Construct: sgRNA BRDN0001147101
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGTGAGTGGCTACGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB3 (2049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75947
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
184572526 |
+ |
EPHB3 |
NNNGGTGAGTGGCTACGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
22784414 |
+ |
EPHB2 |
NNNGGTGAGTGGCTACGATG |
NGA |
1 |
0.0694 |
Tier I |
| 3 |
NC_000002.12 |
2 |
109305721 |
+ |
RANBP2 |
NNNGGTGTGTGGCTACAATG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000002.12 |
2 |
109305721 |
+ |
SH3RF3 |
NNNGGTGTGTGGCTACAATG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000019.10 |
19 |
8897484 |
- |
MUC16 |
NNNGGTGAGTGGCTGTGATG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000019.10 |
19 |
8914137 |
- |
MUC16 |
NNNGGTGAGTGGCTGTGATG |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000017.11 |
17 |
11667550 |
+ |
DNAH9 |
NNNGGTGAGTGGCTAAGCTG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000015.10 |
15 |
88906517 |
- |
MFGE8 |
NNNGGTGATTGGCTATGATG |
NGG |
2 |
0.1641 |
Tier II |
| 9 |
NC_000012.12 |
12 |
6085951 |
- |
VWF |
NNNGGTCAGTGGCTACCATG |
NGG |
2 |
0.1618 |
Tier II |
| 10 |
NC_000012.12 |
12 |
48818988 |
+ |
CACNB3 |
NNNGGTGAGTGCCCACGATG |
NGG |
2 |
0.1513 |
Tier II |
| 11 |
NC_000023.11 |
X |
135424264 |
- |
SMIM10L2A |
NNNGGTGAGAGGCTAGGATG |
NGG |
2 |
0.1319 |
Tier II |
| 12 |
NC_000016.10 |
16 |
82833827 |
+ |
CDH13 |
NNNGGTGTGTGGCTACGCTG |
NGG |
2 |
0.0816 |
Tier II |
| 13 |
NC_000019.10 |
19 |
6415367 |
- |
KHSRP |
NNNGGTGAGTGGCCACCATG |
NGG |
2 |
0.0672 |
Tier II |
| 14 |
NC_000007.14 |
7 |
42182307 |
+ |
GLI3 |
NNNGGTGAGTGGCTACCATG |
NTG |
2 |
0.0092 |
Tier II |
| 15 |
NC_000007.14 |
7 |
110784876 |
- |
IMMP2L |
NNNGGTGAGTGGCTACCATG |
NTG |
2 |
0.0092 |
Tier II |
| 16 |
NC_000002.12 |
2 |
208579553 |
- |
LOC101927960 |
NNNGGTGAGAGGCTATGATG |
NGG |
2 |
0.2637 |
Tier III |
| 17 |
NC_000002.12 |
2 |
217621913 |
+ |
DIRC3 |
NNNGGTGAGTTGCTATGATG |
NGG |
2 |
0.1183 |
Tier III |
| 18 |
NC_000005.10 |
5 |
1793649 |
+ |
LOC101929003 |
NNNGGTGAGTGGCTGCGATG |
NGA |
2 |
0.0451 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
139751799 |
- |
Pax7 |
NNNGGTGAGTGGCTAAGAAG |
NGG |
2 |
0.2857 |
Tier II |
| 2 |
NC_000072.6 |
6 |
86777516 |
- |
Anxa4 |
NNNTCTGAGTGGCTACGATG |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000083.6 |
17 |
62603221 |
+ |
Efna5 |
NNNGGTGAGTGGCTTCAATG |
NGG |
2 |
0.1867 |
Tier II |
| 4 |
NC_000070.6 |
4 |
84410214 |
- |
Bnc2 |
NNNGGTGGGTGGCTAGGATG |
NGG |
2 |
0.1128 |
Tier II |
| 5 |
NC_000070.6 |
4 |
104788093 |
- |
C8b |
NNNGGTGAGTGCCTACGATG |
NGC |
2 |
0.0118 |
Tier II |
| 6 |
NC_000075.6 |
9 |
41917299 |
- |
Gm36227 |
NNNGGACAGTGGCTACGATG |
NGG |
2 |
0.5958 |
Tier III |
| 7 |
NC_000084.6 |
18 |
5514557 |
- |
Gm10125 |
NNNGGTGAGTGGGTACCATG |
NGG |
2 |
0.0321 |
Tier III |
Other clones with same target sequence:
(none)
