Construct: sgRNA BRDN0001147076
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGAGTTCATTGGATGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KSR1 (8844)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77860
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
27582687 |
+ |
KSR1 |
NNNGGAGTTCATTGGATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
44275785 |
+ |
ALX4 |
NNNAGAGCTCATTGGATGCG |
NGG |
2 |
0.9 |
Tier II |
| 3 |
NC_000007.14 |
7 |
3522508 |
+ |
SDK1 |
NNNGGAGTTCCTTGGATGCA |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000008.11 |
8 |
113419029 |
+ |
CSMD3 |
NNNGGAGTTCATTGGATGGG |
NGG |
1 |
0.125 |
Tier II |
| 5 |
NC_000002.12 |
2 |
82927902 |
- |
LOC105374832 |
NNNGGAGTTCATTGAATGAG |
NGG |
2 |
0.4034 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
61835113 |
+ |
Pard3b |
NNNGGAGTTTATTGGAGGCG |
NGG |
2 |
0.1107 |
Tier II |
| 2 |
NC_000070.6 |
4 |
28227678 |
+ |
Gm52725 |
NNNGGATTTCATTGGATGCC |
NGG |
2 |
0.2449 |
Tier III |
| 3 |
NC_000067.6 |
1 |
61835113 |
+ |
Pard3bos1 |
NNNGGAGTTTATTGGAGGCG |
NGG |
2 |
0.1107 |
Tier III |
| 4 |
NC_000070.6 |
4 |
141278603 |
+ |
Gm13056 |
NNNGGAGTTCATTGGCTGCT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
