Construct: sgRNA BRDN0001147183
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAGGGAGCGGCAACACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- UCKL1 (54963)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76347
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63944447 |
- |
UCKL1 |
NNNAGGGAGCGGCAACACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
34942305 |
+ |
NBEA |
NNNAGGGGGCGGCAACATGG |
NGG |
2 |
0.4714 |
Tier II |
| 3 |
NC_000012.12 |
12 |
111799869 |
- |
ALDH2 |
NNNAGGGTTCGGCAACACGG |
NGG |
2 |
0.2286 |
Tier II |
| 4 |
NC_000002.12 |
2 |
1864392 |
- |
MYT1L |
NNNAGGGAGCGGCAAGACTG |
NGG |
2 |
0.1026 |
Tier II |
| 5 |
NC_000006.12 |
6 |
5762869 |
+ |
FARS2 |
NNNAGGGAGCGGCAACACTG |
NTG |
2 |
0.026 |
Tier II |
| 6 |
NC_000012.12 |
12 |
111799869 |
- |
MIR6761 |
NNNAGGGTTCGGCAACACGG |
NGG |
2 |
0.2286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
141870733 |
- |
Sdk1 |
NNNAGGGAGAGGCAACATGG |
NGG |
2 |
0.5571 |
Tier II |
| 2 |
NC_000069.6 |
3 |
56183716 |
- |
Nbea |
NNNAGGGGGCGGCAACATGG |
NGG |
2 |
0.4714 |
Tier II |
| 3 |
NC_000070.6 |
4 |
47112566 |
- |
Galnt12 |
NNNAGGGAGGGGCAACACGG |
NGG |
1 |
0.3889 |
Tier II |
| 4 |
NC_000075.6 |
9 |
62851405 |
+ |
Cln6 |
NNNAGGGAGCGGCAGCACGG |
NAG |
2 |
0.1685 |
Tier II |
| 5 |
NC_000067.6 |
1 |
118852084 |
- |
Gli2 |
NNNAGGGAGGGGCACCACGG |
NGG |
2 |
0.1061 |
Tier II |
| 6 |
NC_000081.6 |
15 |
84232242 |
- |
Parvb |
NNNAGGGAGCGGACACACGG |
NGG |
2 |
0.0824 |
Tier II |
| 7 |
NC_000069.6 |
3 |
135626559 |
- |
Nfkb1 |
NNNAGGGAGCGGGAACACGC |
NGG |
2 |
0.0584 |
Tier II |
| 8 |
NC_000070.6 |
4 |
155219753 |
+ |
Ski |
NNNAGGGAGCAGCAACACGG |
NTG |
2 |
0.039 |
Tier II |
| 9 |
NC_000075.6 |
9 |
62851405 |
+ |
Gm10653 |
NNNAGGGAGCGGCAGCACGG |
NAG |
2 |
0.1685 |
Tier III |
| 10 |
NC_000084.6 |
18 |
43847123 |
+ |
Gm41715 |
NNNAGGGATCGGCACCACGG |
NGG |
2 |
0.1455 |
Tier III |
Other clones with same target sequence:
(none)
