Construct: sgRNA BRDN0001147158

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCCTCCAGTCACGGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT4 (2324)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75629

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 180624028 + FLT4 NNNCTCCAGTCACGGCACTG NGG 0 1.0 Tier I
2 NC_000009.12 9 127575110 + NIBAN2 NNNCTCCAGACATGGCACTG NGG 2 0.6 Tier II
3 NC_000023.11 X 119006350 + LONRF3 NNNCCCCAGCCACGGCACTG NGG 2 0.5333 Tier II
4 NC_000011.10 11 1854594 - LSP1 NNNCTCCACTCACGGCACTC NGG 2 0.2308 Tier II
5 NC_000015.10 15 68406700 - ITGA11 NNNCTCCATTCAAGGCACTG NGG 2 0.2051 Tier II
6 NC_000015.10 15 32072716 + CHRNA7 NNNCTCCAGTCATGGCACAG NGG 2 0.2 Tier II
7 NC_000016.10 16 87858506 + SLC7A5 NNNCTCCAGTCACGGTATTG NGG 2 0.1978 Tier II
8 NC_000012.12 12 103817962 + NT5DC3 NNNCTCCAGTCAGGGCACTG NGG 1 0.1364 Tier II
9 NC_000002.12 2 127367520 + MAP3K2 NNNCTCCAGGCAGGGCACTG NGG 2 0.0682 Tier II
10 NC_000006.12 6 160487109 + LPAL2 NNNCCCCAGACACGGCACTG NGG 2 0.8571 Tier III
11 NC_000010.11 10 3421113 - LOC105376360 NNNCTCCAGGCACAGCACTG NGG 2 0.375 Tier III
12 NC_000016.10 16 29602670 + SMG1P2 NNNCTCCAGTCACGGTATTG NGG 2 0.1978 Tier III
13 NC_000016.10 16 29602670 + LOC105371168 NNNCTCCAGTCACGGTATTG NGG 2 0.1978 Tier III
14 NC_000002.12 2 127367520 + LOC124906074 NNNCTCCAGGCAGGGCACTG NGG 2 0.0682 Tier III
15 NC_000017.11 17 76950600 - LOC105371899 NNNCTGCAGTCACGGCTCTG NGG 2 0.0667 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 49632013 - Flt4 NNNTTCCAGTCTCGGCACTG NGG 2 0.2667 Tier I
2 NC_000068.7 2 24623987 + Cacna1b NNNCTCCAGACACGGCACTG NGG 1 0.8571 Tier II
3 NC_000077.6 11 76524817 - Abr NNNCTCCAGTCATGGAACTG NGG 2 0.7 Tier II
4 NC_000074.6 8 117733837 + Hsd17b2 NNNCTACAGTCACAGCACTG NGG 2 0.6964 Tier II
5 NC_000079.6 13 100100361 + Bdp1 NNNCTCTAGTCACAGCACTG NGG 2 0.6094 Tier II
6 NC_000072.6 6 120874482 + Bcl2l13 NNNCTCCAGGCACAGCACTG NGG 2 0.375 Tier II
7 NC_000086.7 X 73523942 + Atp2b3 NNNCTGCAGTCGCGGCACTG NGG 2 0.3611 Tier II
8 NC_000067.6 1 38821931 + Lonrf2 NNNCTGCAGTCATGGCACTG NGG 2 0.35 Tier II
9 NC_000077.6 11 108115691 + Prkca NNNCTCCAGTCACAGCACTC NGG 2 0.3214 Tier II
10 NC_000076.6 10 94189640 - Nr2c1 NNNCTCCAATCACCGCACTG NGG 2 0.2755 Tier II
11 NC_000081.6 15 99795311 + Lima1 NNNCTCCAGTCCCAGCACTG NGG 2 0.1974 Tier II
12 NC_000081.6 15 31232557 + Dap NNNCTCCAGACACGGCCCTG NGG 2 0.1513 Tier II
13 NC_000072.6 6 86394619 + Pcyox1 NNNCTCCAGACACGGCTCTG NGG 2 0.1143 Tier II
14 NC_000072.6 6 136697768 + Gucy2c NNNTTCCAGTCAGGGCACTG NGG 2 0.1091 Tier II
15 NC_000074.6 8 13624207 - Rasa3 NNNCTCCAGGCAGGGCACTG NGG 2 0.0682 Tier II
16 NC_000068.7 2 9915909 - Taf3 NNNCTCCAGTCACAGCACTG NGA 2 0.0521 Tier II
17 NC_000068.7 2 157447236 - Src NNNCTCCAGTCAGGGCAGTG NGG 2 0.0182 Tier II
18 NC_000080.6 14 44954173 + Gm16976 NNNCCCCAGTCACGGAACTG NGG 2 1.0 Tier III
19 NC_000080.6 14 44954173 + Gm41142 NNNCCCCAGTCACGGAACTG NGG 2 1.0 Tier III
20 NC_000075.6 9 7682214 - AV064505 NNNCTCCGGTCACGGCACTG NGG 1 0.7333 Tier III
21 NC_000077.6 11 108115691 + Gm51922 NNNCTCCAGTCACAGCACTC NGG 2 0.3214 Tier III
22 NC_000069.6 3 40426924 + Gm30184 NNNCCCCAGTCACTGCACTG NGG 2 0.2667 Tier III
23 NC_000068.7 2 130903480 - A730017L22Rik NNNCTCCAGTCCCAGCACTG NGG 2 0.1974 Tier III
24 NC_000074.6 8 87167009 - Gm27246 NNNCTCCAGTGACAGCACTG NGG 2 0.1875 Tier III
25 NC_000077.6 11 71565442 - 9230020A06Rik NNNCTCCAGTCACTGCAATG NGG 2 0.1436 Tier III
26 NC_000077.6 11 71565442 - Gm33351 NNNCTCCAGTCACTGCAATG NGG 2 0.1436 Tier III
27 NC_000075.6 9 64774584 + Gm36365 NNNCTCCAGTCACGGGGCTG NGG 2 0.0271 Tier III
Download CSV

Other clones with same target sequence:

(none)