Construct: sgRNA BRDN0001147140

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAGGTCCTGTCAACAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA3 (2042)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76745

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 89210234 + EPHA3 NNNGGTCCTGTCAACAAGAA NGG 0 1.0 Tier I
2 NC_000005.10 5 141578354 + DIAPH1 NNNGGTCCTGTCAACAACAA NAG 2 0.1235 Tier I
3 NC_000013.11 13 75416234 + TBC1D4 NNNAGTCATGTCAACAAGAA NGG 2 0.585 Tier II
4 NC_000001.11 1 43865745 - ST3GAL3 NNNGGTCCTGTAAACAATAA NGG 2 0.4762 Tier II
5 NC_000007.14 7 83590446 - SEMA3E NNNGGTTCTGTCATCAAGAA NGG 2 0.4333 Tier II
6 NC_000008.11 8 26648408 - DPYSL2 NNNGGTCCTGTTAACAAGAG NGG 2 0.4118 Tier II
7 NC_000016.10 16 25936141 + HS3ST4 NNNGGTCCTGTTGACAAGAA NGG 2 0.3512 Tier II
8 NC_000021.9 21 45857777 - PCBP3 NNNGCTCCTGTCAACAAGAA NAG 2 0.2037 Tier II
9 NC_000005.10 5 40969647 - C7 NNNAGTCCTGTCAAAAAGAA NGG 2 0.2 Tier II
10 NC_000001.11 1 20602124 + CDA NNNGATCCTGTCAAAAAGAA NGG 2 0.1926 Tier II
11 NC_000002.12 2 80088397 - CTNNA2 NNNGGTCATGTCAACAGGAA NGG 2 0.1147 Tier II
12 NC_000023.11 X 129646615 + APLN NNNGGTCCGGTCAACACGAA NGG 2 0.1092 Tier II
13 NC_000020.11 20 2956495 - PTPRA NNNGGTCCTGGCAACAAGAA NGA 2 0.0278 Tier II
14 NC_000012.12 12 132886484 - CHFR NNNGGTCCTGTCAACAAAAA NGT 2 0.0112 Tier II
15 NC_000012.12 12 53058125 + TNS2 NNNGGTCCTGTCAACAAGAA NAA 2 0.0 Tier II
16 NC_000005.10 5 38790012 + OSMR-DT NNNGGTTCTGTTAACAAGAA NGG 2 0.4375 Tier III
17 NC_000005.10 5 38790012 + LOC107986414 NNNGGTTCTGTTAACAAGAA NGG 2 0.4375 Tier III
18 NC_000007.14 7 101583647 + LOC107986832 NNNGGTCCTTTCAACAACAA NGG 2 0.3869 Tier III
19 NC_000002.12 2 110680009 - LOC105373554 NNNGGTCCTGGCATCAAGAA NGG 2 0.2133 Tier III
20 NC_000005.10 5 176174557 - CEP192P1 NNNGGTCCTGGCATCAAGAA NGG 2 0.2133 Tier III
21 NC_000003.12 3 144339146 + LOC105374140 NNNGGTCCTGTGAACAAGAA NAG 2 0.1152 Tier III
22 NC_000003.12 3 150018926 + LOC124909445 NNNGGTCCTGTCAAAAGGAA NGG 2 0.0392 Tier III
23 NC_000003.12 3 131504540 + LOC105374114 NNNGGTGCTGTCAAGAAGAA NGG 2 0.0235 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 97961470 - Plxdc1 NNNGGACCTGCCAACAAGAA NGG 2 0.5778 Tier II
2 NC_000074.6 8 75565984 + Iqcm NNNAGTCCTGTCAACAAGGA NGG 2 0.3375 Tier II
3 NC_000070.6 4 14766447 - Lrrc69 NNNGGTGCTGTCATCAAGAA NGG 2 0.251 Tier II
4 NC_000081.6 15 81857557 + Tob2 NNNGGCCCTGTCACCAAGAA NGG 2 0.1948 Tier II
5 NC_000070.6 4 95634607 + Fggy NNNTGTCCTGTCTACAAGAA NGG 2 0.1091 Tier II
6 NC_000078.6 12 78654443 + Gphn NNNGGTCCTGTCAACACGTA NGG 2 0.095 Tier II
7 NC_000083.6 17 86799646 - Epas1 NNNGGTCCTGTCTAAAAGAA NGG 2 0.0667 Tier II
8 NC_000070.6 4 77309484 + Ptprd NNNGTTCCTGTCAACGAGAA NGG 2 0.0577 Tier II
9 NC_000085.6 19 6229892 + Ppp2r5b NNNGTTCCTGTCAACATGAA NGG 2 0.04 Tier II
10 NC_000067.6 1 190797118 + Rps6kc1 NNNGGTCCTGTTAACAAGAA NGA 2 0.0374 Tier II
11 NC_000067.6 1 151612336 + Niban1 NNNGGTCCTTTCAACAAGAA NTG 2 0.0317 Tier II
12 NC_000075.6 9 117557573 + Rbms3 NNNGGTCCTGTGAAGAAGAA NGG 2 0.0222 Tier II
13 NC_000067.6 1 51812040 + Myo1b NNNTGTCCTGTCAACAAGAA NTG 2 0.0142 Tier II
14 NC_000079.6 13 65343393 + Gm7762 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
15 NC_000079.6 13 65409688 + Gm31714 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
16 NC_000079.6 13 65552259 + Gm19428 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
17 NC_000079.6 13 65695306 + Gm53057 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
18 NC_000079.6 13 65954606 - Gm46425 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
19 NC_000079.6 13 66117333 - Gm10324 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
20 NC_000079.6 13 66436091 + 2410141K09Rik NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
21 NC_000079.6 13 66675624 + Zfp640 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
22 NC_000079.6 13 66857165 + Gm10323 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier II
23 NC_000067.6 1 102152824 - Gm3116 NNNGGTCCTGTAAACAAGAA NGG 1 0.7143 Tier III
24 NC_000076.6 10 112011101 + Gm7269 NNNGGGCCTGTCAACAAGAA NAG 2 0.1481 Tier III
25 NC_000076.6 10 112011101 + Gm46179 NNNGGGCCTGTCAACAAGAA NAG 2 0.1481 Tier III
26 NC_000080.6 14 63099523 + Gm36025 NNNTGTCCTGTCAACAAGAA NTG 2 0.0142 Tier III
27 NC_000079.6 13 65767410 + Vmn2r-ps98 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier III
28 NC_000079.6 13 66158869 - Gm17514 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier III
29 NC_000079.6 13 66523686 - Gm46428 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier III
30 NC_000079.6 13 66593758 - Gm45966 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier III
31 NC_000079.6 13 66797533 + Gm7896 NNNGGTCCTGTCTACAAGAA NTG 2 0.0117 Tier III
32 NC_000073.6 7 86890747 - Gm39047 NNNGGTCCTGTCAAAAAGAA NTG 2 0.0087 Tier III
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Other clones with same target sequence:

(none)