Construct: sgRNA BRDN0001147142
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCCGAAACTCATGATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA1 (2041)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75590
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
143397947 |
+ |
EPHA1 |
NNNCCGAAACTCATGATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
69237364 |
- |
TJP2 |
NNNCCGAAACTCTTGAACAG |
NGG |
2 |
0.16 |
Tier II |
| 3 |
NC_000004.12 |
4 |
24136478 |
+ |
PPARGC1A |
NNNCCGAAAGTCACGATCAG |
NGG |
2 |
0.1111 |
Tier II |
| 4 |
NC_000011.10 |
11 |
64697600 |
+ |
NRXN2 |
NNNCCGAAACTCATGAAGAG |
NGG |
2 |
0.0711 |
Tier II |
| 5 |
NC_000023.11 |
X |
29387394 |
- |
IL1RAPL1 |
NNNCCAAAACTCATGATCAG |
NGT |
2 |
0.0161 |
Tier II |
| 6 |
NC_000017.11 |
17 |
10304669 |
- |
MYH13 |
NNNCAGAAACTCATGCTCAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000002.12 |
2 |
182361092 |
+ |
PDE1A |
NNNCCGAAACTAAGGATCAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000004.12 |
4 |
11746880 |
+ |
LINC02360 |
NNNCAAAAACTCATGATCAG |
NGG |
2 |
0.5714 |
Tier III |
| 9 |
NC_000004.12 |
4 |
11746880 |
+ |
LOC107986178 |
NNNCAAAAACTCATGATCAG |
NGG |
2 |
0.5714 |
Tier III |
| 10 |
NC_000005.10 |
5 |
139488649 |
+ |
LOC101059986 |
NNNCTGAAACTCATTATCAG |
NGG |
2 |
0.0909 |
Tier III |
| 11 |
NC_000017.11 |
17 |
10304669 |
- |
LOC107985004 |
NNNCAGAAACTCATGCTCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
42364188 |
+ |
Epha1 |
NNNCCGAAACTCATGGTCAG |
NGG |
1 |
0.1923 |
Tier I |
| 2 |
NC_000085.6 |
19 |
6960410 |
+ |
Plcb3 |
NNNCCCAAACTCAAGATCAG |
NGG |
2 |
0.4221 |
Tier II |
| 3 |
NC_000071.6 |
5 |
5583072 |
- |
Cfap69 |
NNNCCTAAACTTATGATCAG |
NGG |
2 |
0.359 |
Tier II |
| 4 |
NC_000079.6 |
13 |
57540331 |
+ |
Spock1 |
NNNCTGATACTCATGATCAG |
NGG |
2 |
0.2727 |
Tier II |
| 5 |
NC_000075.6 |
9 |
45507410 |
+ |
Dscaml1 |
NNNCCGAAACTCATGGTCAG |
NGA |
2 |
0.0134 |
Tier II |
| 6 |
NC_000074.6 |
8 |
9667287 |
+ |
Fam155a |
NNNCCGAAACTCATGATGAG |
NGA |
2 |
0.0093 |
Tier II |
Other clones with same target sequence:
(none)
