Construct: sgRNA BRDN0001147190
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGCAAGCATGATCTTTCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK2A2 (1459)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75587
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
58167249 |
- |
CSNK2A2 |
NNNCAAGCATGATCTTTCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
54356385 |
+ |
CIMIP6 |
NNNCAAGCATGATCTTCCCA |
NGG |
2 |
0.3164 |
Tier II |
| 3 |
NC_000012.12 |
12 |
106602640 |
+ |
RFX4 |
NNNCAAGCATGATCTTTCCT |
NGG |
2 |
0.269 |
Tier II |
| 4 |
NC_000022.11 |
22 |
41301385 |
+ |
RANGAP1 |
NNNCAGGCATGAGCTTTCGA |
NGG |
2 |
0.1863 |
Tier II |
| 5 |
NC_000006.12 |
6 |
134272990 |
- |
SGK1 |
NNNCAAGCATCCTCTTTCGA |
NGG |
2 |
0.1128 |
Tier II |
| 6 |
NC_000007.14 |
7 |
93440410 |
- |
CALCR |
NNNCAAGCATTATCTTTGGA |
NGG |
2 |
0.0513 |
Tier II |
| 7 |
NC_000012.12 |
12 |
106602640 |
+ |
LOC100287944 |
NNNCAAGCATGATCTTTCCT |
NGG |
2 |
0.269 |
Tier III |
| 8 |
NC_000010.11 |
10 |
78312440 |
- |
LOC124902468 |
NNNCAAGCATGTTCTTTAGA |
NGG |
2 |
0.1795 |
Tier III |
| 9 |
NC_000021.9 |
21 |
25416158 |
+ |
LINC00158 |
NNNCAAGCATGATCTTGAGA |
NGG |
2 |
0.0633 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
134227779 |
+ |
Adora1 |
NNNCAAGCTTGATCTATCGA |
NGG |
2 |
0.5455 |
Tier II |
| 2 |
NC_000076.6 |
10 |
80140866 |
+ |
Atp5d |
NNNCAAGCATGATCTTTCTA |
NCG |
2 |
0.0714 |
Tier II |
| 3 |
NC_000085.6 |
19 |
38195773 |
+ |
Fra10ac1 |
NNNCAAGCATCATCTTTGGA |
NGG |
2 |
0.0571 |
Tier II |
| 4 |
NC_000068.7 |
2 |
14725247 |
+ |
Cacnb2 |
NNNCAAGCATGATCTTTCTA |
NGT |
2 |
0.0108 |
Tier II |
Other clones with same target sequence:
(none)
