Construct: sgRNA BRDN0001147191
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTATACGAACATCCAGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CROT (54677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77706
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
87361421 |
- |
CROT |
NNNTATACGAACATCCAGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
79094511 |
+ |
SYT1 |
NNNTATACAAACATACAGAT |
NGG |
2 |
0.1429 |
Tier II |
| 3 |
NC_000004.12 |
4 |
16720702 |
- |
LDB2 |
NNNTATACGAACATCCATAT |
NGC |
2 |
0.0148 |
Tier II |
| 4 |
NC_000009.12 |
9 |
69831975 |
+ |
CFAP95 |
NNNTATAAGAACAGCCAGAT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000012.12 |
12 |
79094511 |
+ |
LOC105369863 |
NNNTATACAAACATACAGAT |
NGG |
2 |
0.1429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
57388366 |
- |
Slc22a22 |
NNNTATATGAACATCCTGAT |
NGG |
2 |
0.1167 |
Tier II |
| 2 |
NC_000074.6 |
8 |
25074148 |
+ |
Plekha2 |
NNNTATACGAGCATGCAGAT |
NGG |
2 |
0.0325 |
Tier II |
| 3 |
NC_000067.6 |
1 |
183800907 |
+ |
Gm34068 |
NNNTATATGAACATCCAGAA |
NGG |
2 |
0.4922 |
Tier III |
Other clones with same target sequence:
(none)
