Construct: sgRNA BRDN0001147156
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGCCTTCAAACTAGACCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK12 (51755)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75915
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
39462348 |
+ |
CDK12 |
NNNCCTTCAAACTAGACCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
14620599 |
- |
PARN |
NNNCCTTCAATCTAGACTGA |
NGG |
2 |
0.1978 |
Tier II |
| 3 |
NC_000013.11 |
13 |
50090704 |
+ |
DLEU2 |
NNNCCTTCAAACTAGACCTG |
NGG |
2 |
0.5098 |
Tier III |
| 4 |
NC_000013.11 |
13 |
50090704 |
+ |
DLEU1 |
NNNCCTTCAAACTAGACCTG |
NGG |
2 |
0.5098 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
104805005 |
- |
Mov10 |
NNNTCTTCAAACTAGACCGC |
NGG |
2 |
0.1818 |
Tier I |
| 2 |
NC_000067.6 |
1 |
134047470 |
- |
Fmod |
NNNCCTTCATACTAGACCAA |
NGG |
2 |
0.6303 |
Tier II |
| 3 |
NC_000069.6 |
3 |
27970129 |
- |
Pld1 |
NNNCCTTCAAACTAAACCGA |
NAG |
2 |
0.244 |
Tier II |
| 4 |
NC_000077.6 |
11 |
87338830 |
- |
Ppm1e |
NNNCCTTGAAACTAGAGCGA |
NGG |
2 |
0.0378 |
Tier II |
| 5 |
NC_000071.6 |
5 |
141789872 |
+ |
Sdk1 |
NNNCCTTCAAACTAGCCAGA |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000068.7 |
2 |
156664731 |
- |
Dlgap4 |
NNNCCTTCAAACTAGCCCCA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000067.6 |
1 |
4523154 |
+ |
Gm7357 |
NNNTCTTCAAACTAGACCGC |
NGG |
2 |
0.1818 |
Tier III |
Other clones with same target sequence:
(none)
