Construct: sgRNA BRDN0001147077

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGAGGCAGATTATAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFN1 (91156)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76826

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 201211645 + IGFN1 NNNTGAGGCAGATTATAGGA NGG 0 1.0 Tier I
2 NC_000001.11 1 201209701 + IGFN1 NNNTGAAGCAGGTTATAGGA NGG 2 0.7222 Tier I
3 NC_000001.11 1 201210133 + IGFN1 NNNTGAAGCAGGTTATAGGA NGG 2 0.7222 Tier I
4 NC_000001.11 1 201210457 + IGFN1 NNNTGAAGCAGGTTATAGGA NGG 2 0.7222 Tier I
5 NC_000001.11 1 201210025 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
6 NC_000001.11 1 201210241 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
7 NC_000001.11 1 201210349 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
8 NC_000001.11 1 201210565 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
9 NC_000001.11 1 201210673 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
10 NC_000001.11 1 201210889 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
11 NC_000001.11 1 201210997 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
12 NC_000001.11 1 201211105 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
13 NC_000001.11 1 201211213 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
14 NC_000001.11 1 201211321 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
15 NC_000001.11 1 201211429 + IGFN1 NNNTGAGGCAGGTTATAGGA NGG 1 0.7222 Tier I
16 NC_000001.11 1 201209593 + IGFN1 NNNTAAGGCAGGTTATAGGA NGG 2 0.6259 Tier I
17 NC_000001.11 1 201209809 + IGFN1 NNNTAAGGCAGGTTATAGGA NGG 2 0.6259 Tier I
18 NC_000001.11 1 201210781 + IGFN1 NNNTAAGGCAGGTTATAGGA NGG 2 0.6259 Tier I
19 NC_000001.11 1 201211537 + IGFN1 NNNTAAGGCAGGTTATAGGA NGG 2 0.6259 Tier I
20 NC_000001.11 1 201209917 + IGFN1 NNNTGAGGCAGGTTATAGGA NGA 2 0.0502 Tier I
21 NC_000007.14 7 22294593 - RAPGEF5 NNNTGAGGAAGACTATAGGA NGG 2 0.6767 Tier II
22 NC_000001.11 1 156244650 - PAQR6 NNNGGAGGCAAATTATAGGA NGG 2 0.625 Tier II
23 NC_000003.12 3 85237203 + CADM2 NNNTGAGACAGATAATAGGA NGG 2 0.619 Tier II
24 NC_000003.12 3 45873825 - LZTFL1 NNNTGGGGAAGATTATAGGA NGG 2 0.6122 Tier II
25 NC_000019.10 19 17748337 - FCHO1 NNNTGGGGCAGATTAGAGGA NGG 2 0.2473 Tier II
26 NC_000006.12 6 100602611 + ASCC3 NNNTGAGGCAGATTAAAGGA NAG 2 0.2357 Tier II
27 NC_000023.11 X 152249545 - GABRA3 NNNGGAGGCAGATTAGAGGA NGG 2 0.2163 Tier II
28 NC_000003.12 3 48529559 - PFKFB4 NNNTGAGGCAGGTTATAGGA NAG 2 0.1872 Tier II
29 NC_000004.12 4 70832116 - GRSF1 NNNTCAGGCAGATTTTAGGA NGG 2 0.1571 Tier II
30 NC_000001.11 1 231813794 - DISC1 NNNTGAGGCAGATTTCAGGA NGG 2 0.1333 Tier II
31 NC_000004.12 4 184313493 - MYL12BP2 NNNAGAGGCAGATTCTAGGA NGG 2 0.1299 Tier II
32 NC_000006.12 6 13215457 + PHACTR1 NNNTTAGGCAGATTAGAGGA NGG 2 0.1038 Tier II
33 NC_000012.12 12 71845317 + TBC1D15 NNNTTAGGCAGATTATTGGA NGG 2 0.04 Tier II
34 NC_000005.10 5 59539048 - PDE4D NNNTGAGGCAGAATATAGGA NGT 2 0.0112 Tier II
35 NC_000007.14 7 95911361 + DYNC1I1 NNNTGAGGCAGATTATTGGA NTG 2 0.0052 Tier II
36 NC_000002.12 2 229175961 - PID1 NNNTGAGGCAGATTATGGGA NGC 2 0.0039 Tier II
37 NC_000003.12 3 1206250 - CNTN6 NNNTGAGACAGATGATAGGA NGG 2 0.0 Tier II
38 NC_000012.12 12 8985972 + KLRG1 NNNTGAGGCAGATGAAAGGA NGG 2 0.0 Tier II
39 NC_000020.11 20 32000912 - XKR7 NNNTGAGGCAGATGACAGGA NGG 2 0.0 Tier II
40 NC_000013.11 13 73648549 + LINC00393 NNNGGAGGCAGAATATAGGA NGG 2 0.4327 Tier III
41 NC_000019.10 19 17748337 - LOC124904649 NNNTGGGGCAGATTAGAGGA NGG 2 0.2473 Tier III
42 NC_000006.12 6 43911841 - LINC01512 NNNGGAGGCAGATCATAGGA NGG 2 0.1786 Tier III
