Construct: sgRNA BRDN0001147167
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAACAAGGGGTGCCAACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OXSR1 (9943)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77751
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
38221647 |
- |
OXSR1 |
NNNACAAGGGGTGCCAACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
153409657 |
+ |
PNMA6E |
NNNACACGCGGTGCCAACAA |
NGG |
2 |
0.2356 |
Tier II |
| 3 |
NC_000016.10 |
16 |
56937205 |
+ |
HERPUD1 |
NNNACAATGGGTGACAACAA |
NGG |
2 |
0.2188 |
Tier II |
| 4 |
NC_000016.10 |
16 |
28606882 |
+ |
SULT1A1 |
NNNGCAAGGGGTGCCAACAC |
NGG |
2 |
0.142 |
Tier II |
| 5 |
NC_000011.10 |
11 |
41403545 |
+ |
LRRC4C |
NNNACAAGGGGTCCCAACAA |
NAG |
2 |
0.1092 |
Tier II |
| 6 |
NC_000019.10 |
19 |
13928308 |
- |
CC2D1A |
NNNAAAAGGGGTGCCAAGAA |
NGG |
2 |
0.0762 |
Tier II |
| 7 |
NC_000015.10 |
15 |
51715445 |
- |
SCG3 |
NNNACAAAGGGTGGCAACAA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000006.12 |
6 |
99474682 |
+ |
USP45 |
NNNACAAGGGCTGCCCACAA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000006.12 |
6 |
99474682 |
+ |
TSTD3 |
NNNACAAGGGCTGCCCACAA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
48969341 |
+ |
Lrfn2 |
NNNACAAGGGATGCCAACAG |
NGG |
2 |
0.7647 |
Tier II |
| 2 |
NC_000077.6 |
11 |
94996782 |
- |
Ppp1r9b |
NNNACAAGGGATGCCAAGAA |
NGG |
2 |
0.1333 |
Tier II |
| 3 |
NC_000070.6 |
4 |
128768808 |
+ |
A3galt2 |
NNNACAAGGGGTGCCAGCAT |
NGG |
2 |
0.1059 |
Tier II |
| 4 |
NC_000069.6 |
3 |
152099728 |
+ |
Gipc2 |
NNNACAAGGGCTGCCAACAC |
NGG |
2 |
0.0974 |
Tier II |
| 5 |
NC_000077.6 |
11 |
70051492 |
- |
Asgr1 |
NNNACAAGGGGTGCCAAGGA |
NGG |
2 |
0.05 |
Tier II |
| 6 |
NC_000078.6 |
12 |
41574572 |
+ |
Immp2l |
NNNACATGGGGTGCCAACAA |
NGT |
2 |
0.0071 |
Tier II |
| 7 |
NC_000071.6 |
5 |
105640508 |
+ |
Gm32230 |
NNNACAATGGCTGCCAACAA |
NGG |
2 |
0.2679 |
Tier III |
| 8 |
NC_000069.6 |
3 |
152099728 |
+ |
Gm31881 |
NNNACAAGGGCTGCCAACAC |
NGG |
2 |
0.0974 |
Tier III |
| 9 |
NC_000076.6 |
10 |
25596049 |
- |
Gm29571 |
NNNACAAGGAGTGCCAACAA |
NGA |
2 |
0.0648 |
Tier III |
| 10 |
NC_000085.6 |
19 |
7844229 |
+ |
Gm7096 |
NNNACAAGTGGTGCCAACAA |
NGT |
2 |
0.0086 |
Tier III |
Other clones with same target sequence:
(none)
