Construct: sgRNA BRDN0001147148

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAAAATGAGCCATCCATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIK2 (23235)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77140

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 111703353 + SIK2 NNNAAATGAGCCATCCATCG NGG 0 1.0 Tier I
2 NC_000011.10 11 111703353 + PPP2R1B NNNAAATGAGCCATCCATCG NGG 0 1.0 Tier II
3 NC_000001.11 1 173989277 + RC3H1 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier II
4 NC_000001.11 1 173989437 + RC3H1 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier II
5 NC_000007.14 7 134036358 - EXOC4 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier II
6 NC_000011.10 11 113271140 - NCAM1 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier II
7 NC_000001.11 1 180039079 + CEP350 NNNAAATTAGCCATCCACCG NGG 2 0.2679 Tier II
8 NC_000002.12 2 162444074 + KCNH7 NNNAAATTAGCCATCCATCC NGG 2 0.2679 Tier II
9 NC_000003.12 3 124442888 - KALRN NNNAAATGAGCCTTCCATCT NGG 2 0.21 Tier II
10 NC_000009.12 9 130818332 + ABL1 NNNAAATTAGCCATACATCG NGG 2 0.1389 Tier II
11 NC_000016.10 16 17418710 + XYLT1 NNNAAATAAGCCATCCATGG NGG 2 0.125 Tier II
12 NC_000003.12 3 17606305 + TBC1D5 NNNAAATTAGCCATCCATGG NGG 2 0.0781 Tier II
13 NC_000016.10 16 84015862 - SLC38A8 NNNAAATTAGCCATCCATGG NGG 2 0.0781 Tier II
14 NC_000023.11 X 45194406 - DIPK2B NNNAAATTAGCCATCCATGG NGG 2 0.0781 Tier II
15 NC_000001.11 1 108973746 + WDR47 NNNAAATGTGCCATCCATGG NGG 2 0.075 Tier II
16 NC_000002.12 2 32642012 - TTC27 NNNAGATGAGCCATGCATCG NGG 2 0.036 Tier II
17 NC_000010.11 10 92599387 + KIF11 NNNAAATTAGCCATGCATCG NGG 2 0.0313 Tier II
18 NC_000010.11 10 102832230 - CYP17A1 NNNAAATTAGCCATGCATCG NGG 2 0.0313 Tier II
19 NC_000021.9 21 36899967 + HLCS NNNAAATTAGCCATGCATCG NGG 2 0.0313 Tier II
20 NC_000003.12 3 9784727 + TADA3 NNNAAATGAGCCATACATGG NGG 2 0.0278 Tier II
21 NC_000010.11 10 15032485 + OLAH NNNAAATGAGCTATGCATCG NGG 2 0.0269 Tier II
22 NC_000005.10 5 167449820 + TENM2 NNNAAATGAGCCATGCATAG NGG 2 0.0214 Tier II
23 NC_000004.12 4 37530436 + C4orf19 NNNAAATGAGCCATGCATGG NGG 2 0.0063 Tier II
24 NC_000014.9 14 102656932 - RCOR1 NNNAAATGAGCCATGCATGG NGG 2 0.0063 Tier II
25 NC_000018.10 18 46853139 + PIAS2 NNNAAATGAGCCAGCCATAG NGG 2 0.0 Tier II
26 NC_000001.11 1 93162459 + TMED5 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
27 NC_000001.11 1 169877311 - SCYL3 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
28 NC_000001.11 1 228395678 - TRIM11 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
29 NC_000008.11 8 84710458 - RALYL NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
30 NC_000009.12 9 92222179 + IARS1 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
31 NC_000010.11 10 96243409 + BLNK NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
32 NC_000012.12 12 109228381 + ACACB NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
33 NC_000013.11 13 32597190 - PDS5B NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
34 NC_000015.10 15 65777030 + DENND4A NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
