Construct: sgRNA BRDN0001147131
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTCACCGAAGACTTCAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKD (8527)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77258
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
233446783 |
+ |
DGKD |
NNNCACCGAAGACTTCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
37207343 |
+ |
RPN2 |
NNNCTCCGAAGCCTTCAGCG |
NGG |
2 |
0.0957 |
Tier I |
| 3 |
NC_000016.10 |
16 |
82983105 |
+ |
CDH13 |
NNNCACCAAAGACTTCAGCA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000009.12 |
9 |
4429218 |
- |
GLIS3 |
NNNCACAGAAGATTTCAGCG |
NGG |
2 |
0.525 |
Tier II |
| 5 |
NC_000001.11 |
1 |
21634855 |
+ |
RAP1GAP |
NNNCACCGAGGACTTCAGTG |
NGG |
2 |
0.2564 |
Tier II |
| 6 |
NC_000019.10 |
19 |
3268826 |
+ |
CELF5 |
NNNCACGGAAGACTTCAGGG |
NGG |
2 |
0.0588 |
Tier II |
| 7 |
NC_000016.10 |
16 |
82983105 |
+ |
LOC101928417 |
NNNCACCAAAGACTTCAGCA |
NGG |
2 |
0.9375 |
Tier III |
| 8 |
NC_000008.11 |
8 |
124152078 |
+ |
FER1L6-AS2 |
NNNCACCTTAGACTTCAGCG |
NGG |
2 |
0.375 |
Tier III |
| 9 |
NC_000008.11 |
8 |
124152078 |
+ |
ARF1P3 |
NNNCACCTTAGACTTCAGCG |
NGG |
2 |
0.375 |
Tier III |
| 10 |
NC_000021.9 |
21 |
25491460 |
+ |
LOC105372753 |
NNNCACCGCAGACTTCAGCG |
NGA |
2 |
0.0397 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
78918235 |
+ |
Pdxp |
NNNCACAGAAGACTTCAGCG |
NGG |
1 |
0.75 |
Tier I |
| 2 |
NC_000067.6 |
1 |
87924551 |
+ |
Dgkd |
NNNCACTGAGGACTTCAGCG |
NGG |
2 |
0.4514 |
Tier I |
| 3 |
NC_000074.6 |
8 |
3380304 |
+ |
Arhgef18 |
NNNCACAGAAGACTTCAGCC |
NGG |
2 |
0.3214 |
Tier I |
| 4 |
NC_000077.6 |
11 |
109249004 |
+ |
Rgs9 |
NNNCACCGAAGACTTCACTG |
NGG |
2 |
0.2198 |
Tier I |
| 5 |
NC_000068.7 |
2 |
156704590 |
+ |
Dlgap4 |
NNNCACCCAAGACTGCAGCG |
NGG |
2 |
0.0308 |
Tier I |
| 6 |
NC_000076.6 |
10 |
127287962 |
+ |
Mbd6 |
NNNCACAGAAGACTTCAGCT |
NGG |
2 |
0.525 |
Tier II |
| 7 |
NC_000067.6 |
1 |
59214360 |
- |
Als2 |
NNNCACTGAAGACTTCAGAG |
NGG |
2 |
0.3482 |
Tier II |
| 8 |
NC_000073.6 |
7 |
140047384 |
- |
Msx3 |
NNNCACCGAGGACTTCAGAG |
NGG |
2 |
0.2381 |
Tier II |
| 9 |
NC_000081.6 |
15 |
78918235 |
+ |
Gm30368 |
NNNCACAGAAGACTTCAGCG |
NGG |
1 |
0.75 |
Tier III |
| 10 |
NC_000068.7 |
2 |
156704590 |
+ |
Gm34111 |
NNNCACCCAAGACTGCAGCG |
NGG |
2 |
0.0308 |
Tier III |
Other clones with same target sequence:
(none)
