Construct: sgRNA BRDN0001147172

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGATCATCCAGTTCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYLK (4638)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77021

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 123666320 - MYLK NNNGATCATCCAGTTCCCTG NGG 0 1.0 Tier I
2 NC_000020.11 20 37229995 + RPN2 NNNGGTCATCAAGTTCCCTG NGG 2 0.54 Tier I
3 NC_000009.12 9 116865672 + ASTN2 NNNGATAAACCAGTTCCCTG NGG 2 0.6964 Tier II
4 NC_000009.12 9 91334375 + AUH NNNCATCATCCAATTCCCTG NGG 2 0.4887 Tier II
5 NC_000019.10 19 56870238 + LOC124904779 NNNAATCTTCCAGTTCCCTG NGG 2 0.3857 Tier II
6 NC_000022.11 22 32612445 - SYN3 NNNGATTATCCAGTTCTCTG NGG 2 0.3792 Tier II
7 NC_000018.10 18 63126202 + BCL2 NNNGATCATTCTGTTCCCTG NGG 2 0.3137 Tier II
8 NC_000005.10 5 65587012 + PPWD1 NNNGATCTTCCAGTTCCCTT NGG 2 0.3 Tier II
9 NC_000001.11 1 10775342 + CASZ1 NNNCATCATCCAGTTCCCTC NGG 2 0.2269 Tier II
10 NC_000002.12 2 205287878 + PARD3B NNNGATCATAGAGTTCCCTG NGG 2 0.2167 Tier II
11 NC_000015.10 15 49893043 - ATP8B4 NNNCTTCATCCAGTTCCCTG NGG 2 0.1925 Tier II
12 NC_000022.11 22 26490318 - SRRD NNNCTTCATCCAGTTCCCTG NGG 2 0.1925 Tier II
13 NC_000011.10 11 125264310 - PKNOX2 NNNCATCATCCAGTTTCCTG NGG 2 0.1629 Tier II
14 NC_000007.14 7 30653850 + CRHR2 NNNGATCATCCAGTTTCCTC NGG 2 0.1319 Tier II
15 NC_000003.12 3 14566274 - GRIP2 NNNTATCATCCAGTCCCCTG NGG 2 0.0992 Tier II
16 NC_000021.9 21 32460645 + EVA1C NNNGATCATCCAGTCCCGTG NGG 2 0.0364 Tier II
17 NC_000014.9 14 93531041 + UNC79 NNNGATCATCCAGTGCTCTG NGG 2 0.0233 Tier II
18 NC_000011.10 11 12134135 + MICAL2 NNNGATCATCCAGTTCCCAG NGT 2 0.0046 Tier II
19 NC_000012.12 12 101287984 + UTP20 NNNGATCATCCAGGGCCCTG NGG 2 0.0 Tier II
20 NC_000009.12 9 33380875 - LOC124902142 NNNGAACAGCCAGTTCCCTG NGG 2 0.5365 Tier III
21 NC_000016.10 16 49112979 + LOC105371241 NNNGAACAGCCAGTTCCCTG NGG 2 0.5365 Tier III
22 NC_000010.11 10 65827711 + LOC105378339 NNNGATCTGCCAGTTCCCTG NGG 2 0.2653 Tier III
23 NC_000011.10 11 125264310 - PKNOX2-AS1 NNNCATCATCCAGTTTCCTG NGG 2 0.1629 Tier III
24 NC_000007.14 7 30653850 + LOC124901609 NNNGATCATCCAGTTTCCTC NGG 2 0.1319 Tier III
25 NC_000009.12 9 99785104 + LOC101928438 NNNGATCCTCCAGTTCCCTG NAG 2 0.1111 Tier III
26 NC_000002.12 2 103957589 + LINC01965 NNNGATCACCCAGTTCCCTG NGC 2 0.0205 Tier III
