Construct: sgRNA BRDN0001147061

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCAGAGCGTGGGAAAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCD (5580)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76004

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 53179589 + PRKCD NNNAGAGCGTGGGAAAACAC NGG 0 1.0 Tier I
2 NC_000008.11 8 125108344 + NSMCE2 NNNAGAGAGTAGGAAAACAC NGG 2 0.65 Tier II
3 NC_000009.12 9 124622239 - NR6A1 NNNAGAGCGTGGGAAAATAC NGG 1 0.6429 Tier II
4 NC_000001.11 1 110177072 + SLC6A17 NNNAGGGTGTGGGAAAACAC NGG 2 0.625 Tier II
5 NC_000006.12 6 136583532 + MAP3K5 NNNAGTGTGTGGGAAAACAC NGG 2 0.625 Tier II
6 NC_000004.12 4 46846918 + COX7B2 NNNAGAGTGTGGGAAAAAAC NGG 2 0.4712 Tier II
7 NC_000021.9 21 44865057 + PTTG1IP NNNACAGCCTGGGAAAACAC NGG 2 0.4231 Tier II
8 NC_000001.11 1 211479898 - RD3 NNNACAGCTTGGGAAAACAC NGG 2 0.419 Tier II
9 NC_000007.14 7 70600083 - AUTS2 NNNAGAACGTGTGAAAACAC NGG 2 0.3846 Tier II
10 NC_000010.11 10 74500449 + ADK NNNAGGGCTTGGGAAAACAC NGG 2 0.381 Tier II
11 NC_000004.12 4 99347879 - ADH1C NNNCAAGCGTGGGAAAACAC NGG 2 0.3059 Tier II
12 NC_000022.11 22 31897962 - DEPDC5 NNNAGAGCGTGGGAGAACAC NAG 2 0.1685 Tier II
13 NC_000001.11 1 18636410 - PAX7 NNNGGAGCGTGGGAAAACCC NGG 2 0.1293 Tier II
14 NC_000019.10 19 45625765 - EML2 NNNAGAGGGTGGGAAGACAC NGG 2 0.1236 Tier II
15 NC_000007.14 7 105849561 - ATXN7L1 NNNAGAGGGTGGGAAACCAC NGG 2 0.1134 Tier II
16 NC_000022.11 22 38351020 - TPTEP2-CSNK1E NNNAGAGGGTGGGAAAACAC NTG 2 0.025 Tier II
17 NC_000004.12 4 172888600 - GALNTL6 NNNAGAGCGTGGGAACAGAC NGG 2 0.0 Tier II
18 NC_000006.12 6 39975430 + LOC102723789 NNNAGAACTTGGGAAAACAC NGG 2 0.5333 Tier III
19 NC_000021.9 21 44865057 + LOC124905039 NNNACAGCCTGGGAAAACAC NGG 2 0.4231 Tier III
20 NC_000014.9 14 84231810 - LOC105370603 NNNAGCGTGTGGGAAAACAC NGG 2 0.3977 Tier III
21 NC_000009.12 9 1460631 + LOC102723803 NNNAGAGCATGGGGAAACAC NGG 2 0.3 Tier III
22 NC_000008.11 8 127442445 - CASC8 NNNAGAGAGTGGGAAAACGC NGG 2 0.2438 Tier III
23 NC_000009.12 9 36785828 - LOC105376030 NNNAGAGCTTTGGAAAACAC NGG 2 0.2051 Tier III
24 NC_000008.11 8 16891137 + LOC105379297 NNNAGAGCTTGGGAAATCAC NGG 2 0.0711 Tier III
25 NC_000020.11 20 58143315 - LOC107985434 NNNAGAGAGTGGGAAAACAC NGA 2 0.0451 Tier III
26 NC_000022.11 22 38351020 - TPTEP2 NNNAGAGGGTGGGAAAACAC NTG 2 0.025 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 30609290 - Prkcd NNNAGAGCGAGGGAAGACAC NGG 2 0.1648 Tier I
2 NC_000070.6 4 20449633 - Nkain3 NNNAGACCGTGGGAAAACAC NGG 1 0.6875 Tier II
3 NC_000071.6 5 124215877 + Pitpnm2 NNNAGAGCCTGAGAAAACAC NGG 2 0.5026 Tier II
4 NC_000068.7 2 130633846 + Lzts3 NNNAGATCGAGGGAAAACAC NGG 2 0.4898 Tier II
5 NC_000077.6 11 105984740 + Ace NNNAGCACGTGGGAAAACAC NGG 2 0.4545 Tier II
