Construct: sgRNA BRDN0001147064

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTGACTCCTCAGACCCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRM (6723)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76279

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 11056609 - SRM NNNGACTCCTCAGACCCCAT NGG 0 1.0 Tier I
2 NC_000019.10 19 48974678 - GYS1 NNNGACTCCTCAGACCCCAT NCT 2 0.0 Tier I
3 NC_000002.12 2 80178536 + CTNNA2 NNNGACACCTCAGACACCAT NGG 2 0.875 Tier II
4 NC_000005.10 5 153784737 - GRIA1 NNNGACAACTCAGACCCCAT NGG 2 0.5688 Tier II
5 NC_000002.12 2 124249107 - CNTNAP5 NNNGACTCCACAGACCCCTT NGG 2 0.4615 Tier II
6 NC_000009.12 9 2109511 + SMARCA2 NNNGCCTCTTCAGACCCCAT NGG 2 0.4375 Tier II
7 NC_000006.12 6 127337015 - ECHDC1 NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier II
8 NC_000007.14 7 157377303 + DNAJB6 NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier II
9 NC_000012.12 12 39540304 - ABCD2 NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier II
10 NC_000021.9 21 43538990 - HSF2BP NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier II
11 NC_000006.12 6 3319182 + SLC22A23 NNNTGCTCCTCAGACCCCAT NGG 2 0.2618 Tier II
12 NC_000005.10 5 78271714 - AP3B1 NNNGACTCCTCAGGCCCCTT NGG 2 0.2513 Tier II
13 NC_000012.12 12 111176009 - CUX2 NNNGACTCCTCAAACCCCAT NAG 2 0.2393 Tier II
14 NC_000016.10 16 53887438 + FTO NNNGCCTCCTCAGACCACAT NGG 2 0.2333 Tier II
15 NC_000010.11 10 130188534 - LOC124902561 NNNGACTCCTCACACCTCAT NGG 2 0.1965 Tier II
16 NC_000019.10 19 8589139 - ADAMTS10 NNNAACTCCTCAGACCCCAG NGG 2 0.1588 Tier II
17 NC_000002.12 2 134505984 - TMEM163 NNNGCCTCCTCAGACTCCAT NGG 2 0.1538 Tier II
18 NC_000001.11 1 6486887 + PLEKHG5 NNNCACTCCTCAGAACCCAT NGG 2 0.1176 Tier II
19 NC_000017.11 17 46864586 - WNT9B NNNGACTCCTCTGACCCCCT NGG 2 0.069 Tier II
20 NC_000017.11 17 46864586 - LRRC37A2 NNNGACTCCTCTGACCCCCT NGG 2 0.069 Tier II
21 NC_000003.12 3 172197520 + FNDC3B NNNGAATCCTCAGACCCCAT NGA 2 0.0645 Tier II
22 NC_000001.11 1 209854524 + UTP25 NNNGACTCCTCATACCCCAG NGG 2 0.0529 Tier II
23 NC_000007.14 7 104374081 + LHFPL3 NNNGACTCCTCAGACCCCTT NGA 2 0.0374 Tier II
24 NC_000019.10 19 6232336 - MLLT1 NNNGACTCCTCAGTCCCCAT NGA 2 0.037 Tier II
25 NC_000002.12 2 10401340 + HPCAL1 NNNGACTTCTCAGACCCCAT NTG 2 0.0341 Tier II
26 NC_000022.11 22 28000479 - TTC28 NNNGACCCCTCAGACCCCAT NTG 2 0.0268 Tier II
27 NC_000017.11 17 19024548 - GRAP NNNGACTCCCCAGACCCCAT NTG 2 0.0208 Tier II
28 NC_000014.9 14 60919631 - MNAT1 NNNGACTCCTCAGACCCCAC NGC 2 0.002 Tier II
29 NC_000015.10 15 81749170 - LOC124903541 NNNGACAGCTCAGACCCCAT NGG 2 0.5625 Tier III
30 NC_000012.12 12 52217854 + LINC00592 NNNAAGTCCTCAGACCCCAT NGG 2 0.45 Tier III
31 NC_000020.11 20 47860297 + SRMP1 NNNGACTTCTCAGACCACAT NGG 2 0.4083 Tier III
32 NC_000004.12 4 62176677 + LOC101927145 NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier III
33 NC_000006.12 6 127337015 - LOC105377994 NNNGACTCCCCAGACCCCTT NGG 2 0.2872 Tier III
