Construct: sgRNA BRDN0001147097

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATCCACCCATCCTTCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP4 (23677)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78090

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 235042235 + SH3BP4 NNNCCACCCATCCTTCAAGA NGG 0 1.0 Tier I
2 NC_000008.11 8 99442631 + VPS13B NNNCGACCCATCCTTGAAGA NGG 2 0.0923 Tier I
3 NC_000001.11 1 151138108 - SEMA6C NNNCCACCCATCCCTCCAGA NGG 2 0.0504 Tier I
4 NC_000001.11 1 49041958 + AGBL4 NNNCCACCCATCTTTCAAGA NGG 1 0.7 Tier II
5 NC_000011.10 11 106845079 + GUCY1A2 NNNCCACCCATCTTTCAAGA NGG 1 0.7 Tier II
6 NC_000001.11 1 151827279 - RORC NNNCCTCCCATCCTTCAAGT NGG 2 0.4286 Tier II
7 NC_000023.11 X 31181322 + DMD NNNCCAGCCATCTTTCAAGA NGG 2 0.3294 Tier II
8 NC_000006.12 6 168611847 + SMOC2 NNNCCACACATCCTTCAGGA NGG 2 0.26 Tier II
9 NC_000007.14 7 76268166 + SRRM3 NNNCCACCCTTCCTCCAAGA NGG 2 0.2406 Tier II
10 NC_000009.12 9 113969253 - ZNF618 NNNCCACCCATCCTTCCAGG NGG 2 0.1349 Tier II
11 NC_000010.11 10 70562295 - PALD1 NNNCCACCCATCCATCCAGA NGG 2 0.1092 Tier II
12 NC_000004.12 4 38793152 - TLR1 NNNCCACCCATCCTTCTTGA NGG 2 0.0667 Tier II
13 NC_000007.14 7 70458135 + AUTS2 NNNCCACCCCTCCTTCTAGA NGG 2 0.0444 Tier II
14 NC_000002.12 2 88739034 - RPIA NNNCCACCCATCCTTCACGC NGG 2 0.0433 Tier II
15 NC_000009.12 9 127262539 + GARNL3 NNNCCACACATCCTGCAAGA NGG 2 0.0325 Tier II
16 NC_000011.10 11 19684824 + NAV2 NNNTCACCCATCCTTCAAGA NTG 2 0.0312 Tier II
17 NC_000023.11 X 33053845 - DMD NNNCCACCCATCCCTCAAGA NCG 2 0.0306 Tier II
18 NC_000020.11 20 11907684 - BTBD3 NNNCCACCCATCCTTCAAGA NGC 1 0.0222 Tier II
19 NC_000012.12 12 3447537 + PRMT8 NNNCCACTCATCCTTCAAGA NGC 2 0.0194 Tier II
20 NC_000001.11 1 231897155 - DISC1 NNNCCACTCATCCTTCAAGA NGT 2 0.0141 Tier II
21 NC_000008.11 8 67709776 - CPA6 NNNCCACCCATACTTCAAGA NGT 2 0.0115 Tier II
22 NC_000013.11 13 77579130 + SCEL NNNCAACCCATCCTTCAAGA NGT 2 0.0092 Tier II
23 NC_000006.12 6 142899299 + HIVEP2 NNNCCACCCATCCTGCAAGA NGC 2 0.0011 Tier II
24 NC_000004.12 4 25773892 + SEL1L3 NNNCCACCCATCCTTCAAGA NCA 2 0.0 Tier II
25 NC_000001.11 1 112716148 + PPM1J-DT NNNACACCAATCCTTCAAGA NGG 2 0.7218 Tier III
26 NC_000002.12 2 236940366 - COPS8-DT NNNCCGCCCATCCTTAAAGA NGG 2 0.7143 Tier III
27 NC_000001.11 1 49041958 + LOC105378706 NNNCCACCCATCTTTCAAGA NGG 1 0.7 Tier III
28 NC_000006.12 6 43868975 - LOC105375068 NNNCCATCCATCCTTCAAGG NGG 2 0.6213 Tier III
29 NC_000006.12 6 43868975 - LOC105375070 NNNCCATCCATCCTTCAAGG NGG 2 0.6213 Tier III
30 NC_000002.12 2 122232039 + LOC105373592 NNNCCATCCATCCATCAAGA NGG 2 0.503 Tier III
31 NC_000019.10 19 57065746 + RPL7AP69 NNNCCACCCATCCTTCAAGC NGG 1 0.2273 Tier III
