Construct: sgRNA BRDN0001147060
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCACCACAGGCCAAAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKG (1608)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77456
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
186257904 |
+ |
DGKG |
NNNCACCACAGGCCAAAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
62824335 |
+ |
KCNH5 |
NNNCACCACAGGTCAAAACG |
NGG |
2 |
0.5353 |
Tier II |
| 3 |
NC_000013.11 |
13 |
43544520 |
+ |
ENOX1 |
NNNCACCAAGGGCCAAAACA |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000015.10 |
15 |
45664323 |
- |
SQOR |
NNNCACAACAGGACAAAACA |
NGG |
2 |
0.2885 |
Tier II |
| 5 |
NC_000016.10 |
16 |
75104179 |
- |
ZNRF1 |
NNNCACCACATGCTAAAACA |
NGG |
2 |
0.2821 |
Tier II |
| 6 |
NC_000019.10 |
19 |
41877659 |
+ |
CD79A |
NNNCCCCACAGGCCAAAATA |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000001.11 |
1 |
230223802 |
+ |
GALNT2 |
NNNAACCACAGGCCAAAACA |
NAG |
2 |
0.2183 |
Tier II |
| 8 |
NC_000011.10 |
11 |
31156118 |
+ |
DCDC1 |
NNNCACCAGAGGCAAAAACA |
NGG |
2 |
0.2167 |
Tier II |
| 9 |
NC_000006.12 |
6 |
119249272 |
+ |
MAN1A1 |
NNNCAGCTCAGGCCAAAACA |
NGG |
2 |
0.2143 |
Tier II |
| 10 |
NC_000006.12 |
6 |
35792740 |
+ |
CLPSL1 |
NNNCCCCACAGTCCAAAACA |
NGG |
2 |
0.1923 |
Tier II |
| 11 |
NC_000009.12 |
9 |
116331128 |
- |
PAPPA |
NNNCACCACAGGAAAAAACA |
NGG |
2 |
0.1346 |
Tier II |
| 12 |
NC_000009.12 |
9 |
35751552 |
- |
RGP1 |
NNNCACCACAGGCCAAAAGA |
NGG |
1 |
0.125 |
Tier II |
| 13 |
NC_000003.12 |
3 |
123651484 |
- |
MYLK |
NNNCACCACAGGTCAAGACA |
NGG |
2 |
0.1235 |
Tier II |
| 14 |
NC_000003.12 |
3 |
53595381 |
+ |
CACNA1D |
NNNCACCAGAGGCCAAACCA |
NGG |
2 |
0.1179 |
Tier II |
| 15 |
NC_000023.11 |
X |
124379944 |
+ |
TENM1 |
NNNCACCACACGCCAAAACA |
NAG |
2 |
0.1111 |
Tier II |
| 16 |
NC_000019.10 |
19 |
48160511 |
- |
LIG1 |
NNNCACCACAGGCCAAAGCC |
NGG |
2 |
0.0909 |
Tier II |
| 17 |
NC_000023.11 |
X |
8613806 |
+ |
ANOS1 |
NNNCACCACAGGCATAAACA |
NGG |
2 |
0.07 |
Tier II |
| 18 |
NC_000003.12 |
3 |
121627529 |
+ |
FBXO40 |
NNNCACCACAGGCCAAAACA |
NGA |
1 |
0.0694 |
Tier II |
| 19 |
NC_000020.11 |
20 |
17260644 |
- |
PCSK2 |
NNNCATCACAGGCCAAAACA |
NGT |
2 |
0.015 |
Tier II |
| 20 |
NC_000008.11 |
8 |
117924622 |
+ |
EXT1 |
NNNCACTACAGGCCAAAACA |
NGT |
2 |
0.0131 |
Tier II |
| 21 |
NC_000002.12 |
2 |
124588922 |
+ |
CNTNAP5 |
NNNCACCACAGGCCAAATCA |
NGC |
2 |
0.0111 |
Tier II |
| 22 |
NC_000004.12 |
4 |
83423015 |
