Construct: sgRNA BRDN0001147184

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTCACAGAACATCAACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK3 (23678)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75542

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 66828680 + SGK3 NNNTCACAGAACATCAACCT NGG 0 1.0 Tier I
2 NC_000008.11 8 66828680 + C8orf44-SGK3 NNNTCACAGAACATCAACCT NGG 0 1.0 Tier I
3 NC_000009.12 9 74583630 - RORB NNNTGACAGAACATCAACCT NGG 1 0.6 Tier II
4 NC_000012.12 12 40814938 - CNTN1 NNNTCAAAGAAAATCAACCT NGG 2 0.5357 Tier II
5 NC_000010.11 10 115935178 - ATRNL1 NNNTCAAAGAACATCAAACT NGG 2 0.4038 Tier II
6 NC_000022.11 22 42916395 + PACSIN2 NNNTCACAGAGCAACAACCT NGG 2 0.4024 Tier II
7 NC_000013.11 13 29518970 + SLC7A1 NNNTCTCTGAACATCAACCT NGG 2 0.3061 Tier II
8 NC_000014.9 14 73310546 + NUMB NNNTGACAGAACATCAACAT NGG 2 0.2571 Tier II
9 NC_000008.11 8 39756833 + ADAM2 NNNTCATAGAACATCAACCT NAG 2 0.2106 Tier II
10 NC_000013.11 13 35136366 - NBEA NNNTCACAGAACATCAATCT NAG 2 0.1667 Tier II
11 NC_000007.14 7 103769752 + RELN NNNTCACAGAACATCAACTT NAG 2 0.1197 Tier II
12 NC_000001.11 1 176114068 - COP1 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier II
13 NC_000003.12 3 131881085 + CPNE4 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier II
14 NC_000004.12 4 167177811 - SPOCK3 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier II
15 NC_000007.14 7 103834439 - RELN NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier II
16 NC_000023.11 X 106697395 - RNF128 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier II
17 NC_000020.11 20 15407833 + MACROD2 NNNTCACAGAACATCAGACT NGG 2 0.095 Tier II
18 NC_000020.11 20 31698287 - BCL2L1 NNNTCACAGAACTCCAACCT NGG 2 0.0857 Tier II
19 NC_000011.10 11 126780588 + KIRREL3 NNNTGACAGAACATCAAGCT NGG 2 0.08 Tier II
20 NC_000001.11 1 214480659 + PTPN14 NNNTCACAGCACCTCAACCT NGG 2 0.0702 Tier II
21 NC_000001.11 1 201788757 + NAV1 NNNTCACAGAACATCAAGTT NGG 2 0.0615 Tier II
22 NC_000007.14 7 31853055 + PDE1C NNNTCACAGAACATCAGCCC NGG 2 0.016 Tier II
23 NC_000011.10 11 132223525 + NTM NNNTCACAGAACATCAACAT NGC 2 0.0095 Tier II
24 NC_000007.14 7 20188261 + MACC1 NNNTCACAGAACTTCAACCT NGT 2 0.0048 Tier II
25 NC_000023.11 X 67687444 - AR NNNTCACAGAACATCTACCT NTG 2 0.0 Tier II
26 NC_000008.11 8 6540938 + ANGPT2 NNNTCTCAGAACATCCACCT NGG 2 0.0 Tier II
27 NC_000008.11 8 6540938 + MCPH1 NNNTCTCAGAACATCCACCT NGG 2 0.0 Tier II
28 NC_000003.12 3 44045249 - LOC124909489 NNNGCAGAGAACATCAACCT NGG 2 0.2941 Tier III
29 NC_000013.11 13 35136366 - LOC124903232 NNNTCACAGAACATCAATCT NAG 2 0.1667 Tier III
30 NC_000003.12 3 184416293 - LINC02054 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier III
