Construct: sgRNA BRDN0001147108
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGTAGTTTCCTTCCAACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK24 (8428)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77055
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
98466445 |
+ |
STK24 |
NNNTAGTTTCCTTCCAACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
21671758 |
- |
KCNIP4 |
NNNTAGTTTTCCTCCAACGT |
NGG |
2 |
0.8916 |
Tier II |
| 3 |
NC_000006.12 |
6 |
20117597 |
+ |
MBOAT1 |
NNNTAGTTTCCACCCAACGT |
NGG |
2 |
0.6316 |
Tier II |
| 4 |
NC_000023.11 |
X |
124499239 |
+ |
TENM1 |
NNNTAGTTTTCTTCCAACTT |
NGG |
2 |
0.6275 |
Tier II |
| 5 |
NC_000008.11 |
8 |
50069261 |
+ |
SNTG1 |
NNNTAGTTTCCTCCCAACTT |
NGG |
2 |
0.5263 |
Tier II |
| 6 |
NC_000022.11 |
22 |
33079710 |
+ |
LARGE1 |
NNNTAGTTTCCTTACAAAGT |
NGG |
2 |
0.1885 |
Tier II |
| 7 |
NC_000009.12 |
9 |
122899630 |
+ |
RC3H2 |
NNNTAGTTTCCTTCCAACTT |
NAG |
2 |
0.1728 |
Tier II |
| 8 |
NC_000010.11 |
10 |
75672893 |
+ |
LRMDA |
NNNTTGTTTCCTTCCACCGT |
NGG |
2 |
0.0642 |
Tier II |
| 9 |
NC_000014.9 |
14 |
52439705 |
+ |
TXNDC16 |
NNNTAGTTTCCTTCCAACAT |
NTG |
2 |
0.0278 |
Tier II |
| 10 |
NC_000009.12 |
9 |
130623568 |
+ |
FUBP3 |
NNNTTGTTTCCTTCTAACGT |
NGG |
2 |
0.0242 |
Tier II |
| 11 |
NC_000005.10 |
5 |
171294629 |
- |
RANBP17 |
NNNTAGTTTCCTTCCAAGGC |
NGG |
2 |
0.0121 |
Tier II |
| 12 |
NC_000004.12 |
4 |
75966547 |
+ |
SDAD1 |
NNNTAGTTTCCTTCCTACTT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000023.11 |
X |
136296265 |
+ |
STK24P1 |
NNNTAGTTTTCTTCCAACGT |
NGG |
1 |
0.9412 |
Tier III |
| 14 |
NC_000004.12 |
4 |
189834182 |
- |
FRG1-DT |
NNNTAATTTCCTTTCAACGT |
NGG |
2 |
0.7333 |
Tier III |
| 15 |
NC_000003.12 |
3 |
108721697 |
+ |
LOC105374033 |
NNNGAGTTTCCTTCCAAAGT |
NGG |
2 |
0.3365 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
33568143 |
+ |
Exoc4 |
NNNTAATTTCCTTCCAACTT |
NGG |
2 |
0.6667 |
Tier II |
| 2 |
NC_000070.6 |
4 |
62658785 |
+ |
Rgs3 |
NNNTATTTTCCTTCCAACTT |
NGG |
2 |
0.4444 |
Tier II |
| 3 |
NC_000085.6 |
19 |
42920289 |
- |
Hpse2 |
NNNTAGTTTCCTTCAAACGT |
NGG |
1 |
0.2222 |
Tier II |
| 4 |
NC_000071.6 |
5 |
115845260 |
+ |
Cit |
NNNTCGTTTCCTTCAAACGT |
NGG |
2 |
0.1111 |
Tier II |
| 5 |
NC_000079.6 |
13 |
113829147 |
- |
Arl15 |
NNNTAGTTTCCTTCCAACAT |
NGA |
2 |
0.0496 |
Tier II |
| 6 |
NC_000068.7 |
2 |
173660389 |
+ |
Rab22a |
NNNTAGTTTCCTTCCAACTT |
NGC |
2 |
0.0148 |
Tier II |
| 7 |
NC_000077.6 |
11 |
33218640 |
+ |
Ranbp17 |
NNNTAGTTTCCTTCCAAGGC |
NGG |
2 |
0.0121 |
Tier II |
| 8 |
NC_000080.6 |
14 |
104696213 |
- |
D130009I18Rik |
NNNTTCTTTCCTTCCAACGT |
NGG |
2 |
0.2479 |
Tier III |
| 9 |
NC_000078.6 |
12 |
77751114 |
+ |
Gm35440 |
NNNTAGTCTCCTTCCAACGC |
NGG |
2 |
0.0909 |
Tier III |
Other clones with same target sequence:
(none)