- |
HELQ |
NNNCACCACAGGCCAAAGCA |
NGT |
2 |
0.0065 |
Tier II |
| 23 |
NC_000002.12 |
2 |
42947933 |
- |
LOC112268413 |
NNNCACCACAGTCCAAAGCA |
NGG |
2 |
0.1538 |
Tier III |
| 24 |
NC_000009.12 |
9 |
116331128 |
- |
PAPPA-AS2 |
NNNCACCACAGGAAAAAACA |
NGG |
2 |
0.1346 |
Tier III |
| 25 |
NC_000003.12 |
3 |
123651484 |
- |
LOC124909421 |
NNNCACCACAGGTCAAGACA |
NGG |
2 |
0.1235 |
Tier III |
| 26 |
NC_000006.12 |
6 |
167689883 |
+ |
LINC02487 |
NNNCCCCACAGGCCAGAACA |
NGG |
2 |
0.0962 |
Tier III |
| 27 |
NC_000006.12 |
6 |
167689883 |
+ |
LOC124901465 |
NNNCCCCACAGGCCAGAACA |
NGG |
2 |
0.0962 |
Tier III |
| 28 |
NC_000020.11 |
20 |
17260644 |
- |
LOC105372546 |
NNNCATCACAGGCCAAAACA |
NGT |
2 |
0.015 |
Tier III |
| 29 |
NC_000012.12 |
12 |
82511647 |
- |
LOC107987180 |
NNNCACCACAGGCCAACACA |
NTG |
2 |
0.0069 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
108622634 |
+ |
Olfr507 |
NNNCTCCACAGACCAAAACA |
NGG |
2 |
0.3394 |
Tier I |
| 2 |
NC_000074.6 |
8 |
122242978 |
- |
Zfp469 |
NNNCACCAGAGGCCAAAACA |
NGG |
1 |
0.619 |
Tier II |
| 3 |
NC_000085.6 |
19 |
32661540 |
- |
Papss2 |
NNNCAGCACAGACCAAAACA |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000068.7 |
2 |
28731081 |
+ |
Ak8 |
NNNCACCATAGCCCAAAACA |
NGG |
2 |
0.4632 |
Tier II |
| 5 |
NC_000078.6 |
12 |
31739208 |
+ |
Cog5 |
NNNCACCACAGGCTAAAACT |
NGG |
2 |
0.44 |
Tier II |
| 6 |
NC_000068.7 |
2 |
37911435 |
+ |
Dennd1a |
NNNAAGCACAGGCCAAAACA |
NGG |
2 |
0.4211 |
Tier II |
| 7 |
NC_000075.6 |
9 |
89600378 |
+ |
Minar1 |
NNNCACCACAGGACAAAACA |
NGG |
1 |
0.3846 |
Tier II |
| 8 |
NC_000072.6 |
6 |
71940538 |
- |
Polr1a |
NNNCACCTAAGGCCAAAACA |
NGG |
2 |
0.3673 |
Tier II |
| 9 |
NC_000084.6 |
18 |
7434644 |
+ |
Mpp7 |
NNNGGCCACAGGCCAAAACA |
NGG |
2 |
0.36 |
Tier II |
| 10 |
NC_000071.6 |
5 |
63753611 |
- |
Nwd2 |
NNNCACCACATACCAAAACA |
NGG |
2 |
0.359 |
Tier II |
| 11 |
NC_000083.6 |
17 |
55770380 |
- |
Adgre4 |
NNNCAACACAGGCAAAAACA |
NGG |
2 |
0.325 |
Tier II |
| 12 |
NC_000069.6 |
3 |
68412893 |
- |
Schip1 |
NNNCAACACAGGCCCAAACA |
NGG |
2 |
0.2532 |
Tier II |
| 13 |
NC_000069.6 |
3 |
68412893 |
- |
Iqschfp |
NNNCAACACAGGCCCAAACA |
NGG |
2 |
0.2532 |
Tier II |
| 14 |
NC_000083.6 |
17 |
5897309 |
- |
Snx9 |
NNNCACCACTGGCCCAAACA |
NGG |
2 |
0.2406 |
Tier II |
| 15 |
NC_000074.6 |
8 |
28724475 |
+ |
Unc5d |
NNNCACCCCAGGACAAAACA |
NGG |
2 |
0.1648 |
Tier II |
| 16 |
NC_000079.6 |
13 |
51666331 |
- |
Secisbp2 |
NNNCACCTCAGGACAAAACA |