27 NC_000007.14 7 36032739 + LOC105375233 NNNCATCATCCAGGTCCCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 34953606 + Mylk NNNAATCATCCAGTTCCCCG NGG 2 0.2483 Tier I
2 NC_000074.6 8 122780176 - Acsf3 NNNGATCATACAGTTCCCTG NAG 2 0.2247 Tier I
3 NC_000080.6 14 39073199 - Nrg3 NNNGATCATTCAGTTCCCTG NGG 1 0.9412 Tier II
4 NC_000070.6 4 117298702 - Rnf220 NNNGAGCATCCAGTTCCCTG NGG 1 0.5714 Tier II
5 NC_000079.6 13 68845981 + Adcy2 NNNGATCATACAGTTCCTTG NGG 2 0.5571 Tier II
6 NC_000067.6 1 126412921 + Nckap5 NNNGATAATCCAGTTCCTTG NGG 2 0.4821 Tier II
7 NC_000068.7 2 105941741 + Immp1l NNNGATCATCAAGTTCCTTG NGG 2 0.4821 Tier II
8 NC_000068.7 2 24342874 - Il1rn NNNGACCATCCAGTTCTCTG NGG 2 0.4242 Tier II
9 NC_000073.6 7 118571544 + Tmc7 NNNGGGCATCCAGTTCCCTG NGG 2 0.4114 Tier II
10 NC_000077.6 11 3141704 - Sfi1 NNNGACCATGCAGTTCCCTG NGG 2 0.3535 Tier II
11 NC_000084.6 18 64969973 - Nedd4l NNNGATCACCCAGTTCCCAG NGG 2 0.2637 Tier II
12 NC_000070.6 4 104979810 + Fyb2 NNNAATCATCCAGTTCCCTG NAG 2 0.2333 Tier II
13 NC_000083.6 17 64079688 + Fer NNNGAGCATCCAGTCCCCTG NGG 2 0.1558 Tier II
14 NC_000082.6 16 34520475 + Kalrn NNNGATCATGGAGTTCCCTG NGG 2 0.0972 Tier II
15 NC_000067.6 1 75049319 - Nhej1 NNNGATCATGCAGTTGCCTG NGG 2 0.0598 Tier II
16 NC_000072.6 6 29713569 + Tspan33 NNNGATCACCCAGTGCCCTG NGG 2 0.0462 Tier II
17 NC_000080.6 14 22118302 + Lrmda NNNGAGCATCCAGTTCCCTG NGA 2 0.0397 Tier II
18 NC_000069.6 3 89350943 - Dcst1 NNNGATCATCCAGTTCCCTG NAA 2 0.0 Tier II
19 NC_000069.6 3 89350943 - Dcst2 NNNGATCATCCAGTTCCCTG NAA 2 0.0 Tier II
20 NC_000078.6 12 3843343 + Dnmt3a NNNGATCCTCCAGGTCCCTG NGG 2 0.0 Tier II
21 NC_000070.6 4 117298702 - Gm12843 NNNGAGCATCCAGTTCCCTG NGG 1 0.5714 Tier III
22 NC_000072.6 6 54003448 + 4921529L05Rik NNNAAGCATCCAGTTCCCTG NGG 2 0.5143 Tier III
23 NC_000068.7 2 50548023 - Gm13485 NNNGAGCATCAAGTTCCCTG NGG 2 0.4286 Tier III
24 NT_187064.1 42682 + LOC100861749 NNNGACCATGCAGTTCCCTG NGG 2 0.3535 Tier III
25 NT_187064.1 42682 + LOC105247294 NNNGACCATGCAGTTCCCTG NGG 2 0.3535 Tier III
26 NC_000073.6 7 68809945 + Gm34612 NNNGATCCTCCAGTTCCCTT NGG 2 0.3 Tier III
27 NC_000082.6 16 34953606 + E130310I04Rik NNNAATCATCCAGTTCCCCG NGG 2 0.2483 Tier III
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Other clones with same target sequence:

(none)