Construct: sgRNA BRDN0001147144
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCATGACCAAATATGCTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BAZ1B (9031)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76134
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
73508352 |
- |
BAZ1B |
NNNATGACCAAATATGCTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
7900043 |
- |
MTRR |
NNNATGACCAAATATCCTGA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000004.12 |
4 |
168873393 |
- |
PALLD |
NNNATGAATAAATATGCTGT |
NGG |
2 |
0.5688 |
Tier II |
| 4 |
NC_000002.12 |
2 |
224862420 |
- |
DOCK10 |
NNNATGACCAGATATGCTAT |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000023.11 |
X |
17414375 |
- |
NHS |
NNNATGACCAAACATGCTGA |
NGG |
2 |
0.4441 |
Tier II |
| 6 |
NC_000014.9 |
14 |
38212856 |
+ |
SSTR1 |
NNNATGAACAAATATGCTTT |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000019.10 |
19 |
11733415 |
+ |
ZNF823 |
NNNATGACCAAATATGTTTT |
NGG |
2 |
0.3111 |
Tier II |
| 8 |
NC_000010.11 |
10 |
6297041 |
+ |
PFKFB3 |
NNNATGACCAACCATGCTGT |
NGG |
2 |
0.2078 |
Tier II |
| 9 |
NC_000008.11 |
8 |
138251705 |
- |
FAM135B |
NNNATCACCAAAGATGCTGT |
NGG |
2 |
0.1779 |
Tier II |
| 10 |
NC_000017.11 |
17 |
1037503 |
- |
ABR |
NNNATGACCAAATGTGCTGT |
NAG |
2 |
0.121 |
Tier II |
| 11 |
NC_000002.12 |
2 |
127309597 |
- |
MAP3K2 |
NNNATGACTAAATATGGTGT |
NGG |
2 |
0.0515 |
Tier II |
| 12 |
NC_000002.12 |
2 |
206967699 |
+ |
CPO |
NNNATGACAAAATATGGTGT |
NGG |
2 |
0.0504 |
Tier II |
| 13 |
NC_000002.12 |
2 |
38336395 |
+ |
ATL2 |
NNNATTACCAAATATGCTGT |
NGA |
2 |
0.0463 |
Tier II |
| 14 |
NC_000005.10 |
5 |
39393453 |
+ |
DAB2 |
NNNATGACCAAATATGCTCT |
NGA |
2 |
0.0311 |
Tier II |
| 15 |
NC_000004.12 |
4 |
176225041 |
+ |
ASB5 |
NNNATGAACAAATATGCTGT |
NTG |
2 |
0.0253 |
Tier II |
| 16 |
NC_000007.14 |
7 |
89140124 |
+ |
ZNF804B |
NNNATGACCAAATATGCTGT |
NAA |
2 |
0.0 |
Tier II |
| 17 |
NC_000004.12 |
4 |
168873393 |
- |
LOC102723331 |
NNNATGAATAAATATGCTGT |
NGG |
2 |
0.5688 |
Tier III |
| 18 |
NC_000004.12 |
4 |
44018743 |
- |
LINC02475 |
NNNATGGGCAAATATGCTGT |
NGG |
2 |
0.4538 |
Tier III |
| 19 |
NC_000005.10 |
5 |
152637117 |
- |
LINC01470 |
NNNATGACCACATATGCTTT |
NGG |
2 |
0.2667 |
Tier III |
| 20 |
NC_000010.11 |
10 |
125486109 |
+ |
LOC105378543 |
NNNAAGACCAAATGTGCTGT |
NGG |
2 |
0.2333 |
Tier III |
| 21 |
NC_000016.10 |
16 |
52610051 |
- |
LINC03064 |
NNNATGACCTACTATGCTGT |
NGG |
2 |
0.2322 |
Tier III |
| 22 |
NC_000011.10 |
11 |
25799720 |
+ |
LINC02699 |
NNNATGACCAAATAAGCTGT |
NTG |
2 |
0.0226 |
Tier III |
| 23 |
NC_000008.11 |
8 |
23665566 |
- |
LOC107986930 |
NNNATGACCAAATGTGCTGT |
NGT |
2 |
0.0075 |
Tier III |
| 24 |
NC_000021.9 |
21 |
28098086 |
- |
LINC01697 |
NNNATGGCCAAATATTCTGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
135198291 |
+ |
Baz1b |
NNNATGACCAAATATGCCGT |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000067.6 |
1 |
34548419 |
+ |
Prss40 |
NNNATCACCAAATATGCTTT |
NGG |
2 |
0.4545 |
Tier II |
| 3 |
NC_000072.6 |
6 |
49055163 |
+ |
Gpnmb |
NNNATGACCAGATATGCTTT |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000082.6 |
16 |
72898847 |
- |
Robo1 |
NNNATGACCAGATATGCTTT |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000086.7 |
X |
53297496 |
+ |
Fam122c |
NNNATGAGGAAATATGCTGT |
NGG |
2 |
0.398 |
Tier II |
| 6 |
NC_000082.6 |
16 |
49174644 |
+ |
Myh15 |
NNNAAGAACAAATATGCTGT |
NGG |
2 |
0.325 |
Tier II |
| 7 |
NC_000082.6 |
16 |
11521843 |
- |
Snx29 |
NNNATGACCACATATGCTAT |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000069.6 |
3 |
24690368 |
- |
Naaladl2 |
NNNAGGCCCAAATATGCTGT |
NGG |
2 |
0.28 |
Tier II |
| 9 |
NC_000068.7 |
2 |
155523055 |
- |
Acss2 |
NNNATGACCAAATATACTGT |
NAG |
2 |
0.2593 |
Tier II |
| 10 |
NC_000075.6 |
9 |
9952896 |
- |
Cntn5 |
NNNATGAACATATATGCTGT |
NGG |
2 |
0.2 |
Tier II |
| 11 |
NC_000079.6 |
13 |
9125824 |
+ |
Larp4b |
NNNATGAGCAAAGATGCTGT |
NGG |
2 |
0.1677 |
Tier II |
| 12 |
NC_000069.6 |
3 |
35800963 |
+ |
Atp11b |
NNNATGACCAAATATAGTGT |
NGG |
2 |
0.0588 |
Tier II |
| 13 |
NC_000070.6 |
4 |
77702294 |
- |
Ptprd |
NNNATGACCAAATATGCTGT |
NGT |
1 |
0.0161 |
Tier II |
| 14 |
NC_000084.6 |
18 |
45889109 |
- |
A330093E20Rik |
NNNATGAACAAGTATGCTGT |
NGG |
2 |
0.4694 |
Tier III |
| 15 |
NC_000068.7 |
2 |
69380112 |
- |
Gm36393 |
NNNATGACCAAATATGGTTT |
NGG |
2 |
0.0392 |
Tier III |
| 16 |
NC_000073.6 |
7 |
4177007 |
+ |
Gm5319 |
NNNATGAACAAATATTCTGT |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000081.6 |
15 |
25415726 |
+ |
Gm48957 |
NNNATGACCAAATATCCTCT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