32 NC_000006.12 6 156281203 + LOC101928923 NNNCCACCCATCCTTCAAGG NAG 2 0.1983 Tier III
33 NC_000010.11 10 91684599 + LOC107984253 NNNCCACCCCTCCTTGAAGA NGG 2 0.0513 Tier III
34 NC_000005.10 5 74951027 + LOC105379040 NNNCCACCCATCTTTCAAGA NTG 2 0.0273 Tier III
35 NC_000010.11 10 62906524 - LOC107984012 NNNCCACCCATCCTTCAAGT NTG 2 0.0234 Tier III
36 NC_000012.12 12 3447537 + LOC124902862 NNNCCACTCATCCTTCAAGA NGC 2 0.0194 Tier III
37 NC_000001.11 1 231897155 - TSNAX-DISC1 NNNCCACTCATCCTTCAAGA NGT 2 0.0141 Tier III
38 NC_000001.11 1 38693989 + LOC105378660 NNNCCACCCATTCTTCAAGA NGC 2 0.012 Tier III
39 NC_000022.11 22 35127745 + LINC01399 NNNCCACCCATCCTTCAAGA NTC 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 25874288 + Anxa11 NNNCCTCCTATCCTTCAAGA NGG 2 0.625 Tier I
2 NC_000067.6 1 89144894 + Sh3bp4 NNNCCATCCATCCTTCAAGA NAG 2 0.2106 Tier I
3 NC_000079.6 13 36246417 + Fars2 NNNCCACCCTTCCTTCGAGA NGG 2 0.1557 Tier I
4 NC_000085.6 19 6837480 - Rps6ka4 NNNCCACCCATCCTCCCAGA NGG 2 0.0481 Tier I
5 NC_000067.6 1 162708293 + Prrc2c NNNCCAACCAACCTTCAAGA NGG 2 0.5625 Tier II
6 NC_000074.6 8 121787622 + Jph3 NNNCCACCCACACTTCAAGA NGG 2 0.4762 Tier II
7 NC_000081.6 15 82959781 + Tcf20 NNNCCGCCCATCCTACAAGA NGG 2 0.4135 Tier II
8 NC_000074.6 8 116916836 + Cmc2 NNNCCCCTCATCCTTCAAGA NGG 2 0.3977 Tier II
9 NC_000073.6 7 81229746 + Pde8a NNNCCACCCTTCCTTCAACA NGG 2 0.3955 Tier II
10 NC_000086.7 X 162533095 + Reps2 NNNCCACCCATCCTACAATA NGG 2 0.386 Tier II
11 NC_000069.6 3 88547295 + Rab25 NNNCCACCTAGCCTTCAAGA NGG 2 0.35 Tier II
12 NC_000085.6 19 41191309 - Tll2 NNNCCATCCATCCCTCAAGA NGG 2 0.2321 Tier II
13 NC_000074.6 8 114229062 + Vat1l NNNCAACCCATCCTTCAAGA NAG 2 0.1481 Tier II
14 NC_000075.6 9 69157887 + Rora NNNCCACCCATCCTTGAAGT NGG 2 0.0923 Tier II
15 NC_000071.6 5 134116591 - Castor2 NNNCCACCCATCCATCTAGA NGG 2 0.0825 Tier II
16 NC_000070.6 4 111501426 + Agbl4 NNNCCACCCATCCTTCTGGA NGG 2 0.0533 Tier II
17 NC_000076.6 10 24232303 + Moxd1 NNNTCACCCATCCTGCAAGA NGG 2 0.04 Tier II
18 NC_000074.6 8 104104111 - Cdh5 NNNCCACCCTTCCTTCAAGA NGT 2 0.0142 Tier II
19 NC_000079.6 13 14631231 + AW209491 NNNCCACCCCTCCTTCAAGA NTG 2 0.013 Tier II
20 NC_000077.6 11 94671936 + Xylt2 NNNCCACCCATCCTTCAAGA NCA 2 0.0 Tier II
21 NC_000068.7 2 25141634 + Gm13387 NNNCCACCCATCCTCCAGGA NGG 2 0.1091 Tier III
22 NC_000074.6 8 104104111 - Gm29682 NNNCCACCCTTCCTTCAAGA NGT 2 0.0142 Tier III
23 NC_000073.6 7 129658601 - Gm39094 NNNCCACCCATCCTCCAAGA NGC 2 0.0061 Tier III
24 NC_000067.6 1 185100591 + Gm34797 NNNCCACCCATCCTTCTAGA NGC 2 0.003 Tier III
25 NC_000072.6 6 25933723 + Gm20756 NNNCCACCCATCCTTCAAGA NTT 2 0.0 Tier III
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Other clones with same target sequence:

(none)