Construct: sgRNA BRDN0001147078
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCAGCGAAAGCGCCTTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP2K1 (5604)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000015.10 | 15 | 66435095 | + | MAP2K1 | NNNGCAGCGAAAGCGCCTTG | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000017.11 | 17 | 75610939 | + | MYO15B | NNNGCAGCGAAAGGGCCTTG | NAG | 2 | 0.0 | Tier I |
| 3 | NC_000008.11 | 8 | 30027865 | + | MAP2K1P1 | NNNGCAGCGAAAGCACCTTG | NGG | 1 | 0.9412 | Tier III |
| 4 | NC_000014.9 | 14 | 45466139 | - | LOC105370476 | NNNGCAGCTAAAGCGCCTTA | NGG | 2 | 0.5 | Tier III |
| 5 | NC_000007.14 | 7 | 91387776 | + | LINC02932 | NNNGCAGCGAAAGTGCCTGG | NGG | 2 | 0.1833 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
No results found.