43 NC_000013.11 13 89072591 + LOC105370307 NNNTGAGACAGATTATTGGA NGG 2 0.1333 Tier III
44 NC_000001.11 1 231813794 - TSNAX-DISC1 NNNTGAGGCAGATTTCAGGA NGG 2 0.1333 Tier III
45 NC_000001.11 1 231813794 - LOC105373170 NNNTGAGGCAGATTTCAGGA NGG 2 0.1333 Tier III
46 NC_000011.10 11 38308077 - LOC105376634 NNNTGAGGCAGGTTATAGGA NGC 2 0.016 Tier III
47 NC_000005.10 5 117846508 - LINC02147 NNNTAAGGCAGATTATAGGA NGT 2 0.014 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 15714168 - Malrd1 NNNTGAGGCTGATTATAGGA NGG 1 0.8824 Tier II
2 NC_000070.6 4 35990203 + Lingo2 NNNTAAGGCAGATTAAAGGA NGG 2 0.7879 Tier II
3 NC_000079.6 13 77837449 - Fam172a NNNGGAGGCAGATTATAGGA NGG 1 0.625 Tier II
4 NC_000067.6 1 21021753 - Tram2 NNNCGAGGCTGATTATAGGA NGG 2 0.5709 Tier II
5 NC_000067.6 1 8956083 - Sntg1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
6 NC_000067.6 1 11944034 + A830018L16Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
7 NC_000067.6 1 32306806 + Khdrbs2 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
8 NC_000067.6 1 99921390 + Cntnap5b NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
9 NC_000067.6 1 115756309 + Cntnap5a NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
10 NC_000067.6 1 117791511 + B020011L13Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
11 NC_000067.6 1 188282153 + Ush2a NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
12 NC_000068.7 2 113661766 - Fmn1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
13 NC_000068.7 2 139536539 - Sptlc3 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
14 NC_000069.6 3 24357721 + Naaladl2 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
15 NC_000069.6 3 29542931 + Egfem1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
16 NC_000069.6 3 63362682 + Mme NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
17 NC_000069.6 3 75265819 - Serpini2 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
18 NC_000070.6 4 111017130 - Agbl4 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
19 NC_000073.6 7 41843900 - Vmn2r58 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
20 NC_000073.6 7 42184948 - Vmn2r60 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
21 NC_000073.6 7 56447305 + Oca2 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
22 NC_000073.6 7 76300328 + Agbl1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
23 NC_000073.6 7 97890374 + Pak1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
24 NC_000073.6 7 133833730 + Fank1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
25 NC_000076.6 10 88249627 + Washc3 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
26 NC_000077.6 11 11605120 - 4930512M02Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
27 NC_000078.6 12 89157798 + Nrxn3 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
28 NC_000079.6 13 96441834 - Ankdd1b NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
29 NC_000079.6 13 109017310 + Pde4d NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
30 NC_000080.6 14 56442481 + Rnf17 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
31 NC_000081.6 15 48061678 - Csmd3 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
32 NC_000082.6 16 4525068 + Srl NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
33 NC_000082.6 16 28535607 + Fgf12 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
34 NC_000082.6 16 36284679 + Stfa1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
35 NC_000082.6 16 36360076 - Cstdc5 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
