Construct: sgRNA BRDN0001147168
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTTGAGGGATACCATATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FLT1 (2321)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75559
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28427226 |
+ |
FLT1 |
NNNTTGAGGGATACCATATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
123573393 |
- |
CSNK1G3 |
NNNTTGAGGTATACCATCTG |
NGG |
2 |
0.1548 |
Tier I |
| 3 |
NC_000021.9 |
21 |
34985633 |
+ |
RUNX1 |
NNNTTTGGGGATACCATATG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000001.11 |
1 |
241585355 |
- |
KMO |
NNNGTGAGAGATACCATATG |
NGG |
2 |
0.4018 |
Tier II |
| 5 |
NC_000003.12 |
3 |
64263888 |
- |
PRICKLE2 |
NNNTTGAGGGAGACCACATG |
NGG |
2 |
0.3529 |
Tier II |
| 6 |
NC_000011.10 |
11 |
93820110 |
+ |
VSTM5 |
NNNTTGTGGGATACCAGATG |
NGG |
2 |
0.0515 |
Tier II |
| 7 |
NC_000023.11 |
X |
46605848 |
- |
SLC9A7 |
NNNTTGAGGGCTACCATATG |
NGC |
2 |
0.0089 |
Tier II |
| 8 |
NC_000001.11 |
1 |
46677880 |
+ |
EFCAB14 |
NNNTTGAGGGATACCTTATG |
NAG |
2 |
0.0 |
Tier II |
| 9 |
NC_000008.11 |
8 |
113149758 |
- |
CSMD3 |
NNNTTGAGGGATAGCATATG |
NGA |
2 |
0.0 |
Tier II |
| 10 |
NC_000001.11 |
1 |
34519161 |
- |
LOC105378641 |
NNNTTGAGGGATCCCATATA |
NGG |
2 |
0.1974 |
Tier III |
| 11 |
NC_000014.9 |
14 |
39641565 |
+ |
LOC105370461 |
NNNTTGAGGGATGCCATATG |
NAG |
2 |
0.1691 |
Tier III |
| 12 |
NC_000001.11 |
1 |
46677880 |
+ |
EFCAB14-AS1 |
NNNTTGAGGGATACCTTATG |
NAG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
93585533 |
- |
Pola1 |
NNNTTGAGGGAAACCATATA |
NGG |
2 |
0.75 |
Tier II |
| 2 |
NC_000077.6 |
11 |
20827364 |
+ |
Lgalsl |
NNNTTAAGGGATGCCATATG |
NGG |
2 |
0.6522 |
Tier II |
| 3 |
NC_000074.6 |
8 |
16596685 |
+ |
Csmd1 |
NNNTGAAGGGATACCATATG |
NGG |
2 |
0.64 |
Tier II |
| 4 |
NC_000076.6 |
10 |
9421600 |
+ |
Samd5 |
NNNTAGAGGAATACCATATG |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000081.6 |
15 |
35983644 |
+ |
Rgs22 |
NNNTTGAAGGATACCATGTG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000078.6 |
12 |
36533518 |
- |
Crppa |
NNNTTGAGGGTTACCACATG |
NGG |
2 |
0.2172 |
Tier II |
| 7 |
NC_000086.7 |
X |
85582613 |
+ |
Tab3 |
NNNTTTAGGGATTCCATATG |
NGG |
2 |
0.2 |
Tier II |
| 8 |
NC_000067.6 |
1 |
74543395 |
+ |
Plcd4 |
NNNTTGAGGGTTACCAAATG |
NGG |
2 |
0.1641 |
Tier II |
| 9 |
NC_000067.6 |
1 |
74543395 |
+ |
Usp37 |
NNNTTGAGGGTTACCAAATG |
NGG |
2 |
0.1641 |
Tier II |
| 10 |
NC_000082.6 |
16 |
60576308 |
- |
Epha6 |
NNNTTGAGGGATTCCATATC |
NGG |
2 |
0.1286 |
Tier II |
| 11 |
NC_000072.6 |
6 |
29959490 |
+ |
Strip2 |
NNNTTGAGAGATAGCATATG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000076.6 |
10 |
72986260 |
+ |
A330049N07Rik |
NNNTTGGGGGATACCATATG |
NGA |
2 |
0.049 |
Tier III |
| 13 |
NC_000072.6 |
6 |
29959490 |
+ |
Gm52897 |
NNNTTGAGAGATAGCATATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
