Construct: sgRNA BRDN0001147125

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGACTGGAACATGAAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK16 (5127)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76903

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 47225072 + CDK16 NNNGACTGGAACATGAAGAG NGG 0 1.0 Tier I
2 NC_000022.11 22 45372538 + SMC1B NNNGAATGGAAAATGAAGAG NGG 2 0.6633 Tier II
3 NC_000002.12 2 134178785 - MGAT5 NNNGACAGAAACATGAAGAG NGG 2 0.5625 Tier II
4 NC_000023.11 X 124526833 - TENM1 NNNGACTAGAAGATGAAGAG NGG 2 0.4444 Tier II
5 NC_000010.11 10 99220842 + HPSE2 NNNGAATGGAACATGAAGGG NGG 2 0.3482 Tier II
6 NC_000013.11 13 57669384 - PCDH17 NNNGAGGGGAACATGAAGAG NGG 2 0.2941 Tier II
7 NC_000014.9 14 39353329 - MIA2 NNNGAGGGGAACATGAAGAG NGG 2 0.2941 Tier II
8 NC_000012.12 12 39406019 - KIF21A NNNGACTTGAACATGAAGGG NGG 2 0.2344 Tier II
9 NC_000001.11 1 222831685 + DISP1 NNNGACTGGAACTTGAAGAT NGG 2 0.21 Tier II
10 NC_000011.10 11 63357455 - SLC22A10 NNNGACTGAATCATGAAGAG NGG 2 0.1978 Tier II
11 NC_000007.14 7 49947601 + ZPBP NNNGACTGGAACAAGAGGAG NGG 2 0.1092 Tier II
12 NC_000002.12 2 6849649 + CMPK2 NNNCACTGGAACATGATGAG NGG 2 0.0706 Tier II
13 NC_000019.10 19 33204506 + LRP3 NNNGTCTGGAACATGAGGAG NGG 2 0.0642 Tier II
14 NC_000012.12 12 128282548 - TMEM132C NNNGACTGGATCATGAGGAG NGG 2 0.0543 Tier II
15 NC_000015.10 15 84033748 - ADAMTSL3 NNNGGCTGGAACATGAAGAG NGA 2 0.05 Tier II
16 NC_000005.10 5 151561294 + FAT2 NNNGACTGGAACATGGGGAG NGG 2 0.0339 Tier II
17 NC_000005.10 5 156925404 - TIMD4 NNNGAATGGAACAGGAAGAG NGG 2 0.0 Tier II
18 NC_000003.12 3 68518724 + TAFA1 NNNGAATGGAACATGTAGAG NGG 2 0.0 Tier II
19 NC_000023.11 X 137033135 + GPR101 NNNGACTGGAAAATGCAGAG NGG 2 0.0 Tier II
20 NC_000013.11 13 43253257 - ENOX1 NNNGACTGGAACAGGAAGAG NTG 2 0.0 Tier II
21 NC_000001.11 1 47025811 - CYP4X1 NNNGACTGGAACAGGAGGAG NGG 2 0.0 Tier II
22 NC_000010.11 10 79214243 + ZMIZ1 NNNGACTGGGACATGTAGAG NGG 2 0.0 Tier II
23 NC_000009.12 9 123642715 + DENND1A NNNGGCTGGAACATGCAGAG NGG 2 0.0 Tier II
24 NC_000007.14 7 150664482 + LOC124901774 NNNGATTGGAACATGAAGAA NGG 2 0.8705 Tier III
25 NC_000001.11 1 119244706 - WARS2-AS1 NNNGAATGGAAAATGAAGAG NGG 2 0.6633 Tier III
26 NC_000009.12 9 11182150 - LOC102724027 NNNGAATGCAACATGAAGAG NGG 2 0.5 Tier III
27 NC_000009.12 9 1324721 - LOC102723803 NNNGACTGTAACATGAAAAG NGG 2 0.3692 Tier III
28 NC_000005.10 5 77903187 + LOC101929154 NNNAACTGGAACATGAAGAG NAG 2 0.2333 Tier III
29 NC_000001.11 1 202998398 + MGAT4FP NNNGACTGGATCATGAAGTG NGG 2 0.1657 Tier III
30 NC_000007.14 7 20134009 - LOC101927668 NNNAACTGGAACATGAGGAG NGG 2 0.1588 Tier III
31 NC_000008.11 8 126692550 - LOC105375751 NNNCACTGGAACATGAAGAG NAG 2 0.1373 Tier III
32 NC_000001.11 1 220746519 - LOC124904516 NNNGACTTGAACATGAGGAG NGG 2 0.1103 Tier III
33 NC_000009.12 9 115993076 - LOC105376236 NNNGACTGGAACAGGAAGAG NGT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 59158339 + Mia2 NNNGACTTGGACATGAAGAG NGG 2 0.3472 Tier I
