Construct: sgRNA BRDN0001147143
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGCCAGCCCTACCGAGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK1 (204851)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76993
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113941381 |
+ |
HIPK1 |
NNNCCAGCCCTACCGAGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
114646245 |
- |
ZBTB20 |
NNNCCAGTCCTACTGAGTGA |
NGG |
2 |
0.6417 |
Tier II |
| 3 |
NC_000016.10 |
16 |
75535087 |
+ |
CHST5 |
NNNCCAGCCCAACCGAGTGT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000016.10 |
16 |
70420861 |
+ |
ST3GAL2 |
NNNCCAGCCCTCCAGAGTGA |
NGG |
2 |
0.0921 |
Tier II |
| 5 |
NC_000011.10 |
11 |
85609499 |
- |
DLG2 |
NNNCAAGCCCTACCGAGTGA |
NTG |
2 |
0.0223 |
Tier II |
| 6 |
NC_000011.10 |
11 |
113314915 |
+ |
TTC12 |
NNNGCAGCCCTACCGAGTGA |
NGC |
2 |
0.0111 |
Tier II |
| 7 |
NC_000021.9 |
21 |
37564064 |
- |
LOC105372798 |
NNNCCAGCCCTACCGAGAGC |
NGG |
2 |
0.1515 |
Tier III |
| 8 |
NC_000021.9 |
21 |
37564064 |
- |
KCNJ6-AS1 |
NNNCCAGCCCTACCGAGAGC |
NGG |
2 |
0.1515 |
Tier III |
| 9 |
NC_000016.10 |
16 |
70420861 |
+ |
LOC124903707 |
NNNCCAGCCCTCCAGAGTGA |
NGG |
2 |
0.0921 |
Tier III |
| 10 |
NC_000001.11 |
1 |
66423156 |
+ |
LOC105378776 |
NNNCCAGCCCTACCAAGTGA |
NTG |
2 |
0.0367 |
Tier III |
| 11 |
NC_000006.12 |
6 |
233144 |
- |
LOC105374870 |
NNNCCAGCCCTACGGAGTGA |
NGC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
103777301 |
- |
Hipk1 |
NNNCCAACCCTACCGAGTGA |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
30474326 |
- |
Nphs1 |
NNNCCAGTCCTACCGAGTGA |
NGG |
1 |
0.875 |
Tier I |
| 3 |
NC_000076.6 |
10 |
81362855 |
- |
Mfsd12 |
NNNCCAGCCCTACCGTGTGT |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000077.6 |
11 |
9641422 |
- |
Abca13 |
NNNCCAACTCTACCGAGTGA |
NGG |
2 |
0.875 |
Tier II |
| 5 |
NC_000086.7 |
X |
144507499 |
- |
Trpc5 |
NNNCCAACCCTACTGAGTGA |
NGG |
2 |
0.7333 |
Tier II |
| 6 |
NC_000068.7 |
2 |
168533179 |
+ |
Nfatc2 |
NNNCCAGCCCGACCGAGTGG |
NGG |
2 |
0.3059 |
Tier II |
| 7 |
NC_000071.6 |
5 |
73407488 |
+ |
Cwh43 |
NNNCCAGCCCTGCCCAGTGA |
NGG |
2 |
0.197 |
Tier II |
| 8 |
NC_000071.6 |
5 |
116168958 |
- |
Ccdc60 |
NNNCCAGCCCTCCCCAGTGA |
NGG |
2 |
0.0718 |
Tier II |
| 9 |
NC_000086.7 |
X |
53075301 |
- |
Plac1 |
NNNCCAGCCCTACATAGTGA |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000081.6 |
15 |
58823549 |
- |
Tmem65 |
NNNGCAGCCCTACCGAGTGA |
NGC |
2 |
0.0111 |
Tier II |
| 11 |
NC_000073.6 |
7 |
110691981 |
- |
Gm21123 |
NNNCCAGCCCTACCAAATGA |
NGG |
2 |
0.8784 |
Tier III |
| 12 |
NC_000086.7 |
X |
53075301 |
- |
Gm35988 |
NNNCCAGCCCTACATAGTGA |
NGG |
2 |
0.05 |
Tier III |
| 13 |
NC_000080.6 |
14 |
31449138 |
+ |
Gm35907 |
NNNCCAGCCCTTCCTAGTGA |
NGG |
2 |
0.0476 |
Tier III |
| 14 |
NC_000077.6 |
11 |
6656235 |
+ |
Gm11978 |
NNNCCAGCCCTACCGAGTGC |
NGA |
2 |
0.0158 |
Tier III |
Other clones with same target sequence:
(none)
