Construct: sgRNA BRDN0001147116
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCCGTGTCTAGCCCAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRB (5159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77060
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150135647 |
+ |
PDGFRB |
NNNCCGTGTCTAGCCCAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
46190592 |
- |
LSS |
NNNCCGTGAATAGCCCAGTG |
NGG |
2 |
0.8048 |
Tier II |
| 3 |
NC_000011.10 |
11 |
44276098 |
+ |
ALX4 |
NNNCCATGTCTGGCCCAGTG |
NGG |
2 |
0.7222 |
Tier II |
| 4 |
NC_000017.11 |
17 |
76887859 |
+ |
MGAT5B |
NNNCCGTGTCTGGCCCAGTG |
NGG |
1 |
0.7222 |
Tier II |
| 5 |
NC_000003.12 |
3 |
4152595 |
+ |
SUMF1 |
NNNCCGTGCCTGGCCCAGTG |
NGG |
2 |
0.6667 |
Tier II |
| 6 |
NC_000021.9 |
21 |
32426130 |
+ |
EVA1C |
NNNCCCTCTCTAGCCCAGTG |
NGG |
2 |
0.4196 |
Tier II |
| 7 |
NC_000014.9 |
14 |
55344980 |
+ |
FBXO34 |
NNNCAGTGTCTAGCCCAGAG |
NGG |
2 |
0.1633 |
Tier II |
| 8 |
NC_000014.9 |
14 |
55345685 |
+ |
FBXO34 |
NNNCAGTGTCTAGCCCAGAG |
NGG |
2 |
0.1633 |
Tier II |
| 9 |
NC_000016.10 |
16 |
1451970 |
+ |
CLCN7 |
NNNCCGTGTCTAGCCCTGGG |
NGG |
2 |
0.0333 |
Tier II |
| 10 |
NC_000011.10 |
11 |
3952060 |
- |
STIM1 |
NNNCTGTGTCTAGCCCAGTG |
NTG |
2 |
0.0248 |
Tier II |
| 11 |
NC_000016.10 |
16 |
65347686 |
- |
LINC00922 |
NNNCAGTGTCAAGCCCAGTG |
NGG |
2 |
0.4286 |
Tier III |
| 12 |
NC_000016.10 |
16 |
65347686 |
- |
LOC124903780 |
NNNCAGTGTCAAGCCCAGTG |
NGG |
2 |
0.4286 |
Tier III |
| 13 |
NC_000002.12 |
2 |
98768695 |
+ |
LINC02611 |
NNNCTGTGTCTACCCCAGTG |
NGG |
2 |
0.2679 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
35559209 |
+ |
Slit3 |
NNNCCTTATCTAGCCCAGTG |
NGG |
2 |
0.6667 |
Tier II |
| 2 |
NC_000070.6 |
4 |
104701994 |
- |
Dab1 |
NNNTGGTGTCTAGCCCAGTG |
NGG |
2 |
0.48 |
Tier II |
| 3 |
NC_000074.6 |
8 |
106014870 |
- |
Dus2 |
NNNCAGTGTCCAGCCCAGTG |
NGG |
2 |
0.381 |
Tier II |
| 4 |
NC_000077.6 |
11 |
5945785 |
- |
Gck |
NNNCCGTGTGTGGCCCAGTG |
NGG |
2 |
0.2809 |
Tier II |
| 5 |
NC_000079.6 |
13 |
105102062 |
- |
Nt5el |
NNNCAGTGTCTAGCCCACTG |
NGG |
2 |
0.2721 |
Tier II |
| 6 |
NC_000076.6 |
10 |
71124741 |
- |
Bicc1 |
NNNCCGTGTCTGGACCAGTG |
NGG |
2 |
0.2528 |
Tier II |
| 7 |
NC_000072.6 |
6 |
32357661 |
+ |
Plxna4 |
NNNCCTTGTCTAGCCCAGAG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000073.6 |
7 |
141985521 |
- |
Brsk2 |
NNNCCGTGTCTAGCCCAGTG |
NTA |
2 |
0.0 |
Tier II |
| 9 |
NC_000084.6 |
18 |
4886024 |
+ |
Gm41659 |
NNNCCGTGTCTAATCCAGTG |
NGG |
2 |
0.6769 |
Tier III |
Other clones with same target sequence:
(none)
