Construct: sgRNA BRDN0001147193

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATCTTCAACAAGAGGACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK2 (9261)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77206

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 206685486 + MAPKAPK2 NNNCTTCAACAAGAGGACCC NGG 0 1.0 Tier I
2 NC_000006.12 6 142771338 - HIVEP2 NNNCTTCAGCAAGAGGACCC NGG 1 0.6667 Tier I
3 NC_000006.12 6 138692746 - NHSL1 NNNCTTCCACCAGAGGACCC NGG 2 0.1714 Tier I
4 NC_000008.11 8 138132762 + FAM135B NNNCTTCAACCAGAGGGCCC NGG 2 0.0706 Tier I
5 NC_000001.11 1 12468423 + VPS13D NNNCTTTAACAAGAGGACCC NGG 1 0.8125 Tier II
6 NC_000012.12 12 111250424 + CUX2 NNNCTAAAACAAGAGGACCC NGG 2 0.65 Tier II
7 NC_000009.12 9 131583389 + RAPGEF1 NNNCTTGAACAAGAAGACCC NGG 2 0.4429 Tier II
8 NC_000017.11 17 49711110 + FAM117A NNNCATCAAAAAGAGGACCC NGG 2 0.4333 Tier II
9 NC_000010.11 10 119845315 + MCMBP NNNATTCAACAAGAGGACTC NGG 2 0.3887 Tier II
10 NC_000016.10 16 81879601 + PLCG2 NNNGTTCAGCAAGAGGACCC NGG 2 0.3333 Tier II
11 NC_000022.11 22 38819385 + NPTXR NNNCTTCACCAAGGGGACCC NGG 2 0.2667 Tier II
12 NC_000006.12 6 138692746 - LOC124900217 NNNCTTCCACCAGAGGACCC NGG 2 0.1714 Tier II
13 NC_000008.11 8 11425601 + FAM167A NNNCTTCATCAAGAGGACCC NAG 2 0.1556 Tier II
14 NC_000001.11 1 6252845 + GPR153 NNNCTTCACCAAGAGGACCC NAG 2 0.1481 Tier II
15 NC_000017.11 17 42413269 + CAVIN1 NNNCTTCATCAAGAGGGCCC NGG 2 0.1059 Tier II
16 NC_000004.12 4 173511520 + SCRG1 NNNCTTAAACAAGAGGTCCC NGG 2 0.1 Tier II
17 NC_000018.10 18 52719965 + DCC NNNCTTCAACAAGAAGACCC NGA 2 0.0654 Tier II
18 NC_000011.10 11 108179105 + NPAT NNNCTTCAACAAGAGGACCA NGA 2 0.0347 Tier II
19 NC_000018.10 18 13303803 - LDLRAD4 NNNCTTCAACAAGAGGTCCC NAG 2 0.0346 Tier II
20 NC_000003.12 3 156372711 + KCNAB1 NNNCTTCAACAAGAGGACAC NGT 2 0.0069 Tier II
21 NC_000012.12 12 46775480 - SLC38A4 NNNCTTCAACAAGAGCACCT NGG 2 0.0 Tier II
22 NC_000005.10 5 4862539 + LOC107986400 NNNTTTCAAAAAGAGGACCC NGG 2 0.6933 Tier III
23 NC_000006.12 6 138692746 - NHSL1-AS1 NNNCTTCCACCAGAGGACCC NGG 2 0.1714 Tier III
24 NC_000008.11 8 11425601 + FAM167A-AS1 NNNCTTCATCAAGAGGACCC NAG 2 0.1556 Tier III
25 NC_000004.12 4 173511520 + LOC101928409 NNNCTTAAACAAGAGGTCCC NGG 2 0.1 Tier III