NGG |
2 |
0.1648 |
Tier II |
| 17 |
NC_000074.6 |
8 |
93108129 |
+ |
Ces1c |
NNNCGCCACAGGCCAAAACC |
NGG |
2 |
0.1636 |
Tier II |
| 18 |
NC_000071.6 |
5 |
23162356 |
- |
Lhfpl3 |
NNNCACCCCCGGCCAAAACA |
NGG |
2 |
0.1429 |
Tier II |
| 19 |
NC_000068.7 |
2 |
30395796 |
- |
Dolpp1 |
NNNCACCATAGGCCAAAAGA |
NGG |
2 |
0.1094 |
Tier II |
| 20 |
NC_000077.6 |
11 |
98121730 |
- |
Fbxl20 |
NNNAACCACAGGCCAAAAGA |
NGG |
2 |
0.1053 |
Tier II |
| 21 |
NC_000077.6 |
11 |
5398702 |
+ |
Znrf3 |
NNNCACCACATGCCCAAACA |
NGG |
2 |
0.1049 |
Tier II |
| 22 |
NC_000070.6 |
4 |
8732361 |
- |
Chd7 |
NNNCAGCACAGGCCAGAACA |
NGG |
2 |
0.0962 |
Tier II |
| 23 |
NC_000070.6 |
4 |
123879259 |
- |
Gm31309 |
NNNCACCACAGTCCAAAACC |
NGG |
2 |
0.0874 |
Tier II |
| 24 |
NC_000074.6 |
8 |
72685744 |
+ |
Nwd1 |
NNNCACCAGAGGCCAAAAGA |
NGG |
2 |
0.0774 |
Tier II |
| 25 |
NC_000075.6 |
9 |
71709994 |
+ |
Cgnl1 |
NNNCACCACAGGCAAAGACA |
NGG |
2 |
0.0618 |
Tier II |
| 26 |
NC_000085.6 |
19 |
29930442 |
- |
Il33 |
NNNCACCAAAGGCCAAAACA |
NGA |
2 |
0.0595 |
Tier II |
| 27 |
NC_000076.6 |
10 |
116146738 |
- |
Ptprr |
NNNAACCACAGGCCAAAACA |
NGA |
2 |
0.0585 |
Tier II |
| 28 |
NC_000071.6 |
5 |
32976696 |
- |
Depdc5 |
NNNCACCACAGGCCAAGCCA |
NGG |
2 |
0.0336 |
Tier II |
| 29 |
NC_000068.7 |
2 |
110069706 |
+ |
Ccdc34 |
NNNCACCACAGGCCAAGACA |
NTG |
2 |
0.0069 |
Tier II |
| 30 |
NC_000069.6 |
3 |
84460543 |
- |
Fhdc1 |
NNNCACCACAGGGCACAACA |
NGG |
2 |
0.0 |
Tier II |
| 31 |
NC_000077.6 |
11 |
35228425 |
- |
Slit3 |
NNNCACCACAGGGGAAAACA |
NGG |
2 |
0.0 |
Tier II |
| 32 |
NC_000068.7 |
2 |
59405403 |
+ |
Gm46743 |
NNNAACCACAGGCCAAATCA |
NGG |
2 |
0.4211 |
Tier III |
| 33 |
NC_000078.6 |
12 |
110208558 |
+ |
Gm40575 |
NNNCACCACAGACCAAAGCA |
NGG |
2 |
0.3733 |
Tier III |
| 34 |
NC_000073.6 |
7 |
108486721 |
+ |
Olfr500-ps1 |
NNNCTCCACAGACCAAAACA |
NGG |
2 |
0.3394 |
Tier III |
| 35 |
NC_000073.6 |
7 |
94247058 |
+ |
Gm32647 |
NNNCACCACTGGCAAAAACA |
NGG |
2 |
0.3088 |
Tier III |
| 36 |
NC_000077.6 |
11 |
18489000 |
- |
Gm12022 |
NNNCACCACAGGCCCAAACA |
NGG |
1 |
0.2727 |
Tier III |
| 37 |
NC_000073.6 |
7 |
108277558 |
+ |
Olfr489-ps1 |
NNNCTCCACAGGCCAGAACA |
NGG |
2 |
0.0699 |
Tier III |
| 38 |
NC_000068.7 |
2 |
159958587 |
- |
4933416E03Rik |
NNNCACCACAGGCAAACACA |
NGG |
2 |
0.0618 |
Tier III |
| 39 |
NC_000077.6 |
11 |
51206455 |
- |
Platr20 |
NNNCACCACAGGCCAAAGCA |
NGA |
2 |
0.0278 |
Tier III |
Other clones with same target sequence:
(none)
