Construct: sgRNA BRDN0001147180

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGCCAACTCAGGTAAACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK17B (9262)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76799

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 196146026 + STK17B NNNCCAACTCAGGTAAACAC NGG 0 1.0 Tier I
2 NC_000012.12 12 40528313 + MUC19 NNNCCAACTCAGGTAAGCAC NGA 2 0.0123 Tier I
3 NC_000011.10 11 83248050 + ANKRD42 NNNTCAACTCAGGGAAACAC NGG 2 0.0 Tier I
4 NC_000004.12 4 75980668 + SDAD1 NNNCCAACACAGGAAAACAC NGG 2 0.5748 Tier II
5 NC_000015.10 15 81103677 - CFAP161 NNNAGAACTCAGGTAAACAC NGG 2 0.5053 Tier II
6 NC_000001.11 1 64953481 + JAK1 NNNCCCACACAGGTAAACAC NGG 2 0.4221 Tier II
7 NC_000014.9 14 50631821 + ATL1 NNNCCCACTCAGGAAAACAC NGG 2 0.2814 Tier II
8 NC_000006.12 6 45534671 + RUNX2 NNNCCCTCTCAGGTAAACAC NGG 2 0.1989 Tier II
9 NC_000001.11 1 245696130 - KIF26B NNNCCAACTCTGGTAAATAC NGG 2 0.1978 Tier II
10 NC_000015.10 15 38548877 + RASGRP1 NNNCCATCTCAGGTAGACAC NGG 2 0.0841 Tier II
11 NC_000003.12 3 159470970 - SCHIP1 NNNCCAACTCAGGCAACCAC NGG 2 0.0504 Tier II
12 NC_000003.12 3 159470970 - IQCJ-SCHIP1 NNNCCAACTCAGGCAACCAC NGG 2 0.0504 Tier II
13 NC_000010.11 10 5648943 + ASB13 NNNCCCACTCAGGTAAACAC NCG 2 0.0487 Tier II
14 NC_000007.14 7 44963960 + MYO1G NNNCCATCTCAGGTAAACAG NGG 2 0.0257 Tier II
15 NC_000020.11 20 63533992 + PTK6 NNNCCAACTCAGGCAAACAC NTG 2 0.0111 Tier II
16 NC_000001.11 1 245696130 - LOC105373265 NNNCCAACTCTGGTAAATAC NGG 2 0.1978 Tier III
17 NC_000009.12 9 119434510 + LOC105376250 NNNCCAACTCTGGTAAACTC NGG 2 0.1657 Tier III
18 NC_000021.9 21 7637929 + CTBP2P9 NNNCCAACTCAGGAAACCAC NGG 2 0.1092 Tier III
19 NC_000007.14 7 8275954 + ICA1-AS1 NNNCCAAATCAGGTAAACAC NTG 2 0.0253 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 53766036 + Stk17b NNNCTAACTCAGGTAAACAC NGG 1 0.6364 Tier I
2 NC_000083.6 17 21314411 - Vmn1r237 NNNCCAACTCATGTACACAC NGG 2 0.0 Tier I
3 NC_000085.6 19 5117507 + Klc2 NNNCCAAGTTAGGTAAACAC NGG 2 0.605 Tier II
4 NC_000081.6 15 35977408 - Rgs22 NNNCCAACTCAAGAAAACAC NGG 2 0.5778 Tier II
5 NC_000074.6 8 78648512 - Slc10a7 NNNCGAACTCAGATAAACAC NGG 2 0.5538 Tier II
6 NC_000079.6 13 64862837 + Cntnap3 NNNCCAAAGCAGGTAAACAC NGG 2 0.4024 Tier II
7 NC_000074.6 8 75759832 - Iqcm NNNCCAACTGAGGTAAACAC NGG 1 0.3889 Tier II
8 NC_000076.6 10 70131331 - Ccdc6 NNNCCAACCCAGGCAAACAC NGG 2 0.2637 Tier II
9 NC_000076.6 10 84925783 - Ric8b NNNCCAACTCATGTAAGCAC NGG 2 0.0679 Tier II
10 NC_000072.6 6 12592663 + Thsd7a NNNCCAACTCAGGCAAACCC NGG 2 0.0591 Tier II
11 NC_000080.6 14 117532237 + Gpc6 NNNCCAACTCAGGTAAAGAC NGA 2 0.0093 Tier II
12 NC_000075.6 9 64455131 - Megf11 NNNCCAACACAGGGAAACAC NGG 2 0.0 Tier II
13 NC_000074.6 8 65035514 - Apela NNNCCAACTCAGCTACACAC NGG 2 0.0 Tier II
14 NC_000086.7 X 155615855 - Ptchd1 NNNCCAACTCAGGGAGACAC NGG 2 0.0 Tier II
15 NC_000075.6 9 109601669 - Fbxw24 NNNCCCACTCAGGTACACAC NGG 2 0.0 Tier II
16 NC_000085.6 19 5117507 + LOC115489018 NNNCCAAGTTAGGTAAACAC NGG 2 0.605 Tier III
17 NC_000084.6 18 38172090 - Gm38566 NNNACAGCTCAGGTAAACAC NGG 2 0.5944 Tier III
18 NC_000080.6 14 103721529 + Gm35197 NNNCCAACTTAGGAAAACAC NGG 2 0.5826 Tier III
19 NC_000079.6 13 44635111 + Gm40931 NNNCCAACTAAGGTAAACAC NGC 2 0.0193 Tier III
20 NC_000081.6 15 102760931 + Gm36560 NNNCCAACTCAGGTAAACAA NGC 2 0.0111 Tier III
Download CSV

Other clones with same target sequence:

(none)