34 NC_000012.12 12 22826890 - LINC02955 NNNGACTCCTCAGGCCCCTT NGG 2 0.2513 Tier III
35 NC_000004.12 4 174842451 + LOC101928551 NNNGACTCCTTAGACCCCTT NGG 2 0.1657 Tier III
36 NC_000019.10 19 56499406 - LOC124904776 NNNGACTCCTTAGACCCCTT NGG 2 0.1657 Tier III
37 NC_000017.11 17 41325842 - TBC1D3P7 NNNGACTCCTCTGGCCCCAT NGG 2 0.1556 Tier III
38 NC_000022.11 22 50633848 - LOC124905149 NNNGACTCCTCAGGCCCCAT NAG 2 0.121 Tier III
39 NC_000017.11 17 35905714 + LRRC37A8P NNNGACTCCTCAGACCCCTT NGA 2 0.0374 Tier III
40 NC_000022.11 22 28000479 - TTC28-AS1 NNNGACCCCTCAGACCCCAT NTG 2 0.0268 Tier III
41 NC_000014.9 14 60919631 - SRMP2 NNNGACTCCTCAGACCCCAC NGC 2 0.002 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 148593437 + Srm NNNGACTCCTCAGACCCCAT NGG 0 1.0 Tier I
2 NC_000072.6 6 90473591 - Klf15 NNNGACTCCTCAGACCACAG NGG 2 0.0824 Tier I
3 NC_000073.6 7 45447954 + Gys1 NNNGACTCCTCAGACCCCAT NTT 2 0.0 Tier I
4 NC_000076.6 10 77137578 + Col18a1 NNNGACTCCTCAGACACCAT NGG 1 1.0 Tier II
5 NC_000084.6 18 65882292 + Grp NNNGACTTCTCAGACCCAAT NGG 2 0.4712 Tier II
6 NC_000078.6 12 16605786 - Lpin1 NNNGACTTCTCAGACTCCAT NGG 2 0.2692 Tier II
7 NC_000074.6 8 11224355 + Col4a1 NNNGACTCCTCAGTCCACAT NGG 2 0.2489 Tier II
8 NC_000073.6 7 49069047 + Nav2 NNNGACTCCTCAGCCCCCAT NGG 1 0.2143 Tier II
9 NC_000077.6 11 80573424 + Myo1d NNNGTGTCCTCAGACCCCAT NGG 2 0.1818 Tier II
10 NC_000083.6 17 69469742 - Akain1 NNNGACTCCTCTGTCCCCAT NGG 2 0.1778 Tier II
11 NC_000075.6 9 48197517 - Nxpe4 NNNGCCTCCTCAGCCCCCAT NGG 2 0.1071 Tier II
12 NC_000068.7 2 6555424 + Celf2 NNNGACTCCTCAGACACCAT NGA 2 0.0694 Tier II
13 NC_000085.6 19 43965840 + Cpn1 NNNGGCTCCTCAGACCCCAT NTG 2 0.0281 Tier II
14 NC_000071.6 5 130336300 - Tyw1 NNNGACTCCTCATACCCCAC NGG 2 0.0273 Tier II
15 NC_000072.6 6 118124138 - Csgalnact2 NNNGAGTCCTCAGAGCCCAT NGG 2 0.025 Tier II
16 NC_000074.6 8 87800850 - Zfp423 NNNGACTCCTCATAGCCCAT NGG 2 0.015 Tier II
17 NC_000070.6 4 155738190 - Tmem240 NNNGACTCCTCAGATCCCCT NGG 2 0.0138 Tier II
18 NC_000068.7 2 128780566 + Mertk NNNGACTCCTGAGAGCCCAT NGG 2 0.0125 Tier II
19 NC_000076.6 10 77137578 + Gm7775 NNNGACTCCTCAGACACCAT NGG 1 1.0 Tier III
20 NC_000075.6 9 43683570 + Gm5364 NNNGACTTCTCAGACCCCAT NGG 1 0.875 Tier III
21 NC_000084.6 18 65882292 + Gm30094 NNNGACTTCTCAGACCCAAT NGG 2 0.4712 Tier III
22 NC_000082.6 16 50606171 + 4930542D17Rik NNNGACTCCTAAGACCCAAT NGG 2 0.4038 Tier III
23 NC_000069.6 3 52476324 + Gm38537 NNNGACTCCTCAGTCCCCTT NGG 2 0.2872 Tier III
24 NC_000073.6 7 130299717 - Gm33555 NNNGACTTCTCAGACTCCAT NGG 2 0.2692 Tier III
25 NC_000073.6 7 49069047 + Gm32849 NNNGACTCCTCAGCCCCCAT NGG 1 0.2143 Tier III
26 NC_000080.6 14 70830718 + Gm34500 NNNGATTCCTCAGACCCCAC NGG 2 0.0844 Tier III
27 NC_000069.6 3 130684581 - Gm36373 NNNGGCTCCTCAGACCCCAT NGC 2 0.016 Tier III
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Other clones with same target sequence:

(none)