Construct: sgRNA BRDN0001147157
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCACGGAGAAACCCTCGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA2 (1969)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76368
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
16135158 |
+ |
EPHA2 |
NNNCGGAGAAACCCTCGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
73537692 |
+ |
ACOT1 |
NNNCCGAGAAACCCTTGGTG |
NGG |
2 |
0.2418 |
Tier I |
| 3 |
NC_000014.9 |
14 |
73537692 |
+ |
HEATR4 |
NNNCCGAGAAACCCTTGGTG |
NGG |
2 |
0.2418 |
Tier II |
| 4 |
NC_000016.10 |
16 |
57727507 |
+ |
DRC7 |
NNNCTGAGAAACCCTCTGTG |
NGG |
2 |
0.075 |
Tier II |
| 5 |
NC_000009.12 |
9 |
131487585 |
- |
PRRC2B |
NNNCGGAGAAGCCCGCGGTG |
NGG |
2 |
0.0325 |
Tier II |
| 6 |
NC_000024.10 |
Y |
21468824 |
+ |
TSPY14P |
NNNCAGAGAAACCCTCAGTG |
NGG |
2 |
0.8089 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
49608638 |
- |
Nav2 |
NNNCAGAGACACCCTCGGTG |
NGG |
2 |
0.2889 |
Tier II |
| 2 |
NC_000068.7 |
2 |
31068161 |
+ |
Fnbp1 |
NNNCAGAGAAACCCTGGGTG |
NGG |
2 |
0.1333 |
Tier II |
| 3 |
NC_000082.6 |
16 |
90782002 |
+ |
Urb1 |
NNNCTGAGAAAGCCTCGGTG |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000071.6 |
5 |
121924064 |
- |
Cux2 |
NNNCTGAGACACCCTCGGTG |
NGG |
2 |
0.1 |
Tier II |
| 5 |
NC_000080.6 |
14 |
17777448 |
+ |
Thrb |
NNNCGGAGAAACCCTGGGTG |
NGT |
2 |
0.0025 |
Tier II |
Other clones with same target sequence:
(none)