26 NC_000020.11 20 24820036 + LOC107985402 NNNCTTCAACGAGAGGACCC NTG 2 0.0253 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 77698130 + Tspear NNNCTACAGCAAGAGGACCC NGG 2 0.5778 Tier II
2 NC_000068.7 2 53695009 + Gm38481 NNNCTACAACAAGAGGATCC NGG 2 0.5571 Tier II
3 NC_000079.6 13 8891732 - Idi1 NNNCTACAACAAGAGGATCC NGG 2 0.5571 Tier II
4 NC_000084.6 18 14740249 + Psma8 NNNCTTCAACAAGAGGACCA NGG 1 0.5 Tier II
5 NC_000081.6 15 83779482 + Mpped1 NNNGCTCAACAAGAGGACCC NGG 2 0.5 Tier II
6 NC_000076.6 10 31828547 + Nkain2 NNNCTTCAGCAGGAGGACCC NGG 2 0.4815 Tier II
7 NC_000070.6 4 82303204 + Nfib NNNCTTCAACAAGGGAACCC NGG 2 0.4667 Tier II
8 NC_000073.6 7 116083983 + 1110004F10Rik NNNCTCCAACAAGAGGACTC NGG 2 0.4196 Tier II
9 NC_000074.6 8 84234563 - Zswim4 NNNCATCAGCAAGAGGACCC NGG 2 0.3333 Tier II
10 NC_000076.6 10 21892246 + Sgk1 NNNCTGCAACAAGTGGACCC NGG 2 0.3048 Tier II
11 NC_000075.6 9 76108617 + Hmgcll1 NNNCTTCAACAAGTGGAACC NGG 2 0.2872 Tier II
12 NC_000078.6 12 111855814 + Ppp1r13b NNNCAGCAACAAGAGGACCC NGG 2 0.2857 Tier II
13 NC_000070.6 4 35020369 + Mob3b NNNCTTCTACAAGAGGACTC NGG 2 0.1978 Tier II
14 NC_000069.6 3 96855520 - Pdzk1 NNNCTTCAACAAGAGAGCCC NGG 2 0.1765 Tier II
15 NC_000072.6 6 56884023 + Nt5c3 NNNCTTCCACCAGAGGACCC NGG 2 0.1714 Tier II
16 NC_000077.6 11 103109413 + Acbd4 NNNCTTCATCAAGAGGCCCC NGG 2 0.1059 Tier II
17 NC_000071.6 5 108426012 - Pde6b NNNCTTCAACAAGAGGATGC NGG 2 0.0804 Tier II
18 NC_000074.6 8 32513968 + Nrg1 NNNCTTCAACAAGAGGAGCC NAG 2 0.0346 Tier II
19 NC_000073.6 7 16117857 + Napa NNNCTTCAACAAAAGGACCC NGT 2 0.0149 Tier II
20 NC_000068.7 2 121805838 - Frmd5 NNNCTTCAACCAGAGGACCC NGC 2 0.0089 Tier II
21 NC_000082.6 16 43892518 + Ccdc191 NNNCTTCAACAAGAGGACCC NTT 2 0.0 Tier II
22 NC_000071.6 5 91724303 + Gm36222 NNNATTCAACAAGAGAACCC NGG 2 0.8421 Tier III
23 NC_000072.6 6 7927707 - Gm35246 NNNCTGCAACAAGAGAACCC NGG 2 0.5714 Tier III
24 NC_000073.6 7 116083983 + 1700003G18Rik NNNCTCCAACAAGAGGACTC NGG 2 0.4196 Tier III
25 NC_000073.6 7 116083983 + Gm4353 NNNCTCCAACAAGAGGACTC NGG 2 0.4196 Tier III
26 NC_000071.6 5 108426012 - Gm34204 NNNCTTCAACAAGAGGATGC NGG 2 0.0804 Tier III
27 NC_000080.6 14 104814527 + D130009I18Rik NNNCTTCAACAAGAGGAGTC NGG 2 0.0615 Tier III
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Other clones with same target sequence:

(none)