36 NC_000083.6 17 40524921 + Esp8 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
37 NC_000083.6 17 53971001 + Sult1c1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
38 NC_000085.6 19 50495912 - Sorcs1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
39 NC_000086.7 X 42734941 - Tenm1 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
40 NC_000086.7 X 101197071 - Snx12 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
41 NC_000086.7 X 112502361 - 2010106E10Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
42 NC_000086.7 X 167558765 + Frmpd4 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier II
43 NC_000076.6 10 3986459 + Mthfd1l NNNAGAGGCAAATTATAGGA NGG 2 0.4762 Tier II
44 NC_000076.6 10 5464073 - Syne1 NNNAGAGGCTGATTATAGGA NGG 2 0.4202 Tier II
45 NC_000076.6 10 28690619 + Themis NNNAGAGGCTGATTATAGGA NGG 2 0.4202 Tier II
46 NC_000078.6 12 53048805 + Akap6 NNNAGAGGCTGATTATAGGA NGG 2 0.4202 Tier II
47 NC_000074.6 8 77370767 - Arhgap10 NNNTGAGGCTGATTATAGGA NAG 2 0.2288 Tier II
48 NC_000084.6 18 35856317 + Cxxc5 NNNTGAGACAGATTTTAGGA NGG 2 0.2 Tier II
49 NC_000082.6 16 90261502 + Scaf4 NNNTGAGGCAGAGTATAGAA NGG 2 0.1863 Tier II
50 NC_000085.6 19 30718388 - Prkg1 NNNTGAGGCAGAGTATAAGA NGG 2 0.1806 Tier II
51 NC_000072.6 6 134088580 + Etv6 NNNTGAGGTAGATTTTAGGA NGG 2 0.175 Tier II
52 NC_000067.6 1 119729538 + Ptpn4 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
53 NC_000068.7 2 96745447 + Lrrc4c NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
54 NC_000069.6 3 118687180 - Dpyd NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
55 NC_000069.6 3 157004799 + Negr1 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
56 NC_000070.6 4 3599799 + Tgs1 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
57 NC_000070.6 4 19831985 + Slc7a13 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
58 NC_000070.6 4 127991914 + Csmd2 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
59 NC_000072.6 6 63320861 + Grid2 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
60 NC_000081.6 15 25938039 - Retreg1 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
61 NC_000085.6 19 39854474 + Cyp2c69 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
62 NC_000086.7 X 130000076 + Diaph2 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
63 NC_000086.7 X 136632962 + Tceal3 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
64 NC_000086.7 X 164105589 + Cltrn NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier II
65 NC_000067.6 1 69601941 + Ikzf2 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
66 NC_000067.6 1 123679092 + Dpp10 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
67 NC_000068.7 2 15689617 + Malrd1 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
68 NC_000068.7 2 22379809 - Myo3a NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
69 NC_000070.6 4 84438712 - Bnc2 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
70 NC_000073.6 7 60177959 - Snrpn NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
71 NC_000076.6 10 63751177 + Ctnna3 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
72 NC_000082.6 16 13301308 - Mrtfb NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier II
73 NC_000080.6 14 31624122 - Hacl1 NNNTGAGGCAGAGTTTAGGA NGG 2 0.0522 Tier II
74 NC_000081.6 15 37501384 - Ncald NNNTGAGGCAGATTATAGCA NTG 2 0.0175 Tier II
75 NC_000078.6 12 54036282 - Npas3 NNNTGAGGCAGGTTATAGGA NGC 2 0.016 Tier II
76 NC_000084.6 18 39122885 + Arhgap26 NNNTGAGGCAGTTTATAGGA NGC 2 0.0074 Tier II
77 NC_000079.6 13 32217837 - Gmds NNNTGAGGCAGATTATCGGA NGT 2 0.0028 Tier II
