Construct: sgRNA BRDN0001147147
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGCTTCCCTTATGCCCGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1L1 (221823)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75841
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
18027497 |
- |
PRPS1L1 |
NNNCTTCCCTTATGCCCGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
107639459 |
+ |
PRPS1 |
NNNCTTCCCTTATGCCCGGC |
NGG |
1 |
0.375 |
Tier I |
| 3 |
NC_000011.10 |
11 |
130885250 |
+ |
SNX19 |
NNNCTTCCCTTATGCCAGAC |
NGG |
1 |
0.4667 |
Tier II |
| 4 |
NC_000006.12 |
6 |
34045096 |
+ |
GRM4 |
NNNCTTCCCTTGTGCCCCAC |
NGG |
2 |
0.3439 |
Tier II |
| 5 |
NC_000005.10 |
5 |
94307291 |
+ |
KIAA0825 |
NNNCTTCCCTTGTGCCTGAC |
NGG |
2 |
0.337 |
Tier II |
| 6 |
NC_000009.12 |
9 |
125150934 |
+ |
PPP6C |
NNNCTTCCCTTATGCCCAGC |
NGG |
2 |
0.2596 |
Tier II |
| 7 |
NC_000001.11 |
1 |
44243306 |
- |
ERI3 |
NNNCTTCCCTGATGCCCGGC |
NGG |
2 |
0.15 |
Tier II |
| 8 |
NC_000002.12 |
2 |
158692517 |
- |
PKP4-AS1 |
NNNCTTCTCTTATGCCAGAC |
NGG |
2 |
0.4083 |
Tier III |
| 9 |
NC_000009.12 |
9 |
125150934 |
+ |
PRPS1P2 |
NNNCTTCCCTTATGCCCAGC |
NGG |
2 |
0.2596 |
Tier III |
| 10 |
NC_000001.11 |
1 |
44243306 |
- |
ERI3-IT1 |
NNNCTTCCCTGATGCCCGGC |
NGG |
2 |
0.15 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
34985174 |
+ |
Prps1l1 |
NNNCTTCCCTTATGCCCGGC |
NGG |
1 |
0.375 |
Tier I |
| 2 |
NC_000078.6 |
12 |
57238712 |
+ |
Prps1l3 |
NNNCTTCCCTTATGCCCGCC |
NAG |
2 |
0.0536 |
Tier I |
| 3 |
NC_000078.6 |
12 |
57238712 |
+ |
Mipol1 |
NNNCTTCCCTTATGCCCGCC |
NAG |
2 |
0.0536 |
Tier II |
| 4 |
NC_000084.6 |
18 |
29435852 |
+ |
4930578E11Rik |
NNNCTCCCCTTATGCCCTAC |
NGG |
2 |
0.6061 |
Tier III |
| 5 |
NC_000078.6 |
12 |
34985174 |
+ |
Gm33864 |
NNNCTTCCCTTATGCCCGGC |
NGG |
1 |
0.375 |
Tier III |
| 6 |
NC_000083.6 |
17 |
4190133 |
- |
Gm41516 |
NNNCTTCCCTTATCCCTGAC |
NGG |
2 |
0.2 |
Tier III |
| 7 |
NC_000079.6 |
13 |
118704139 |
+ |
Gm16263 |
NNNCTTCCCTTATGCCAGAC |
NTG |
2 |
0.0182 |
Tier III |
Other clones with same target sequence:
(none)
