Construct: sgRNA BRDN0001147153
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGGAGAGACTCACCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK5 (8550)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76448
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
111885951 |
+ |
MAPKAPK5 |
NNNGGAGAGACTCACCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
66558272 |
+ |
GRIP1 |
NNNGGAGAGACTCACAATCA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000009.12 |
9 |
37878753 |
+ |
SLC25A51 |
NNNAGAGACACTCACCATCG |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000003.12 |
3 |
76859618 |
+ |
ROBO2 |
NNNGGAGAGCCCCACCATCG |
NGG |
2 |
0.3158 |
Tier II |
| 5 |
NC_000013.11 |
13 |
66671187 |
- |
PCDH9 |
NNNGGGGAGCCTCACCATCG |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000016.10 |
16 |
12563329 |
+ |
SNX29 |
NNNGGAGAGAGTCACCGTCG |
NGG |
2 |
0.0441 |
Tier II |
| 7 |
NC_000010.11 |
10 |
60652943 |
- |
ANK3 |
NNNGGAGAGGCTCACCATCG |
NGA |
2 |
0.0386 |
Tier II |
| 8 |
NC_000011.10 |
11 |
133181870 |
- |
OPCML |
NNNGGAGGGACTCAGCATCG |
NGG |
2 |
0.0367 |
Tier II |
| 9 |
NC_000014.9 |
14 |
61217206 |
- |
PRKCH |
NNNGGAGAGACTCACCATCT |
NGT |
2 |
0.0113 |
Tier II |
| 10 |
NC_000009.12 |
9 |
37878753 |
+ |
PAICSP1 |
NNNAGAGACACTCACCATCG |
NGG |
2 |
0.4846 |
Tier III |
| 11 |
NC_000016.10 |
16 |
12563329 |
+ |
LOC105371085 |
NNNGGAGAGAGTCACCGTCG |
NGG |
2 |
0.0441 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
121528956 |
- |
Mapkapk5 |
NNNGGAAAGACTCACAATCG |
NGG |
2 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
96988610 |
- |
Spsb4 |
NNNAGAGAGACTCACCATTG |
NGG |
2 |
0.4154 |
Tier II |
| 3 |
NC_000084.6 |
18 |
22701441 |
- |
Nol4 |
NNNGGAGAGTCTCACCATTG |
NGG |
2 |
0.4072 |
Tier II |
| 4 |
NC_000083.6 |
17 |
27470891 |
+ |
Grm4 |
NNNTGACAGACTCACCATCG |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000081.6 |
15 |
66671604 |
+ |
Tg |
NNNGGAGAGACTCTCCATTG |
NGG |
2 |
0.2462 |
Tier II |
| 6 |
NC_000074.6 |
8 |
87469712 |
- |
Cbln1 |
NNNGGAAAGACTCACCTTCG |
NGG |
2 |
0.1333 |
Tier II |
| 7 |
NC_000074.6 |
8 |
16409927 |
- |
Csmd1 |
NNNGGAGAGCCTCAGCATCG |
NGG |
2 |
0.0167 |
Tier II |
Other clones with same target sequence:
(none)
