Construct: sgRNA BRDN0001147105
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTGACCCCGGATGACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK6 (2870)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75705
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
177432260 |
+ |
GRK6 |
NNNTGACCCCGGATGACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
64691384 |
- |
CACHD1 |
NNNCGACCCCGGCTGACAAG |
NGG |
2 |
0.1362 |
Tier I |
| 3 |
NC_000017.11 |
17 |
61474034 |
- |
TBX4 |
NNNAGACCCCGGATGATAAG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000005.10 |
5 |
79612438 |
- |
TENT2 |
NNNTGACCCCGGATGAGAAT |
NGG |
2 |
0.0412 |
Tier II |
| 5 |
NC_000005.10 |
5 |
1120381 |
+ |
SLC12A7 |
NNNTGAACCCGGAGGACAAG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000022.11 |
22 |
20639178 |
+ |
LOC100421121 |
NNNTGACCAGGGATGACAAG |
NGG |
2 |
0.3333 |
Tier III |
| 7 |
NC_000008.11 |
8 |
102529542 |
+ |
LINC03047 |
NNNTGACCTCGGATGACACG |
NGG |
2 |
0.181 |
Tier III |
| 8 |
NC_000013.11 |
13 |
21319444 |
+ |
GRK6P1 |
NNNCGACCCCGGATGAGAAG |
NGG |
2 |
0.0381 |
Tier III |
| 9 |
NC_000013.11 |
13 |
21319444 |
+ |
MIPEPP3 |
NNNCGACCCCGGATGAGAAG |
NGG |
2 |
0.0381 |
Tier III |
| 10 |
NC_000013.11 |
13 |
21319444 |
+ |
LINC00539 |
NNNCGACCCCGGATGAGAAG |
NGG |
2 |
0.0381 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
55450534 |
+ |
Grk6 |
NNNTGACCCCTGATGAGAAG |
NGG |
2 |
0.0226 |
Tier I |
| 2 |
NC_000068.7 |
2 |
120061808 |
+ |
Sptbn5 |
NNNTGACCCCAGAAGACAAG |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000074.6 |
8 |
119719552 |
- |
Atp2c2 |
NNNTGAGCCCCGATGACAAG |
NGG |
2 |
0.2017 |
Tier II |
| 4 |
NC_000081.6 |
15 |
84951836 |
+ |
5031439G07Rik |
NNNTGACCCCGGAGGACAAG |
NAG |
2 |
0.0 |
Tier II |
| 5 |
NC_000068.7 |
2 |
120061808 |
+ |
Gm52559 |
NNNTGACCCCAGAAGACAAG |
NGG |
2 |
0.619 |
Tier III |
| 6 |
NC_000074.6 |
8 |
90759656 |
- |
Gm35963 |
NNNTTACCACGGATGACAAG |
NGG |
2 |
0.2571 |
Tier III |
| 7 |
NC_000067.6 |
1 |
73192954 |
- |
Gm29185 |
NNNTGAGCCCGGATGACAAC |
NGG |
2 |
0.2017 |
Tier III |
| 8 |
NC_000068.7 |
2 |
133427928 |
+ |
Gm31851 |
NNNTGACCCCAGATTACAAG |
NGG |
2 |
0.1429 |
Tier III |
Other clones with same target sequence:
(none)
