Construct: sgRNA BRDN0001147067
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGACTAACCCAGCACGACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFRL1 (53834)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76977
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
1022467 |
- |
FGFRL1 |
NNNCTAACCCAGCACGACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
4037496 |
- |
PIAS4 |
NNNCTCACCCAGCACGAGGA |
NGG |
2 |
0.0606 |
Tier I |
| 3 |
NC_000010.11 |
10 |
89234031 |
+ |
LIPA |
NNNCTAACCCAGCAAGAAGA |
NGG |
2 |
0.1197 |
Tier II |
| 4 |
NC_000016.10 |
16 |
1074424 |
- |
SSTR5 |
NNNCTAACCCAGCACGAGGT |
NGG |
2 |
0.08 |
Tier II |
| 5 |
NC_000007.14 |
7 |
100579682 |
+ |
LRCH4 |
NNNCTAACCCAGCATGAGGA |
NGG |
2 |
0.0089 |
Tier II |
| 6 |
NC_000016.10 |
16 |
1074424 |
- |
SSTR5-AS1 |
NNNCTAACCCAGCACGAGGT |
NGG |
2 |
0.08 |
Tier III |
| 7 |
NC_000021.9 |
21 |
44992690 |
+ |
LINC00163 |
NNNCTAGCCCAGCAGGACGA |
NGG |
2 |
0.0353 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
47666179 |
- |
Col17a1 |
NNNCAAACCCAGCACGAAGA |
NGG |
2 |
0.2692 |
Tier II |
| 2 |
NC_000083.6 |
17 |
73522223 |
+ |
Galnt14 |
NNNTTAACCCAGCACGACGA |
NGA |
2 |
0.0556 |
Tier II |
| 3 |
NC_000085.6 |
19 |
45991369 |
+ |
Armh3 |
NNNCTAACCCAGCACCTCGA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
