Construct: sgRNA BRDN0001147186
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGAGGCGATACTTGGAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB3 (2065)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77479
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
56095797 |
+ |
ERBB3 |
NNNAGGCGATACTTGGAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
71865205 |
+ |
CALN1 |
NNNAGGAGATACTTGGATCG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000019.10 |
19 |
1776094 |
- |
ONECUT3 |
NNNAGGCGATTCTTGGCACG |
NGG |
2 |
0.0543 |
Tier II |
| 4 |
NC_000002.12 |
2 |
37325192 |
+ |
PRKD3-DT |
NNNAGGTGATAGTTGGAACG |
NGG |
2 |
0.3611 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
33588825 |
+ |
Lipo3 |
NNNAGGGGAAACTTGGAACG |
NGG |
2 |
0.4034 |
Tier II |
| 2 |
NC_000085.6 |
19 |
33760514 |
+ |
Lipo2 |
NNNAGGGGAAACTTGGAACG |
NGG |
2 |
0.4034 |
Tier II |
| 3 |
NC_000082.6 |
16 |
8727220 |
+ |
Usp7 |
NNNAGGAGATACTTGGAAAG |
NGG |
2 |
0.3214 |
Tier II |
| 4 |
NC_000083.6 |
17 |
50601841 |
- |
Plcl2 |
NNNAGGGGATACTTGGAAAG |
NGG |
2 |
0.2017 |
Tier II |
| 5 |
NC_000069.6 |
3 |
25636946 |
+ |
Nlgn1 |
NNNAGGCCATACTTTGAACG |
NGG |
2 |
0.0879 |
Tier II |
| 6 |
NC_000067.6 |
1 |
110109488 |
- |
Cdh7 |
NNNAGGCGATACTGGGAAAG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
