Construct: sgRNA BRDN0001147123
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCACCAGAGATTGTGCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK16 (5127)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76902
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
47224394 |
+ |
CDK16 |
NNNACCAGAGATTGTGCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
159816010 |
+ |
PNLDC1 |
NNNACCAGAGATTGTTCACG |
NGA |
2 |
0.0 |
Tier I |
| 3 |
NC_000011.10 |
11 |
100142880 |
+ |
CNTN5 |
NNNACCACAGATTGTCCACG |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000012.12 |
12 |
69517185 |
+ |
FRS2 |
NNNACCACAGATTGTCCACG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000016.10 |
16 |
12773794 |
- |
CPPED1 |
NNNACCACAGATTGTTCACG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000007.14 |
7 |
18588596 |
- |
HDAC9 |
NNNACCAGAGATTGTCCACA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000014.9 |
14 |
32076434 |
- |
ARHGAP5-AS1 |
NNNACCAGATATTGTGCTCG |
NGG |
2 |
0.4063 |
Tier III |
| 8 |
NC_000020.11 |
20 |
50351254 |
+ |
LOC105372657 |
NNNACCACAGATTGTCCACG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
20694047 |
+ |
Cdk16 |
NNNCCCAGAGATTGTGCACG |
NGG |
1 |
0.3529 |
Tier I |
| 2 |
NC_000067.6 |
1 |
167056762 |
+ |
Fam78b |
NNNACCAGTGATTGTGCACA |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000084.6 |
18 |
23814825 |
- |
Mapre2 |
NNNACCAGGGATTCTGCACG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000080.6 |
14 |
25884118 |
- |
Anxa11 |
NNNACCAGAGTATGTGCACG |
NGG |
2 |
0.2462 |
Tier II |
| 5 |
NC_000069.6 |
3 |
55987518 |
+ |
Nbea |
NNNACCTGAGAGTGTGCACG |
NGG |
2 |
0.2188 |
Tier II |
| 6 |
NC_000068.7 |
2 |
66194033 |
- |
Ttc21b |
NNNACCAGAGATTGTGCCAG |
NGG |
2 |
0.0816 |
Tier II |
| 7 |
NC_000083.6 |
17 |
26642955 |
+ |
Ergic1 |
NNNACCAGAGATTGTGAAGG |
NGG |
2 |
0.0583 |
Tier II |
| 8 |
NC_000067.6 |
1 |
16598929 |
- |
Ube2w |
NNNTCCAGAGATTGTGGACG |
NGG |
2 |
0.0374 |
Tier II |
| 9 |
NC_000071.6 |
5 |
31620860 |
+ |
Mrpl33 |
NNNACCAGAGATTCTGGACG |
NGG |
2 |
0.0252 |
Tier II |
| 10 |
NC_000073.6 |
7 |
139497213 |
+ |
Inpp5a |
NNNACCAGAGATTGTGGAGG |
NGG |
2 |
0.0074 |
Tier II |
| 11 |
NC_000080.6 |
14 |
26023888 |
- |
Gm2260 |
NNNACCAGAGTATGTGCACG |
NGG |
2 |
0.2462 |
Tier III |
| 12 |
NC_000080.6 |
14 |
26163502 |
- |
Gm2274 |
NNNACCAGAGTATGTGCACG |
NGG |
2 |
0.2462 |
Tier III |
| 13 |
NC_000069.6 |
3 |
55987518 |
+ |
Gm31914 |
NNNACCTGAGAGTGTGCACG |
NGG |
2 |
0.2188 |
Tier III |
| 14 |
NC_000083.6 |
17 |
26642955 |
+ |
Gm41553 |
NNNACCAGAGATTGTGAAGG |
NGG |
2 |
0.0583 |
Tier III |
| 15 |
NC_000071.6 |
5 |
31620860 |
+ |
Gm38424 |
NNNACCAGAGATTCTGGACG |
NGG |
2 |
0.0252 |
Tier III |
Other clones with same target sequence:
(none)