6 NC_000082.6 16 13577120 + Parn NNNAGACCATGGGAAAACAC NGG 2 0.442 Tier II
7 NC_000067.6 1 88096948 - Ugt1a10 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
8 NC_000067.6 1 88096948 - Ugt1a7c NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
9 NC_000067.6 1 88096948 - Ugt1a9 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
10 NC_000067.6 1 88096948 - Ugt1a8 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
11 NC_000067.6 1 88128392 - Ugt1a10 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
12 NC_000067.6 1 88128392 - Ugt1a7c NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
13 NC_000067.6 1 88128392 - Ugt1a9 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
14 NC_000067.6 1 88128392 - Ugt1a6b NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
15 NC_000067.6 1 88128392 - Ugt1a8 NNNTGAGAGTGGGAAAACAC NGG 2 0.4136 Tier II
16 NC_000067.6 1 21456452 + Kcnq5 NNNAGGGCCTGGGAAAACAC NGG 2 0.3846 Tier II
17 NC_000076.6 10 75108534 - Bcr NNNAGAGGGTGGGAAAACAA NGG 2 0.3214 Tier II
18 NC_000074.6 8 47339413 + Stox2 NNNAGAGCATCGGAAAACAC NGG 2 0.2755 Tier II
19 NC_000080.6 14 67865402 - Dock5 NNNAGAGCTGGGGAAAACAC NGG 2 0.2667 Tier II
20 NC_000070.6 4 24567437 - Mms22l NNNAGAGCATTGGAAAACAC NGG 2 0.2473 Tier II
21 NC_000084.6 18 61161491 + Hmgxb3 NNNAAAGCGTGGGAAAACAC NAG 2 0.2247 Tier II
22 NC_000076.6 10 71064433 + Bicc1 NNNAGAGCCTTGGAAAACAC NGG 2 0.2071 Tier II
23 NC_000073.6 7 43799884 - Klk8 NNNAGAGCGTGGGAAAAAGC NGG 2 0.2019 Tier II
24 NC_000069.6 3 86857673 + Dclk2 NNNAGAGAGTGGGAAAACCC NGG 2 0.1345 Tier II
25 NC_000074.6 8 124743868 - Fam89a NNNAGAGGGTGGGAAACCAC NGG 2 0.1134 Tier II
26 NC_000076.6 10 7658848 - Pcmt1 NNNAGGGCGTGGGAAAAGAC NGG 2 0.0952 Tier II
27 NC_000068.7 2 27418790 + Vav2 NNNAGAGCCTGGGAAAGCAC NGG 2 0.095 Tier II
28 NC_000079.6 13 103052433 - Mast4 NNNAGAGAGTGGGAAATCAC NGG 2 0.0867 Tier II
29 NC_000082.6 16 36455189 - Stfa3 NNNAGAGTGTGGGAAAACAC NGA 2 0.0608 Tier II
30 NC_000068.7 2 121565324 - Frmd5 NNNAGACCGTGGGAAAACAC NGA 2 0.0477 Tier II
31 NC_000071.6 5 125141960 - Ncor2 NNNAGACCGTGGGAAAACAC NGA 2 0.0477 Tier II
32 NC_000073.6 7 58714715 - Atp10a NNNAGAGTGTGGGAAAACAC NGT 2 0.0141 Tier II
33 NC_000068.7 2 141053844 + Macrod2 NNNAGACCGTGGGAATACAC NGG 2 0.0 Tier II
34 NC_000068.7 2 3195105 - Fam171a1 NNNAGAGAGTGGGAACACAC NGG 2 0.0 Tier II
35 NC_000080.6 14 32454431 - Chat NNNAGAGGGTGGGAACACAC NGG 2 0.0 Tier II
36 NC_000077.6 11 105984740 + Gm11651 NNNAGCACGTGGGAAAACAC NGG 2 0.4545 Tier III
37 NC_000069.6 3 9179314 + Gm33018 NNNAGACCATGGGAAAACAC NGG 2 0.442 Tier III
38 NC_000073.6 7 58714715 - Gm39019 NNNAGAGTGTGGGAAAACAC NGT 2 0.0141 Tier III
Download CSV

Other clones with same target sequence:

(none)