Construct: sgRNA BRDN0001147141

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGATCAGCATTACTCCCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK4 (5596)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77223

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 50664500 + MAPK4 NNNTCAGCATTACTCCCACA NGG 0 1.0 Tier I
2 NC_000014.9 14 102247132 + MOK NNNTCAGCAACACTCCCACA NGG 2 0.5714 Tier II
3 NC_000023.11 X 13656245 + TCEANC NNNTCAGTATTACTCCCACT NGG 2 0.525 Tier II
4 NC_000019.10 19 35552744 - ATP4A NNNACAGCATCACTCCCACA NGG 2 0.3175 Tier II
5 NC_000013.11 13 33215253 - STARD13 NNNTCAGCATGACTCCAACA NGG 2 0.1867 Tier II
6 NC_000009.12 9 34572617 + CNTFR NNNTCATCATTACTCCCACA NAG 2 0.1481 Tier II
7 NC_000004.12 4 67962955 + TMPRSS11A NNNTCTGCATTAGTCCCACA NGG 2 0.0974 Tier II
8 NC_000006.12 6 47264143 - TNFRSF21 NNNTCAGCATTTCTCCCACA NAG 2 0.0864 Tier II
9 NC_000023.11 X 19090980 - ADGRG2 NNNTCAGCATCACTTCCACA NGG 2 0.0444 Tier II
10 NC_000004.12 4 123110170 - AFG2A NNNTCAGCATTACTTCCTCA NGG 2 0.0333 Tier II
11 NC_000008.11 8 47990474 - UBE2V2-AS1 NNNTCAGCAACACTCCCACA NGG 2 0.5714 Tier III
12 NC_000014.9 14 45381422 - LOC105370475 NNNTCATCATCACTCCCACA NGG 2 0.381 Tier III
13 NC_000008.11 8 2847427 - LOC105377785 NNNCCAGCAGTACTCCCACA NGG 2 0.3235 Tier III
14 NC_000006.12 6 44738787 - LOC101929770 NNNTCAGCATTGCTCTCACA NGG 2 0.2222 Tier III
15 NC_000009.12 9 34572617 + CNTFR-AS1 NNNTCATCATTACTCCCACA NAG 2 0.1481 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 73969896 - Mapk4 NNNTCAACATTACTCACACA NGG 2 1.0 Tier I
2 NC_000072.6 6 57210424 - Vmn1r13 NNNTCAGCATAACTCCCACA NGA 2 0.0521 Tier I
3 NC_000068.7 2 60700274 + Itgb6 NNNTCAGCCTTACTCACACA NGG 2 0.5714 Tier II
4 NC_000072.6 6 48414431 - Krba1 NNNTCAGCATCACTCCAACA NGG 2 0.3111 Tier II
5 NC_000085.6 19 3323010 + Cpt1a NNNTCAGCATAACTCCCGCA NGG 2 0.3 Tier II
6 NC_000070.6 4 117635536 + Eri3 NNNTCAGCATTACTCCCTTA NGG 2 0.2308 Tier II
7 NC_000079.6 13 97187646 + Hexb NNNTCACCATTTCTCCCACA NGG 2 0.2292 Tier II
8 NC_000074.6 8 16367468 + Csmd1 NNNTTAGCATTACTCTCACA NGG 2 0.1958 Tier II
9 NC_000076.6 10 123446269 - Tafa2 NNNTCAGTATTACTACCACA NGG 2 0.1944 Tier II
10 NC_000084.6 18 75505665 - Ctif NNNTCAGCAGTCCTCCCACA NGG 2 0.1316 Tier II
11 NC_000074.6 8 105180012 + Cbfb NNNTCAGCATTCCTACCACA NGG 2 0.0585 Tier II
12 NC_000069.6 3 59335154 - Igsf10 NNNTCAGCATTACCTCCACA NGG 2 0.019 Tier II
13 NC_000084.6 18 39003932 + Arhgap26 NNNTCTGCATTACTCCCACA NGC 2 0.0159 Tier II
14 NC_000068.7 2 60700274 + Gm33436 NNNTCAGCCTTACTCACACA NGG 2 0.5714 Tier III
15 NC_000070.6 4 106249964 - Gm12724 NNNTCAGGATTACACCCACA NGG 2 0.398 Tier III
16 NC_000071.6 5 39308329 - Gm40292 NNNCCATCATTACTCCCACA NGG 2 0.3697 Tier III
17 NC_000084.6 18 39003932 + Gm52330 NNNTCTGCATTACTCCCACA NGC 2 0.0159 Tier III
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Other clones with same target sequence:

(none)