35 NC_000016.10 16 70489374 - COG4 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
36 NC_000010.11 10 14542132 + FAM107B NNNAAATGAGCCAGGCATCG NGG 2 0.0 Tier II
37 NC_000009.12 9 125265568 - GAPVD1 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier II
38 NC_000019.10 19 33136719 - WDR88 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier II
39 NC_000011.10 11 113271140 - NCAM1-AS1 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier III
40 NC_000013.11 13 95369865 - LOC124903193 NNNAAATGATCCATCCATCT NGG 2 0.5688 Tier III
41 NC_000007.14 7 20017631 + LOC101927668 NNNAAATGAGCCATCCACCT NGG 2 0.3 Tier III
42 NC_000001.11 1 106062338 - LOC105378885 NNNAAATGAGACATCCATGG NGG 2 0.0938 Tier III
43 NC_000009.12 9 62458030 + LINC01189 NNNAAATTAGCCATCCATGG NGG 2 0.0781 Tier III
44 NC_000009.12 9 62458030 + LOC128966771 NNNAAATTAGCCATCCATGG NGG 2 0.0781 Tier III
45 NC_000010.11 10 15032485 + DCLRE1CP1 NNNAAATGAGCTATGCATCG NGG 2 0.0269 Tier III
46 NC_000010.11 10 15032485 + ACBD7-DCLRE1CP1 NNNAAATGAGCTATGCATCG NGG 2 0.0269 Tier III
47 NC_000018.10 18 46853139 + LOC100421737 NNNAAATGAGCCAGCCATAG NGG 2 0.0 Tier III
48 NC_000001.11 1 150916293 - LOC107985204 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier III
49 NC_000009.12 9 87276221 + LOC105376126 NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier III
50 NC_000003.12 3 73147787 - LOC107986098 NNNAAATGAGCCAGGCATCG NGG 2 0.0 Tier III
51 NC_000007.14 7 117458699 + LOC105375468 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier III
52 NC_000009.12 9 38869702 + LOC105376043 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier III
53 NC_000009.12 9 62094129 + LOC107987008 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier III
54 NC_000009.12 9 67494253 - LOC105379452 NNNAAATTAGCCAGCCATCG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 136528864 + Cux1 NNNAAATGAACCATCCATCA NGG 2 0.875 Tier II
2 NC_000077.6 11 84158591 + Acaca NNNAAATGGACCATCCATCG NGG 2 0.6222 Tier II
3 NC_000077.6 11 84158591 + Gm11437 NNNAAATGGACCATCCATCG NGG 2 0.6222 Tier II
4 NC_000067.6 1 54679889 - Ankrd44 NNNAAATGAGCCATCCATCT NAG 2 0.1815 Tier II
5 NC_000079.6 13 68635595 - Adcy2 NNNAAATGAGCCATCCATCT NAG 2 0.1815 Tier II
6 NC_000071.6 5 96813210 + Anxa3 NNNAAATGACCCATCCATAG NGG 2 0.1714 Tier II
7 NC_000072.6 6 45200370 - Cntnap2 NNNAAATGAACCATCCATGG NGG 2 0.1167 Tier II
8 NC_000068.7 2 65940159 + Csrnp3 NNNAAATGAGCCATCCCTCC NGG 2 0.0756 Tier II
9 NC_000070.6 4 83027946 - Frem1 NNNAAATGAGCCATTCATCA NGG 2 0.0625 Tier II
10 NC_000067.6 1 123980518 - Dpp10 NNNAACTGAGCCATCCTTCG NGG 2 0.0606 Tier II
11 NC_000068.7 2 80142395 - Pde1a NNNAAATGAGCCATCCATCG NGC 1 0.0222 Tier II
12 NC_000074.6 8 105974378 - Dpep3 NNNAAATGAGCCTTCCATCG NGA 2 0.0208 Tier II
13 NC_000068.7 2 135112435 - Plcb1 NNNAAATGAGCCATTCATGG NGG 2 0.0083 Tier II
14 NC_000077.6 11 47951909 - Sgcd NNNAAATGAGCCAGCCATGG NGG 2 0.0 Tier II
15 NC_000072.6 6 82839694 + Gm32591 NNNAAACAAGCCATCCATCG NGG 2 0.6875 Tier III
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Other clones with same target sequence:

(none)