Construct: sgRNA BRDN0001147090
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTCCCGTGGATGATTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRBP2 (340371)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76454
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
143839385 |
+ |
NRBP2 |
NNNTCCCGTGGATGATTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
27435157 |
- |
NRBP1 |
NNNTCCCATGGATGATGGGG |
NGG |
2 |
0.1176 |
Tier I |
| 3 |
NC_000008.11 |
8 |
47887212 |
- |
PRKDC |
NNNTCACATGGATGATTGGG |
NGG |
2 |
0.9286 |
Tier II |
| 4 |
NC_000023.11 |
X |
111334291 |
- |
DCX |
NNNTCTCTTGGATGATTGGG |
NGG |
2 |
0.5804 |
Tier II |
| 5 |
NC_000013.11 |
13 |
101926048 |
- |
FGF14 |
NNNTCCCCTGGATTATTGGG |
NGG |
2 |
0.1641 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
56227816 |
+ |
Peak1 |
NNNTGCCGTGGATGCTTGGG |
NGG |
2 |
0.1636 |
Tier I |
| 2 |
NC_000081.6 |
15 |
76090123 |
+ |
Nrbp2 |
NNNTCCCGTGGATGATGGGG |
NGA |
2 |
0.0082 |
Tier I |
| 3 |
NC_000076.6 |
10 |
101847615 |
+ |
Mgat4c |
NNNTCCCATGGATGATTGAG |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000071.6 |
5 |
36911290 |
- |
Ppp2r2c |
NNNTCCCCTGGATGATTGGC |
NGG |
2 |
0.2637 |
Tier II |
| 5 |
NC_000067.6 |
1 |
167807132 |
+ |
Lmx1a |
NNNTCCCGTGAATGATTGGG |
NAG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000072.6 |
6 |
70814198 |
- |
Gm30331 |
NNNTCCCGTGGATGTTTGGG |
NGG |
1 |
0.2 |
Tier III |
Other clones with same target sequence:
(none)
