Construct: sgRNA BRDN0001147185

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGTAGTAAATATCTAGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
Negative control (unspec'd)
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80236

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 32867208 - DMD NNNAGTAAAAATCTAGCAAA NGG 2 0.5714 Tier II
2 NC_000007.14 7 117820355 - CTTNBP2 NNNAGTAAAGATCTAACTAA NGG 2 0.5 Tier II
3 NC_000004.12 4 168894057 - PALLD NNNAGTAAATGTTTAGCTAA NGG 2 0.455 Tier II
4 NC_000016.10 16 84135473 + HSDL1 NNNAGTAAATGTCTAGCAAA NGG 2 0.4333 Tier II
5 NC_000021.9 21 31524742 - TIAM1 NNNTGTAATTATCTAGCTAA NGG 2 0.3818 Tier II
6 NC_000001.11 1 210169678 - SYT14 NNNAGTAAAAATCTAGCCAA NGG 2 0.3673 Tier II
7 NC_000003.12 3 53003939 - SFMBT1 NNNTGGAAATATCTAGCTAA NGG 2 0.3636 Tier II
8 NC_000005.10 5 151905262 - GLRA1 NNNAGTAGATATCTAGATAA NGG 2 0.3422 Tier II
9 NC_000001.11 1 111782533 - KCND3 NNNTGTAAATATCTAGCCAA NGG 2 0.2727 Tier II
10 NC_000007.14 7 40776949 + SUGCT NNNAGTAAATATCAAGCCAA NGG 2 0.2653 Tier II
11 NC_000018.10 18 37443827 - CELF4 NNNAGTAAATATCTGGCGAA NGG 2 0.2167 Tier II
12 NC_000007.14 7 24665247 + PALS2 NNNAGTAAATATCTTACTAA NGG 2 0.2 Tier II
13 NC_000012.12 12 32845174 - PKP2 NNNAGTAAATTTCTGGCTAA NGG 2 0.2 Tier II
14 NC_000016.10 16 71535105 - CHST4 NNNAGTAAATATCTAGAGAA NGG 2 0.1556 Tier II
15 NC_000009.12 9 94812217 - AOPEP NNNAGTAAATATCTAGCTCT NGG 2 0.1241 Tier II
16 NC_000012.12 12 28437638 + CCDC91 NNNAATAAATATCTAGCTAA NGA 2 0.0602 Tier II
17 NC_000004.12 4 61625553 - ADGRL3 NNNAGAAAATATCTAGGTAA NGG 2 0.051 Tier II
18 NC_000004.12 4 105261570 - TET2 NNNAGTAAATATCTAGCTAT NTG 2 0.0234 Tier II
19 NC_000003.12 3 197068943 - DLG1 NNNAGTAAATATCTAGTTAA NTG 2 0.0182 Tier II
20 NC_000008.11 8 42723167 - CHRNB3 NNNAGTAAATATCTAGGTAA NAG 2 0.0153 Tier II
21 NC_000011.10 11 85013638 + DLG2 NNNAGTAAATATCTAGCTCA NGA 2 0.0144 Tier II
22 NC_000015.10 15 44608797 - SPG11 NNNATTAAATATCTAGCTAA NGT 2 0.0048 Tier II
23 NC_000007.14 7 116954644 + ST7 NNNAGTAAATTTCGAGCTAA NGG 2 0.0 Tier II
24 NC_000010.11 10 2472465 - LINC02645 NNNAGTAAATATCTAACTAA NGG 1 1.0 Tier III
25 NC_000001.11 1 96298982 - LINC01787 NNNAGAAAATATCTAGCTAA NGG 1 0.8667 Tier III
26 NC_000002.12 2 181174042 + LINC01934 NNNGGTAAATATCTAGCAAA NGG 2 0.4167 Tier III
27 NC_000004.12 4 171282242 + LOC105377534 NNNAGTAAATAAATAGCTAA NGG 2 0.3077 Tier III
28 NC_000018.10 18 64162320 - LINC01924 NNNAGTAAGTTTCTAGCTAA NGG 2 0.2051 Tier III