31 NC_000004.12 4 157996435 - LOC105377509 NNNTCACAGAACATCAACAT NAG 2 0.1111 Tier III
32 NC_000017.11 17 70062232 - LINC01028 NNNTCACAGCACTTCAACCT NGG 2 0.1 Tier III
33 NC_000017.11 17 70062232 - LOC105371881 NNNTCACAGCACTTCAACCT NGG 2 0.1 Tier III
34 NC_000020.11 20 31698287 - BCL2L1-AS1 NNNTCACAGAACTCCAACCT NGG 2 0.0857 Tier III
35 NC_000012.12 12 93187859 + LOC643339 NNNTCACAGAACACCAACCT NAG 2 0.0741 Tier III
36 NC_000001.11 1 201788757 + IPO9-AS1 NNNTCACAGAACATCAAGTT NGG 2 0.0615 Tier III
37 NC_000018.10 18 47376789 - MIR4527HG NNNTCACAGAACCTCAAGCT NGG 2 0.0281 Tier III
38 NC_000002.12 2 81786195 - LOC102724542 NNNTCACAGAACATTAACCT NAG 2 0.0173 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 9879052 + Sgk3 NNNTCACGGAACATCAACCT NGG 1 0.7333 Tier I
2 NC_000068.7 2 180055039 + Ss18l1 NNNACACAGAACATGAACCT NGG 2 0.0238 Tier I
3 NC_000079.6 13 17622198 + Sugct NNNTCACAGAATATCAATCT NGG 2 0.3462 Tier II
4 NC_000068.7 2 118908100 + Bahd1 NNNACTCAGAACATCAACCT NGG 2 0.3401 Tier II
5 NC_000070.6 4 107597903 - Glis1 NNNACACAGAACAACAACCT NGG 2 0.2948 Tier II
6 NC_000067.6 1 135465716 - Nav1 NNNTCACAGGACATCAACTT NGG 2 0.2564 Tier II
7 NC_000073.6 7 115980013 - Sox6 NNNTCAGAGAACATCAACTT NGG 2 0.2172 Tier II
8 NC_000071.6 5 143037102 + Rnf216 NNNTCTCAGAACTTCAACCT NGG 2 0.2143 Tier II
9 NC_000073.6 7 134805683 - Dock1 NNNTCACAGATCATCAATCT NGG 2 0.1978 Tier II
10 NC_000071.6 5 48479768 - Kcnip4 NNNTCATAGAACATCAGCCT NGG 2 0.1434 Tier II
11 NC_000080.6 14 75249349 - Cpb2 NNNTCATAGAACATCAGCCT NGG 2 0.1434 Tier II
12 NC_000072.6 6 77222495 - Ctnna2 NNNTCACAGAGCATCACCCT NGG 2 0.1147 Tier II
13 NC_000074.6 8 114966403 + Wwox NNNTAACAGAACATCAACCT NGA 2 0.0397 Tier II
14 NC_000069.6 3 103152266 - Dennd2c NNNGCACAGAACATCAACCT NTG 2 0.0244 Tier II
15 NC_000082.6 16 18920075 - Hira NNNTCACAGAACATCAGCCT NTG 2 0.0069 Tier II
16 NC_000071.6 5 87554928 - Sult1d1 NNNTCACAGAACAGCAACCT NGG 1 0.0 Tier II
17 NC_000067.6 1 152674546 + Rgl1 NNNTCACAGAAGAGCAACCT NGG 2 0.0 Tier II
18 NC_000078.6 12 91556057 + Gtf2a1 NNNTCACAGAAGAGCAACCT NGG 2 0.0 Tier II
19 NC_000083.6 17 74042598 + Srd5a2 NNNTCACAGAATATCCACCT NGG 2 0.0 Tier II
20 NC_000081.6 15 79213308 - Gm10863 NNNCCACAGGACATCAACCT NGG 2 0.3595 Tier III
21 NC_000071.6 5 29690890 + Gm42245 NNNACACAGGACATCAACCT NGG 2 0.2646 Tier III
22 NC_000082.6 16 91171297 + Gm9881 NNNTCACAGAAAATAAACCT NGG 2 0.1587 Tier III
23 NC_000082.6 16 91171297 + Gm41492 NNNTCACAGAAAATAAACCT NGG 2 0.1587 Tier III
24 NC_000077.6 11 26208744 - 5730522E02Rik NNNTCAGAGATCATCAACCT NGG 2 0.1448 Tier III
25 NC_000078.6 12 109501200 + Gm34081 NNNTCACTGAACATTAACCT NGG 2 0.0286 Tier III
26 NC_000070.6 4 101378455 + 0610043K17Rik NNNTCACATAACAGCAACCT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)