78 NC_000073.6 7 35460142 + Slc7a9 NNNTGAGGCAGATGACAGGA NGG 2 0.0 Tier II
79 NC_000076.6 10 90207204 - Anks1b NNNTGAGGCAGATTATAGGA NCT 2 0.0 Tier II
80 NC_000067.6 1 73423895 - D530049I02Rik NNNTGAGGCTGATTATAGGA NGG 1 0.8824 Tier III
81 NC_000072.6 6 67979392 - Igk NNNTGTGGCTGATTATAGGA NGG 2 0.6303 Tier III
82 NC_000067.6 1 102284707 + Gm3116 NNNGGAGGCAGATTATAGGA NGG 1 0.625 Tier III
83 NC_000067.6 1 7369537 - Gm26901 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
84 NC_000067.6 1 77834442 - Gm33030 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
85 NC_000067.6 1 77834442 - Gm33083 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
86 NC_000067.6 1 103335249 + Gm18728 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
87 NC_000068.7 2 113940705 - Gm39909 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
88 NC_000069.6 3 45323622 - 2610316D01Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
89 NC_000069.6 3 64749970 - Vmn2r-ps11 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
90 NC_000069.6 3 64766237 - Vmn2r-ps11 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
91 NC_000069.6 3 73522684 + Gm20754 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
92 NC_000071.6 5 42087759 + Gm16223 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
93 NC_000072.6 6 67987326 + Igk NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
94 NC_000072.6 6 68205669 + Igk NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
95 NC_000074.6 8 41974174 + 2810404M03Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
96 NC_000077.6 11 7260040 - Gm36565 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
97 NC_000079.6 13 88524456 + Gm34585 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
98 NC_000084.6 18 29413959 + 4930578E11Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
99 NC_000085.6 19 39249359 + Cyp2c53-ps NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
100 NC_000086.7 X 81535900 - LOC115489112 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
101 NC_000086.7 X 81667978 - LOC115489112 NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
102 NC_000086.7 X 152395272 + 3010001F23Rik NNNGGAGGCTGATTATAGGA NGG 2 0.5515 Tier III
103 NC_000069.6 3 17437291 - Gm30340 NNNAGAGGCTGATTATAGGA NGG 2 0.4202 Tier III
104 NC_000086.7 X 156014068 - Gm15155 NNNAGAGGCTGATTATAGGA NGG 2 0.4202 Tier III
105 NC_000077.6 11 119012068 + Gm35829 NNNTGAGGCAGACTATAGGC NGG 2 0.1794 Tier III
106 NC_000077.6 11 99273154 - Gm32385 NNNTGAGGTAGATTTTAGGA NGG 2 0.175 Tier III
107 NC_000081.6 15 55934572 + Gm46516 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier III
108 NC_000084.6 18 47564816 - Gm5095 NNNTGAGGCTGATTATGGGA NGG 2 0.1557 Tier III
109 NC_000072.6 6 132197974 - Gm18912 NNNCGAGGCAGATTATGGGA NGG 2 0.1142 Tier III
110 NC_000069.6 3 59525351 + Gm4856 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier III
111 NC_000072.6 6 132283403 - Gm43979 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier III
112 NC_000073.6 7 60177959 - Snhg14 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier III
113 NC_000080.6 14 90177535 + 4930474H20Rik NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier III
114 NC_000084.6 18 39818003 + Gm41707 NNNGGAGGCAGATTATGGGA NGG 2 0.1103 Tier III
115 NC_000067.6 1 60783347 + 2310016D23Rik NNNGGAGGCAGATTATTGGA NGG 2 0.0833 Tier III
116 NC_000073.6 7 61964667 - A330076H08Rik NNNTGAGGCTGATTATAGGA NGT 2 0.0142 Tier III
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Other clones with same target sequence:

(none)