Construct: sgRNA BRDN0001147171

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCGGATGATCAGATCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKP (5214)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77628

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 3113184 + PFKP NNNGGATGATCAGATCCCAA NGG 0 1.0 Tier I
2 NC_000023.11 X 65030785 - ZC4H2 NNNGAATGATCAGATACCAA NGG 2 0.8667 Tier II
3 NC_000002.12 2 53877640 + PSME4 NNNGGGTGATCAGATACCAA NGG 2 0.7143 Tier II
4 NC_000005.10 5 14820646 + ANKH NNNGGGTGATCAGATACCAA NGG 2 0.7143 Tier II
5 NC_000019.10 19 3467469 + NFIC NNNGGACAATCAGATCCCAA NGG 2 0.6875 Tier II
6 NC_000002.12 2 153817398 - GALNT13 NNNGGATGATCAGATATCAA NGG 2 0.4667 Tier II
7 NC_000011.10 11 41038325 + LRRC4C NNNGGGGGATCAGATCCCAA NGG 2 0.4202 Tier II
8 NC_000004.12 4 15530787 - CC2D2A NNNGGCTGATCAGATCCCAG NGG 2 0.3476 Tier II
9 NC_000007.14 7 150324989 - LRRC61 NNNGGATGAGCAGATCTCAA NGG 2 0.2333 Tier II
10 NC_000009.12 9 68424489 - PGM5 NNNGGGTGATTAGATCCCAA NGG 2 0.2198 Tier II
11 NC_000002.12 2 85170105 + TCF7L1 NNNGGAGGATGAGATCCCAA NGG 2 0.1471 Tier II
12 NC_000001.11 1 21521067 - ALPL NNNGGATGATCAGATTACAA NGG 2 0.1436 Tier II
13 NC_000005.10 5 133821916 + FSTL4 NNNGGAGGATCAGATGCCAA NGG 2 0.0905 Tier II
14 NC_000005.10 5 107474190 + EFNA5 NNNGGATGATCAGATGCCAC NGG 2 0.035 Tier II
15 NC_000011.10 11 8721194 + DENND2B NNNGGATTATCAGAGCCCAA NGG 2 0.0313 Tier II
16 NC_000011.10 11 36435621 + PRR5L NNNGGATGATTAGAGCCCAA NGG 2 0.0154 Tier II
17 NC_000009.12 9 13917196 + LOC101929507 NNNGAAAGATCAGATCCCAA NGG 2 0.7583 Tier III
18 NC_000014.9 14 44783609 + LINC02302 NNNGGATGATCAGATATCAA NGG 2 0.4667 Tier III
19 NC_000014.9 14 97776400 - LOC105370651 NNNGGATGATCAGATATCAA NGG 2 0.4667 Tier III
20 NC_000003.12 3 104842048 + LOC107986108 NNNGGATGATCAGATGCCAA NGG 1 0.1538 Tier III
21 NC_000012.12 12 15282662 - LOC105369673 NNNGGATGCTCAGATCCCAA NCG 2 0.0612 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 65380030 + Klb NNNGGATGATCAGATCCGAA NGT 2 0.0022 Tier I
2 NC_000071.6 5 92656901 + Ccdc158 NNNGGGTGAGCAGATCCCAA NGG 2 0.3571 Tier II
3 NC_000068.7 2 106402818 + Dcdc5 NNNGGATGTTCAGATCACAA NGG 2 0.28 Tier II
4 NC_000081.6 15 65877041 + Oc90 NNNGGATGGTTAGATCCCAA NGG 2 0.2051 Tier II
5 NC_000072.6 6 116633637 - Rassf4 NNNGGATCATCAGACCCCAA NGG 2 0.1678 Tier II
6 NC_000069.6 3 103638156 - Syt6 NNNGGATGCTCAGATCCCAA NAG 2 0.1481 Tier II
7 NC_000079.6 13 30808209 - Exoc2 NNNGGCTGATCAGATTCCAA NGG 2 0.1399 Tier II
8 NC_000077.6 11 70542501 + Pld2 NNNGGATCATCAGATCCCCA NGG 2 0.1273 Tier II
9 NC_000069.6 3 76263928 + Fstl5 NNNGGATGATCACATGCCAA NGG 2 0.0648 Tier II
10 NC_000076.6 10 90034925 + Anks1b NNNGGATGATCAGATTCCAA NGC 2 0.0068 Tier II
11 NC_000070.6 4 47635681 - Gm32544 NNNGGATGAGCAGATACCAA NGG 2 0.5 Tier III
12 NC_000082.6 16 12809591 - 4930414F18Rik NNNGCATCATCAGATCCCAA NGG 2 0.4835 Tier III
13 NC_000072.6 6 52248894 + Hoxa11os NNNGGATGCTCAGATCCCTA NGG 2 0.3077 Tier III
14 NC_000068.7 2 106402818 + Gm46755 NNNGGATGTTCAGATCACAA NGG 2 0.28 Tier III
15 NC_000077.6 11 54822020 + Lyrm7os NNNGGATGAGCAGAGCCCAA NGG 2 0.025 Tier III
16 NC_000068.7 2 52384372 + Gm13522 NNNGGAAGATCAGATCCCAA NGC 2 0.0194 Tier III
17 NC_000078.6 12 8148267 - Gm33037 NNNGGATGATCAGAGCCCAA NAG 2 0.013 Tier III
Download CSV

Other clones with same target sequence:

(none)