Construct: sgRNA BRDN0001147091

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTATGTTGCTGGTTAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK1 (53354)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76558

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 89599321 - PANK1 NNNATGTTGCTGGTTAACAT NGG 0 1.0 Tier I
2 NC_000014.9 14 23273296 - HOMEZ NNNATATTGCTCGTTAACAT NGG 2 0.5294 Tier II
3 NC_000014.9 14 23273296 - RNF212B NNNATATTGCTCGTTAACAT NGG 2 0.5294 Tier II
4 NC_000002.12 2 69207015 - ANTXR1 NNNATGTTGCTGGATAAAAT NGG 2 0.3333 Tier II
5 NC_000010.11 10 69935547 - COL13A1 NNNATGGTGCTGTTTAACAT NGG 2 0.1765 Tier II
6 NC_000017.11 17 30469509 - CPD NNNATGATGCTGGTTACCAT NGG 2 0.1544 Tier II
7 NC_000007.14 7 96308188 - SLC25A13 NNNATGCTGCTGGTTAACAG NGG 2 0.1213 Tier II
8 NC_000001.11 1 237185871 - RYR2 NNNATGTTGCTGTTTAACCT NGG 2 0.0621 Tier II
9 NC_000018.10 18 50577558 - MAPK4 NNNATTTTGCTGGTTAACAC NGG 2 0.0606 Tier II
10 NC_000001.11 1 42497415 - CCDC30 NNNATGTTGCTGTTTAGCAT NGG 2 0.0529 Tier II
11 NC_000004.12 4 118060889 + NDST3 NNNATGTTGCTGTTTAACAC NGG 2 0.0273 Tier II
12 NC_000002.12 2 69546825 + AAK1 NNNATGTTACTGGTTAACAT NTG 2 0.025 Tier II
13 NC_000010.11 10 20090329 - PLXDC2 NNNATGTTGCTGGTGAACAT NAG 2 0.013 Tier II
14 NC_000006.12 6 34610217 - ILRUN NNNATGTTGCTAGTTTACAT NGG 2 0.0 Tier II
15 NC_000002.12 2 69207015 - LOC124906019 NNNATGTTGCTGGATAAAAT NGG 2 0.3333 Tier III
16 NC_000004.12 4 118060889 + LOC107986307 NNNATGTTGCTGTTTAACAC NGG 2 0.0273 Tier III
17 NC_000002.12 2 68337525 - LOC102724389 NNNATGTTGCTGGTTAAAAT NTG 2 0.021 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 34827189 - Pank1 NNNATGTTGCTGGTTAACAT NGG 0 1.0 Tier I
2 NC_000081.6 15 58796741 - Tmem65 NNNATATTGCAGGTTAACAT NGG 2 0.75 Tier II
3 NC_000070.6 4 134258771 - Grrp1 NNNACGTTGCTGGTTAATAT NGG 2 0.6429 Tier II
4 NC_000083.6 17 74786164 + Ttc27 NNNATTTTACTGGTTAACAT NGG 2 0.4286 Tier II
5 NC_000075.6 9 110587865 + Setd2 NNNATCTTGCTGGTTAACAA NGG 2 0.3835 Tier II
6 NC_000069.6 3 76375043 + Fstl5 NNNATGTTACTGGTTAAAAT NGG 2 0.3462 Tier II
7 NC_000084.6 18 67883713 + Cep192 NNNATGTTGCTCTTTAACAT NGG 2 0.1588 Tier II
8 NC_000068.7 2 132290192 - Cds2 NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier II
9 NC_000083.6 17 24425738 + Rnps1 NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier II
10 NC_000086.7 X 157944430 + Cnksr2 NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier II
11 NC_000067.6 1 34467759 - Ptpn18 NNNATGTGGCTGGTTACCAT NGG 2 0.1294 Tier II
12 NC_000083.6 17 8628159 + Pde10a NNNATTTTGCTGGTTGACAT NGG 2 0.1282 Tier II
13 NC_000082.6 16 63682113 - Epha3 NNNATTTTGCTGGTTAACAG NGG 2 0.1176 Tier II
14 NC_000071.6 5 127898394 - Tmem132d NNNATGATGCTGGTTAAGAT NGG 2 0.1167 Tier II
15 NC_000072.6 6 71822056 + Mrpl35 NNNATGTTCCTGGTTAACCT NGG 2 0.1114 Tier II
16 NC_000078.6 12 89910231 - Nrxn3 NNNATGTTGCTGGTTAAAAC NGG 2 0.049 Tier II
17 NC_000074.6 8 9277201 + Fam155a NNNATGTTGCTGGTCATCAT NGG 2 0.0364 Tier II
18 NC_000082.6 16 40308169 + Lsamp NNNATGTTGCTGTTTAACAT NGT 2 0.0048 Tier II
19 NC_000083.6 17 7072487 + Gm1604b NNNATGTTGCTGGGTTACAT NGG 2 0.0 Tier II
20 NC_000070.6 4 134258771 - Gm42325 NNNACGTTGCTGGTTAATAT NGG 2 0.6429 Tier III
21 NC_000081.6 15 66299591 + Gm27242 NNNCTGTAGCTGGTTAACAT NGG 2 0.2824 Tier III
22 NC_000069.6 3 122221851 + Gm46822 NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier III
23 NC_000072.6 6 7981986 - Rnps1-ps NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier III
24 NC_000072.6 6 7981986 - Gm35446 NNNATGTTGCAGGTTAACCT NGG 2 0.1552 Tier III
25 NC_000072.6 6 132258012 + Gm43979 NNNATGTTTCTGGTTAACAT NCG 2 0.0571 Tier III
26 NC_000072.6 6 132292556 - Gm43979 NNNATGTTTCTGGTTAACAT NCG 2 0.0571 Tier III
27 NC_000083.6 17 8053650 - Gm1604a NNNATGTTGCTGGGTTACAT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)