6 6 140716417 + Gm30215 NNNTGGGCTTTGTTGGCACA NGG 2 0.0905 Tier III
48 NC_000067.6 1 73792948 - 6030407O03Rik NNNTGGTCTTTGTTGAGACA NGG 2 0.0478 Tier III
49 NC_000067.6 1 73792948 - Gm29183 NNNTGGTCTTTGTTGAGACA NGG 2 0.0478 Tier III
50 NC_000070.6 4 125387559 - Gm40247 NNNTGGCCCTTGTTGACACA NTG 2 0.036 Tier III
51 NC_000070.6 4 130279784 - Gm34957 NNNTGGCCTTTTTTGAGACA NGG 2 0.0226 Tier III
52 NC_000086.7 X 50599168 - Firre NNNTGGCCTTTGTGGACACA NGT 2 0.0 Tier III
53 NC_000086.7 X 50613771 - Firre NNNTGGCCTTTGTGGACACA NGT 2 0.0 Tier III
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Other clones with same target sequence:

(none)