Construct: sgRNA BRDN0001147080
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGTAACCCCGAGCGCTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPPK (64768)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77153
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
92635294 |
+ |
IPPK |
NNNTAACCCCGAGCGCTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
241802516 |
+ |
GAL3ST2 |
NNNTCACCCTGAGCGCTCTG |
NGG |
2 |
0.4706 |
Tier II |
| 3 |
NC_000016.10 |
16 |
81250989 |
+ |
BCO1 |
NNNTGACCCCGAGCCCTCTG |
NGG |
2 |
0.1964 |
Tier II |
| 4 |
NC_000015.10 |
15 |
41941738 |
+ |
EHD4 |
NNNTAGCCCCGAGCTCTCTG |
NGG |
2 |
0.102 |
Tier II |
| 5 |
NC_000021.9 |
21 |
6859008 |
- |
LOC110091777 |
NNNTAACCCCGGGCGCTCTG |
NGG |
1 |
0.7222 |
Tier III |
| 6 |
NC_000021.9 |
21 |
13724026 |
- |
LOC112268283 |
NNNTAACCCCGGGCGCTCTG |
NGG |
1 |
0.7222 |
Tier III |
| 7 |
NT_167208.1 |
|
25532 |
+ |
LOC101927555 |
NNNTAACCCCGGGCGCTCTG |
NGG |
1 |
0.7222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
49449252 |
- |
Ippk |
NNNTAACCCCGAGTGCTCTG |
NGG |
1 |
0.7333 |
Tier I |
| 2 |
NC_000076.6 |
10 |
50760388 |
- |
Ascc3 |
NNNTAACCCAGAGCACTCTG |
NGG |
2 |
0.8157 |
Tier II |
| 3 |
NC_000083.6 |
17 |
6765268 |
- |
Ezr |
NNNTAACCCCCAGCACTCTG |
NGG |
2 |
0.4034 |
Tier II |
| 4 |
NC_000078.6 |
12 |
71585697 |
+ |
9630002D21Rik |
NNNTAACCCCAAGAGCTCTG |
NGG |
2 |
0.35 |
Tier III |
Other clones with same target sequence:
(none)