2 NC_000086.7 X 20694716 + Cdk16 NNNGACTGGAACACGAAGAA NGG 2 0.2679 Tier I
3 NC_000071.6 5 128829991 + Rimbp2 NNNGACTGGAACATGAAGAG NGG 0 1.0 Tier II
4 NC_000085.6 19 41646587 - Slit1 NNNGACAGGAACATGAAGAA NGG 2 0.8203 Tier II
5 NC_000082.6 16 40509864 - Lsamp NNNAGCTGGAACATGAAGAG NGG 2 0.648 Tier II
6 NC_000074.6 8 91625601 + Fto NNNGACAGGAACATGAATAG NGG 2 0.5833 Tier II
7 NC_000077.6 11 88490656 + Msi2 NNNGACAGGAACAAGAAGAG NGG 2 0.5417 Tier II
8 NC_000073.6 7 99884137 - Xrra1 NNNGGCTGGAACAAGAAGAG NGG 2 0.4457 Tier II
9 NC_000075.6 9 117357641 + Rbms3 NNNGACTGGAAAAAGAAGAG NGG 2 0.4422 Tier II
10 NC_000072.6 6 92693744 + Prickle2 NNNGCCAGGAACATGAAGAG NGG 2 0.4375 Tier II
11 NC_000068.7 2 62794175 + Kcnh7 NNNGACTGGAGTATGAAGAG NGG 2 0.35 Tier II
12 NC_000086.7 X 69517316 + Aff2 NNNGACTTCAACATGAAGAG NGG 2 0.3365 Tier II
13 NC_000077.6 11 107392071 - Pitpnc1 NNNGACTGTAACAAGAAGAG NGG 2 0.3302 Tier II
14 NC_000073.6 7 110853332 + Lyve1 NNNCACTGCAACATGAAGAG NGG 2 0.2851 Tier II
15 NC_000077.6 11 61254135 + Aldh3a2 NNNGACTGGAAGAAGAAGAG NGG 2 0.2751 Tier II
16 NC_000074.6 8 68444972 - Csgalnact1 NNNTACTGAAACATGAAGAG NGG 2 0.2338 Tier II
17 NC_000068.7 2 132021843 - Rassf2 NNNGACTGGCATATGAAGAG NGG 2 0.1795 Tier II
18 NC_000084.6 18 37937720 - Hdac3 NNNGTCTGGAACTTGAAGAG NGG 2 0.1091 Tier II
19 NC_000074.6 8 129064410 + Ccdc7a NNNGTCTGGAACATGAAGAG NAG 2 0.0943 Tier II
20 NC_000074.6 8 129068219 - Ccdc7b NNNGTCTGGAACATGAAGAG NAG 2 0.0943 Tier II
21 NC_000071.6 5 131632533 - Auts2 NNNGACTGGAAGATGGAGAG NGG 2 0.0855 Tier II
22 NC_000081.6 15 92083659 + Cntn1 NNNGACTGGAACATGAAAAG NCG 2 0.0742 Tier II
23 NC_000067.6 1 188669552 - Ush2a NNNGACTGGAACATGATGGG NGG 2 0.05 Tier II
24 NC_000078.6 12 100903731 - Gpr68 NNNGTCTGGAACATGAAGAG NGA 2 0.0253 Tier II
25 NC_000074.6 8 127214876 - Pard3 NNNGAATGGAACATGCAGAG NGG 2 0.0 Tier II
26 NC_000075.6 9 60334892 - Thsd4 NNNGACTGGTACATGCAGAG NGG 2 0.0 Tier II
27 NC_000068.7 2 38013892 + Dennd1a NNNGGCTGGAACATGCAGAG NGG 2 0.0 Tier II
28 NC_000085.6 19 23513292 - Gm34012 NNNGGCAGGAACATGAAGAG NGG 2 0.63 Tier III
29 NC_000071.6 5 67456404 - Gm40315 NNNGACTGAAAAATGAAGAG NGG 2 0.4592 Tier III
30 NC_000073.6 7 99884137 - Gm38405 NNNGGCTGGAACAAGAAGAG NGG 2 0.4457 Tier III
31 NC_000068.7 2 85157847 - Gm13712 NNNGACTTGGACATGAAGAG NGG 2 0.3472 Tier III
32 NC_000068.7 2 43741484 - Gm39804 NNNAACTGGAACATGAAGAG NAG 2 0.2333 Tier III
33 NC_000073.6 7 117235086 + Gm39075 NNNTACTGGAACAAGAAGAG NGG 2 0.2251 Tier III
34 NC_000078.6 12 100903731 - Gm36572 NNNGTCTGGAACATGAAGAG NGA 2 0.0253 Tier III
35 NC_000067.6 1 71684869 - Gm46094 NNNGAATGGAACAGGAAGAG NGG 2 0.0 Tier III
36 NC_000067.6 1 120650123 + 2610027F03Rik NNNGACTGGAACAGGAAGAG NTG 2 0.0 Tier III
37 NC_000075.6 9 60334892 - 2010001M07Rik NNNGACTGGTACATGCAGAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)