Construct: sgRNA BRDN0001147117
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAAGTTACTACATTGCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME5 (8382)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76572
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
138129443 |
+ |
NME5 |
NNNAGTTACTACATTGCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
130156126 |
- |
CHSY3 |
NNNAGTAACTAAATTGCTCA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000002.12 |
2 |
149105442 |
- |
LYPD6B |
NNNAGTTACTGCATTGTTCA |
NGG |
2 |
0.3033 |
Tier II |
| 4 |
NC_000006.12 |
6 |
75150677 |
- |
COL12A1 |
NNNAGTTACTACATTGCTAT |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000003.12 |
3 |
151753689 |
- |
AADACL2 |
NNNAGTTCCTACATTGCTAA |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000003.12 |
3 |
114407302 |
+ |
ZBTB20 |
NNNAGTTACTTCTTTGCTCA |
NGG |
2 |
0.0923 |
Tier II |
| 7 |
NC_000011.10 |
11 |
103331228 |
+ |
DYNC2H1 |
NNNAGTTAATACATTGGTCA |
NGG |
2 |
0.0504 |
Tier II |
| 8 |
NC_000020.11 |
20 |
37984962 |
+ |
TTI1 |
NNNAGTTACTACATTGCACA |
NGA |
2 |
0.0463 |
Tier II |
| 9 |
NC_000014.9 |
14 |
37674936 |
- |
TTC6 |
NNNAGTTACTACATTGCCCA |
NTG |
2 |
0.0167 |
Tier II |
| 10 |
NC_000004.12 |
4 |
79315142 |
- |
NAA11 |
NNNAGTTCCTACATTGCTCA |
NGT |
2 |
0.0069 |
Tier II |
| 11 |
NC_000003.12 |
3 |
29645110 |
+ |
RBMS3 |
NNNAGTTCCTACATTTCTCA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000015.10 |
15 |
43573675 |
- |
PPIP5K1 |
NNNAGTTGCTACATTTCTCA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000013.11 |
13 |
55069128 |
+ |
LINC02335 |
NNNAGTCACTACATTACTCA |
NGG |
2 |
0.6875 |
Tier III |
| 14 |
NC_000003.12 |
3 |
151753689 |
- |
AADACL2-AS1 |
NNNAGTTCCTACATTGCTAA |
NGG |
2 |
0.1837 |
Tier III |
| 15 |
NC_000020.11 |
20 |
41693169 |
- |
LOC124904905 |
NNNCGTTACTACATTGCCCA |
NGG |
2 |
0.1513 |
Tier III |
| 16 |
NC_000015.10 |
15 |
43673240 |
- |
PPIP5K1P1 |
NNNAGTTGCTACATTTCTCA |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000015.10 |
15 |
43673240 |
- |
PPIP5K1P1-CATSPER2 |
NNNAGTTGCTACATTTCTCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
34571636 |
+ |
Nme5 |
NNNAGTTACTACAGTGCTCG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
10700431 |
+ |
Clec16a |
NNNTGTTGCTACATTGCTCA |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000077.6 |
11 |
55030993 |
+ |
Anxa6 |
NNNACTTACTACATAGCTCA |
NGG |
2 |
0.4549 |
Tier II |
| 4 |
NC_000081.6 |
15 |
20604213 |
+ |
Cdh12 |
NNNACTTACTACATAGCTCA |
NGG |
2 |
0.4549 |
Tier II |
| 5 |
NC_000086.7 |
X |
144717209 |
- |
Rtl4 |
NNNATTTACTACATTGCACA |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000083.6 |
17 |
25927445 |
- |
Pigq |
NNNAGTTACTACTTTGCTCT |
NGG |
2 |
0.18 |
Tier II |
| 7 |
NC_000073.6 |
7 |
138880958 |
- |
Ppp2r2d |
NNNAGGTACTACTTTGCTCA |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000083.6 |
17 |
28608326 |
+ |
Srpk1 |
NNNAGTTACTACATAGCTCA |
NAG |
2 |
0.1501 |
Tier II |
| 9 |
NC_000067.6 |
1 |
160271012 |
+ |
Rabgap1l |
NNNAGTTAGTACATTGCTGA |
NGG |
2 |
0.0774 |
Tier II |
| 10 |
NC_000068.7 |
2 |
181311143 |
+ |
Stmn3 |
NNNAGTTACTCCATTCCTCA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000077.6 |
11 |
36783316 |
+ |
Tenm2 |
NNNAGTTATTACATTCCTCA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000075.6 |
9 |
47768032 |
- |
Cadm1 |
NNNAGTTATTACATTTCTCA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000073.6 |
7 |
117313443 |
- |
Gm39075 |
NNNAGTTACTTCTTTGCTCA |
NGG |
2 |
0.0923 |
Tier III |
Other clones with same target sequence:
(none)
