Construct: sgRNA BRDN0001147194
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCTCTATGACTTCACGGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRMS (6725)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76647
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63547275 |
- |
SRMS |
NNNTCTATGACTTCACGGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
17603699 |
+ |
NHS |
NNNTCTATGACTTCACACCG |
NGG |
2 |
0.4444 |
Tier II |
| 3 |
NC_000015.10 |
15 |
43928170 |
- |
FRMD5 |
NNNACTATGTCTTCACGGCG |
NGG |
2 |
0.4202 |
Tier II |
| 4 |
NC_000006.12 |
6 |
95929209 |
- |
LOC107986626 |
NNNTCTATGACTTCAAGGCA |
NGG |
2 |
0.9375 |
Tier III |
| 5 |
NC_000001.11 |
1 |
15409526 |
+ |
EFHD2-AS1 |
NNNATTATGACTTCACGGCG |
NGG |
2 |
0.303 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
55762797 |
+ |
Ltb4r2 |
NNNTCTATGTCTTCACTGCG |
NGG |
2 |
0.2206 |
Tier I |
| 2 |
NC_000075.6 |
9 |
99637509 |
- |
Dzip1l |
NNNTCTATGCTTTCACGGCG |
NGG |
2 |
0.1026 |
Tier I |
| 3 |
NC_000070.6 |
4 |
10997900 |
+ |
Plekhf2 |
NNNTCTATGACTTCACAGCT |
NGG |
2 |
0.6533 |
Tier II |
| 4 |
NC_000069.6 |
3 |
26190644 |
- |
Nlgn1 |
NNNTCTATGACTTCACAGTG |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000072.6 |
6 |
97493407 |
- |
Frmd4b |
NNNTCTATGACCTCATGGCG |
NGG |
2 |
0.2915 |
Tier II |
| 6 |
NC_000079.6 |
13 |
112769834 |
- |
Gm41067 |
NNNTCTATGACATCACGGTG |
NGG |
2 |
0.3692 |
Tier III |
Other clones with same target sequence:
(none)