29 NC_000001.11 1 84637097 + LOC124904206 NNNAGTAAATCTCTAGCCAA NGG 2 0.1714 Tier III
30 NC_000018.10 18 35463840 - ZNF24TR NNNAGTAAATAACTAGCTAA NGA 2 0.0556 Tier III
31 NC_000004.12 4 105261570 - TET2-AS1 NNNAGTAAATATCTAGCTAT NTG 2 0.0234 Tier III
32 NC_000002.12 2 67211082 - LINC01829 NNNAGTAAATTTCTAGCTAA NGA 2 0.0214 Tier III
33 NC_000002.12 2 67211082 - LINC01828 NNNAGTAAATTTCTAGCTAA NGA 2 0.0214 Tier III
34 NC_000007.14 7 116954644 + ST7-OT4 NNNAGTAAATTTCGAGCTAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 93042012 + Ces1a NNNAGTAAATATTTAGGTAA NGG 2 0.0412 Tier I
2 NC_000079.6 13 112626749 - Ddx4 NNNAGTAGAAATCTAGCTAA NGG 2 0.6286 Tier II
3 NC_000070.6 4 84543497 - Bnc2 NNNAGTAAAAATTTAGCTAA NGG 2 0.6 Tier II
4 NC_000074.6 8 108878068 - Zfhx3 NNNAGAAAATATCTGGCTAA NGG 2 0.5633 Tier II
5 NC_000086.7 X 87052456 + Il1rapl1 NNNAGTTAATATCTAACTAA NGG 2 0.4375 Tier II
6 NC_000068.7 2 12985488 + Pter NNNAGTAAATGTCTAGCAAA NGG 2 0.4333 Tier II
7 NC_000080.6 14 124440533 + Fgf14 NNNAGTAATTATCTAGCTAT NGG 2 0.36 Tier II
8 NC_000075.6 9 10141517 - Cntn5 NNNAATAAATATCTAGCTAA NAG 2 0.2247 Tier II
9 NC_000076.6 10 100089636 - Kitl NNNATTAAATATCTAGCAAA NGG 2 0.2 Tier II
10 NC_000071.6 5 77038244 - Thegl NNNAGTAAATATCCAGCAAA NGG 2 0.1905 Tier II
11 NC_000067.6 1 92083226 + Hdac4 NNNAGTAAATATCTAGTTCA NGG 2 0.0966 Tier II
12 NC_000070.6 4 149250521 - Kif1b NNNAGCAAATATCTAGCTAA NTG 2 0.0354 Tier II
13 NC_000071.6 5 5378613 + Cdk14 NNNAGTAACTATCTAGCTAA NGT 2 0.0092 Tier II
14 NC_000078.6 12 85916537 - Ttll5 NNNAGTAAATATCTAGCTAA NTA 2 0.0 Tier II
15 NC_000077.6 11 16786364 + Egfr NNNAGTAAATATCTATCTAT NGG 2 0.0 Tier II
16 NC_000079.6 13 71079552 + Gm40997 NNNAGCAAATACCTAGCTAA NGG 2 0.8612 Tier III
17 NC_000078.6 12 12771922 + Gm35104 NNNAGCAAATATTTAGCTAA NGG 2 0.6364 Tier III
18 NC_000069.6 3 112052491 - Gm6602 NNNTGAAAATATCTAGCTAA NGG 2 0.5515 Tier III
19 NC_000082.6 16 86855715 - Gm32624 NNNAGTAATTATCTGGCTAA NGG 2 0.39 Tier III
20 NC_000076.6 10 78594840 - Gm30346 NNNAGGAAATATCTGGCTAA NGG 2 0.3714 Tier III
21 NC_000072.6 6 60442646 - Gm38823 NNNATTAAATATCTAGCTAA NGG 1 0.3 Tier III
22 NC_000072.6 6 135559746 - Gm25136 NNNAGTAAATATCTAGCTAG NAG 2 0.1983 Tier III
23 NC_000086.7 X 69959207 + 1700111N16Rik NNNAGTAAATATCTAGATAA NTG 2 0.0182 Tier III
24 NC_000077.6 11 16786364 + Gm39584 NNNAGTAAATATCTATCTAT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)